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# Direct Mutation Targeting: The siRNAs are designed to perfectly match mutant alleles but contain one or more mismatches with wild-type alleles, leading to specific degradation of the matching, mutant transcripts.
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# Indirect Mutation Targeting: The siRNA approach will not work if the mutant alleles are too similar to wild type.
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# Targeting exon skipped transcripts: If the problem in the gene lies in aberrant splicing post-transcription, siRNA can be designed to target the unnatural exon-exon interface arising as a result of such alternative splicing.
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