This assay is used to disrupt gene function by creating an intragenic gene fusion.

The Ames test is a biological assay to assess the mutagenic potential of chemical compounds.

Another setup commonly used for measuring chemotaxis and chemokinesis utilizes the under-agarose cell migration assay, whereby a layer of agarose gel is placed between a cell population and a chemoattractant.

A novel assay that mimics dry bacterial exposure to touch surfaces was developed because this test method is thought to more closely replicate real world touch surface exposure conditions.

In fact, the enzyme-linked immunosorbent assay ( ELISA ), which uses antibodies, is currently one of the most sensitive tests modern medicine uses to detect various biomolecules.

A subsequent review of these tests by the Federal Insecticide, Fungicide, and Rodenticide Act Scientific Advisory Panel points out that while " the negative results decrease the probability that the Cry9C protein is the cause of allergic symptoms in the individuals examined ... in the absence of a positive control and questions regarding the sensitivity and specificity of the assay, it is not possible to assign a negative predictive value to this "

Culture remains useful in selected circumstances and is currently the only assay approved for testing non-genital specimens.

Genotyping is the process of elucidating the genotype of an individual with a biological assay.

The basic idea of microfluidic biochips is to integrate assay operations such as detection,

Screening for PKU is done with bacterial inhibition assay ( Guthrie test ), immunoassays using fluorometric or photometric detection, or amino acid measurement using tandem mass spectrometry ( MS / MS ).

#" Elevated serum prolactin assay, when measured in the appropriate clinical setting at 10 to 20 minutes after a suspected event, is a useful adjunct for the differentiation of generalized tonic-clonic or complex partial seizure from psychogenic nonepileptic seizure among adults and older children ( Level B ).

The effort expended in separating a mass F of feed of assay xf into a mass P of product assay xp and waste of mass W and assay xw is expressed in terms of the number of separative work units needed, given by the expression SWU

This easy assay, which can be seen with the naked eye, without the aid of instruments, is possible because catalase has a very high specific activity, which produces a detectable response.

Bioassay ( commonly used shorthand for biological assay ), or biological standardization is a type of scientific experiment.

This is also known as the " all or none " response assay.

This assay is applied when the sample size is too small.

Since the assay does not involve the recording of concentration response curve, the sensitivity of the preparation is not taken into consideration.

A fasting serum insulin level of greater than the upper limit of normal for the assay used ( approximately 60 pmol / L ) is considered evidence of insulin resistance.

An example that is often used in teaching laboratories is a controlled protein assay.

The assay is a colorimetric assay in which a spectrophotometer can measure the amount of protein in samples by detecting a colored complex formed by the interaction of protein molecules and molecules of an added dye.

The Poly-A tail of the post transcription mRNA can be taken advantage of with oligo ( dT ) beads in an affinity chromatography assay.

Serologically an enzyme linked immunosorbent assay during the acute phase of the disease using specific IgM against yellow fever or an increase in specific IgG-titer ( compared to an earlier sample ) can confirm yellow fever.

Unlike other spectrophotometric wet lab assay formats where the same reaction well ( e. g. a cuvette ) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, so are not easily reusable.

As a " wet lab " analytic biochemistry assay, ELISA involves detection of an " analyte " ( i. e. the specific substance whose presence is being quantitatively or qualitatively analyzed ) in a liquid sample by a method that continues to use liquid reagents during the " analysis " ( i. e. controlled sequence of biochemical reactions that will generate a signal which can be easily quantified and interpreted as a measure of the amount of analyte in the sample ) that stays liquid and remains inside a reaction chamber or well needed to keep the reactants contained ; It is opposed to " dry lab " that can use dry strips-and even if the sample is liquid ( e. g. a measured small drop ), the final detection step in " dry " analysis involves reading of a dried strip by methods such as reflectometry and does not need a reaction containment chamber to prevent spillover or mixing between samples.

SEC can also assay protein tertiary structure, as it measures the hydrodynamic volume ( not molecular weight ), allowing folded and unfolded versions of the same protein to be distinguished.

BERA is used primarily in biosensor applications in order to assay analytes that can interact with the immobilized cells by changing the cell membrane potential.

The authors of the study themselves, as well as several others, pointed out the low assay sensitivity of this study, and how only limited conclusions can be drawn from its results.

