For instance, laser microscopy focused on biological applications uses ultrashort pulse lasers, or femtosecond lasers, in a number of techniques labeled as nonlinear microscopy, saturation microscopy, and multiphoton fluorescence microscopy.

For example, indirect immunofluorescence will allow for fluorescence colocalization and demonstration of location.

For example, visible light is emitted by the coupling of electronic states in atoms and molecules ( then the phenomenon is called fluorescence or phosphorescence ).

For example, from a data set comprising fluorescence spectra from a series of samples each containing multiple fluorophores, multivariate curve resolution methods can be used to extract the fluorescence spectra of the individual fluorophores, along with their relative concentrations in each of the samples, essentially unmixing the total fluorescence spectrum into the contributions from the individual components.

For a circular bleach spot of radius w and diffusion-dominated recovery, the fluorescence is described by an equation derived by Soumpasis ( which involves modified Bessel functions and )

For the WAGR syndrome, high-resolution cytogenetic analysis and fluorescence in situ hybridization ( FISH ) can be utilized to identify deletions within chromosome band 11p13, where both the PAX6 and WT1 genes are located.

For example, a technique known as calcium imaging measures fluorescence intensity of calcium-sensitive dyes to asses intracellular calcium levels.

For example, by creating a fusion gene of a protein of interest and green fluorescent protein, the protein of interest may be observed in cells or tissue using fluorescence microscopy.

For example, FA may reveal areas of hyperfluorescence, indicating that the RPE has lost some of its integrity, allowing the underlying fluorescence from the choroid to be more visible.

For instance, there is a phase difference between a sinusoidally varying light emitting diode ( LED ) signal of frequency f and the fluorescence that results ( see figure ).

For field ion microscopy a gas is introduced, known as the imaging gas, into the chamber at very low pressures.

For identification by light microscopy, a scraping or swab of the affected area is placed on a microscope slide.

For electron microscopy, the most commonly used fixative is glutaraldehyde, usually as a 2. 5 % solution in phosphate buffered saline.

For light microscopy, paraffin wax is most frequently used.

For light microscopy, a steel knife mounted in a microtome is used to cut 10-micrometer-thick tissue sections which are mounted on a glass microscope slide.

For transmission electron microscopy, a diamond knife mounted in an ultramicrotome is used to cut 50-nanometer-thick tissue sections which are mounted on a 3-millimeter-diameter copper grid.

For interior imaging, the quality of the image is greatly enhanced over simple microscopy because image information from multiple depths in the specimen is not superimposed.

For illumination techniques like dark field, phase contrast and differential interference contrast microscopy additional optical components must be precisely aligned in the light path.

For specialist purposes glass knives are still made and used today, particularly for cutting thin sections for electron microscopy in a technique known as microtomy.

For a different type of microscopy that also leverages light scattering against a dark background see dark field microscopy.

For example, the first application of serial block-face scanning electron microscopy was on rodent cortical tissue.

For a given numerical aperture ( NA ), the resolution of microscopy for flat objects under coherent illumination can be improved using interferometric microscopy.

For a while microscopy suffered in its reputation, but by 1840 histology was a recognised discipline, and in time the view of Hodgkin and Lister that " globules " were optical artefacts became accepted.

For more than thirty years he edited and published The Microscope, an international quarterly journal of microscopy.

For example, rhombomeres are detectable by light microscopy in Swiss / Webster strain of mice for up to embryonic day ( E ) 10. 5, but they disappear at E11. 5.

For example observing a tissue sample prepared with a fluorescent DNA stain by fluorescent microscopy only reveals the organisation of the DNA within the cells and reveals nothing else about the cell morphologies.

For more detailed analysis of much smaller areas in a thin section, FTIR microscopy can be used for sample inspection.

For multilayer boards, those with 3 layers or more, drilling typically produces a smear of the high temperature decomposition products of bonding agent in the laminate system.

For this reason, there are often unproven rumors, that are termed, smear campaign, about many politicians.

For example, the NSW Department of Land and Water Conservation, ( 1991 ) advocates: increasing organic content by incorporating pasture phases into cropping rotations ; reducing or eliminating tillage and cultivation in cropping and pasture activities ; avoiding soil disturbance during periods of excessive dry or wet when soils may accordingly tend to shatter or smear, and ; ensuring sufficient ground cover to protect the soil from raindrop impact.

For example, a medical procedure called a buccal smear uses a small brush or cotton swab to collect a sample of cells from the inside surface of the cheek.

For example, Ralph Nader was the victim of a smear campaign during the 1960s, when he was campaigning for car safety.

For samples of loose cells ( as with a blood smear or a pap smear ) the sample can be directly applied to a slide.

: Cyrano: "... For there is one thing I have left, void of smear or stain, and I take it with me despite you.

For instance, in malaria, the number of plasmodia can be counted using an optical microscope, on a special thick film ( for low parasitemias ) or thin film blood smear ( for high parasitemias ).

For proper bonding of resin composite restorations dentin should be etched with acids to remove a smear layer, created during mechanical treatment with dental bore, and expose some of the collagen network or organic matrix of dentin.

For example Test 3, E 1 refers to a specimen which has been washed by procedure `` 3 ( '' ( at 160-degrees-F ) for a total of 60 minutes in the machine, has been dried in a tumble dryer by procedure `` E '' and has been subjected to restorative forces on the Tension Presser by procedure `` 1 ''.

For weeks after the battle, bodies washed up along the Egyptian coast, decaying slowly in the intense dry heat.

For instance, on November 30, 2006, a container washed ashore on the Outer Banks of North Carolina USA, along with thousands of bags of its cargo of Doritos Chips.

For years afterwards the city's residents remained so proud of the street that they washed it every week with a fire hose.

For Paris, the only hard figure is a payment by the city to workmen for collecting and burying 1, 100 bodies washed up on the banks of the Seine downstream from the city in one week.

For this crime they were set adrift at sea ; they washed ashore on a windswept island, which they named " Albion "-after Alba.

For assessment with flow cytometry the washed cells are incubated with the chosen probe, ( possibly washed again ) and then sampled in a flow cytometer.

For the same reason the coat should not be washed too often, as detergents take away the natural skin oils.

For " washed the spider out " sweep the hands to the side.

For a time, alewives, which often exhibit seasonal die-offs, washed up in windrows on the shorelines of the Great Lakes.

For example, some investigators state that where sand had washed away between the seams, another course of blocks can be seen along with small blocks underlying these blocks.

For example, suppose a match is scheduled for five days, but the first day is washed out because of rain.

For example, tree rings, ice cores, and corals generally show variation on an annual time scale, but borehole reconstructions rely on rates of thermal diffusion, and small scale fluctuations are washed out.

For example, he washed cars, picked lettuce in the Imperial Valley, and served as a bouncer at a Santa Monica pier.

For these reasons, yixing teawares should never be washed using detergents, but rather with water only, and connoisseurs recommend using each tea vessel for one kind of tea ( white, green, oolong, or black ) or sometimes even one variety of tea only.

For ten years, with no money from the University for Yeagley's salary, team travel expenses, recruiting or uniforms, Yeagley, his wife Marilyn and the players lined the field, hung signs on campus and washed the players ' uniforms.