These can be detected using an enzyme-linked immunosorbent assay ( ELISA ) immunological test, which screens for the presence of β < sub > 2 </ sub > glycoprotein 1 dependent anticardiolipin antibodies ( ACA ).

Testing of overall fibrinolysis can be measured by a euglobulin lysis time ( ELT ) assay.

Proteins can be detected down to femtomole quantities, well below the detection limit for most assay systems.

In order to study the function of a lymphocyte by virtue of the proteins it generates, other scientific techniques like the ELISPOT or secretion assay techniques can be used.

The test for pregnancy which can give the quickest result after fertilisation is a rosette inhibition assay for early pregnancy factor ( EPF ).

Type 2N can only be diagnosed by performing a " factor VIII binding " assay.

Cytotoxicity can also be monitored using the 3 -( 4, 5-Dimethyl-2-thiazolyl )- 2, 5-diphenyl-2H-tetrazolium bromide ( MTT ) or MTS assay.

Cytotoxicity can also be measured by the sulforhodamine B ( SRB ) assay, WST assay and clonogenic assay.

It has been shown that a microsystem-based assay can mimics the in vivo micro-environment of ovule fertilization by pollen tubes in Arabidopsis thaliana.

The HIV proteins used in western blotting can be produced by recombinant DNA in a technique called recombinant immunoblot assay ( RIBA ).

Reporter genes can also be used to assay for the expression of the gene of interest, which may produce a protein that has little obvious or immediate effect on the cell culture or organism.

Reporter genes can be used to assay for the activity of a particular promoter in a cell or organism.

Enzyme-linked immunosorbent assay ( ELISA ), is a popular format of a " wet-lab " type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay ( EIA ) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.

DHFR has been used as a tool to detect protein-protein interactions in a protein-fragment complementation assay ( PCA ).

Other related techniques include using antibodies to detect proteins in tissues and cells by immunostaining and enzyme-linked immunosorbent assay ( ELISA ).

K562 is highly sensitive to lysis by human NK cells and, over the decades, the K562 < sup > 51 </ sup > Chromium-release assay has become the most commonly used assay to detect human NK functional activity.

The Guthrie test, also known as the Guthrie bacterial inhibition assay, is a medical test performed on newborn infants to detect phenylketonuria, a disorder error of amino acid metabolism.

The Guthrie test is a semiquantitative assay designed to detect elevated blood levels of the amino acid phenylalanine, using the ability of phenylalanine to facilitate bacterial growth in a culture medium with an inhibitor.

The Guthrie assay is sensitive enough to detect serum phenylalanine levels of 180-240 μmol / L ( 3 – 4 mg / dL ).

Polyhistidine-tagging can be used to detect protein-protein interactions in the same way as a pull-down assay.

* Cell viability assays: resazurin method, ATP test, Ethidium homodimer assay ( detect dead or dying cells ), Bacteriological water analysis, Clonogenic assays, ...

* Tetramer assay detect the presence of antigen specific T-cells

Immunohistochemical staining for antibodies against T. whipplei has been used to detect the organism in a variety of tissues, and a PCR-based assay is also available.

Robert Guthrie developed a simple method using a bacterial inhibition assay that could detect high levels of phenylalanine in blood shortly after a baby was born.

The simplifying assumption made in this approach is that any N compounds in the same well will not typically interact with each other, or the assay target, in a manner that fundamentally changes the ability of the assay to detect true hits.

Three monoclonal antibodies ( T16, C26, and AE-1 ), capable of recognizing membrane and cytoskeletal antigens expressed by epithelial cells to detect tumour cells, were used in the assay.

Enzyme-linked immunosorbent assay ( EIA ) is used in diagnostic laboratories to detect ANCAs.

Samples of rocks or soil may be collected for geochemical laboratory assay, to determine metal content or detect geochemical anomalies.

These are constructed by immobilising large numbers of purified proteins and are used to identify protein-protein, protein-DNA, protein-RNA, protein-phospholipid, and protein-small molecule interactions, to assay enzymatic activity and to detect antibodies and demonstrate their specificity.

human IgG onto a 600-angstrom silver film, and used the assay to detect anti-human IgG in water solution.