* In situ hybridization shows which cells are expressing a particular RNA transcript.

More commonly, autoradiography is used to visualize the locations to which a radioactive substance has been transported within the body, such as cells in S phase ( undergoing DNA replication ) which incorporate tritiated thymidine, or sites to which radiolabeled nucleic acid probes bind in in situ hybridization.

Other advanced techniques, such as nonradioactive in situ hybridization, can be combined with immunochemistry to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification ( especially alkaline phosphatase and tyramide signal amplification ).

Epigenetic research uses a wide range of molecular biologic techniques to further our understanding of epigenetic phenomena, including chromatin immunoprecipitation ( together with its large-scale variants ChIP-on-chip and ChIP-Seq ), fluorescent in situ hybridization, methylation-sensitive restriction enzymes, DNA adenine methyltransferase identification ( DamID ) and bisulfite sequencing.

Top: Both X-chromosome territories are detected by Fluorescence in situ hybridization | FISH.

# Tissue-sequence correlates should be sought at the cellular level: efforts should be made to demonstrate specific in situ hybridization of microbial sequence to areas of tissue pathology and to visible microorganisms or to areas where microorganisms are presumed to be located.

Karyogram from a human female lymphocyte probed for the Alu sequence using Fluorescent in situ hybridization | FISH.

Still, the determination of the primary tumor can often be very difficult, and the pathologist may have to use several adjuvant techniques, such as immunohistochemistry, FISH ( fluorescent in situ hybridization ), and others.

* In situ hybridization: detects the presence of a transcript

Transcription factories can also be localized using fluorescence in situ hybridization or marked by antibodies directed against polymerases.

Examples of procedures that use oligonucleotides include DNA microarrays, Southern blots, ASO analysis, fluorescent in situ hybridization ( FISH ), and the synthesis of artificial genes.

Top: Both X-chromosome territories are detected by Fluorescence in situ hybridization | FISH.

If a physician suspects a case of Williams syndrome, the diagnosis is confirmed using one of two possible genetic tests: micro-array analysis or the fluorescent in situ hybridization ( FISH ) test.

mRNA expression of the reporter can be visualized by in situ hybridization, which provides a more direct measure of enhancer activity, since it is not subjected to the complexities of translation and protein folding.

The technique uses colorimetric in situ hybridization to map gene expression.

Another technique, called in situ hybridization, or ISH, is used to view gene expression patterns as in situ hybridization images.

In situ hybridization uses synthetic RNA probes that attach ( hybridize ) selectively to complementary mRNA transcripts of DNA exons in the cytoplasm, to visualize genomic readout, that is, distinguish active gene expression, in terms of mRNA rather than protein.

It includes routine analysis of G-banded chromosomes, other cytogenetic banding techniques, as well as molecular cytogenetics such as fluorescent in situ hybridization ( FISH ) and comparative genomic hybridization ( CGH ).

Hybridizing them to chromosomal preparations using existing techniques came to be known as fluorescence in situ hybridization ( FISH ).

Fluorescent in situ hybridization refers to using fluorescently labeled probe to hybridize to cytogenetic cell preparations.

* Molecular biology techniques such as DNA microarrays, in situ hybridization, combined to PCR, Computational genomics, and Transfection ; Cell fractionation or Immunoprecipitation

These foci are negative for TSV by in situ hybridization ( ISH ) using a TSV-specific cDNA probe.

RNA ISH ( RNA in situ hybridization ) is used to measure and localize RNAs ( mRNAs, lncRNAs and miRNAs ) within tissue sections, cells and circulating tumor cells ( CTCs ).

It is prepared in situ by dissolving potassium dichromate in concentrated sulfuric acid, which is then used to wash the apparatus.

The four pillars set up by Cão on his two voyages have all been discovered in situ, and the inscriptions on two of them from Cape Santa Maria and Cape Cross, dated 1482 and 1485 respectively, are still to be read and have been printed ; the Cape Cross padrão is now at Kiel ( replaced on the spot by a granite facsimile ); those from the Congo estuary and the more southerly Monte Negro are in the Museum of the Lisbon Geographical Society.

The carboxylate salt is often generated in situ.

* Hügelkultur is concerned with growing plants on piles of rotting wood, as a form of raised bed gardening and composting in situ.

This is seen in cases such as the genus Haloarcula, which is estimated to make up less than 0. 1 % of the in situ community but commonly appears in isolation studies.

While preservation in situ is not assured, material that has survived underwater and is then recovered to land is typically in an unstable state and can only be preserved using highly specialised conservation processes.

Equipment is available to extract in situ resources ( e. g., water, air ) from the Martian ground and atmosphere.

Although the young woman's coffin was robbed in antiquity, the other remained in situ and undisturbed, and is now on display at the site.

Sometimes the biomass is regarded as the natural mass of organisms in situ, just as they are.

William S. Burroughs wrote about the candiru in his 1959 novel Naked Lunch, describing it as " a small eel-like fish or worm about one-quarter inch through and two inches long patronizing certain rivers of ill repute in the Greater Amazon Basin, will dart up your prick or your asshole or a woman's cunt faute de mieux, and hold himself there by sharp spines with precisely what motives is not known since no one has stepped forward to observe the candiru's life-cycle in situ.

There are a few remains of the Roman city visible, such as parts of the city walls, a hypocaust still in situ under a mosaic floor and a theatre, which is on land belonging to the Earl of Verulam-as well as items in the Museum ( below ).

Under cutting occurs where contexts are not excavated fully and some remainder of the context is left in situ masking the nature of the underlying contexts.

Another jar from the same tomb — which was discovered in situ by a 1935 – 1936 Metropolitan Museum of Art expedition on a hillside near Thebes — was stamped with the seal of the ' God's Wife Hatshepsut ' while two jars bore the seal of ‘ The Good Goddess Maatkare ’</ ref > The dating of the amphorae, " sealed into the burial chamber by the debris from Senenmut's own tomb ," is undisputed which means that Hatshepsut was acknowledged as the king of Egypt by Year 7 of her reign.

It is better to sow them as soon as ripe, either in cold frames or seedbeds outdoors, where they can be left in situ for 1 to 2 years before being planted in their permanent positions, or in pots, where the plants can be put out into their permanent positions in summer or autumn.

A catalytic amount of may be used for the transformation using phosphorus and bromine ; is formed in situ.

In general, acetoacetic acid is generated at 0 ° C and used in situ immediately.

Along this route there is an accumulation of vintage, in situ roadside Americana advertising signage that is unsurpassed in the area, including " Venture ", " Veteran's Carpet " and " The Night Spot ".

It is possible that one purpose of the Villa was as an art gallery, as inventories show more than 167 paintings hanging in situ at Chiswick House in Lord Burlington's lifetime, many purchased on his two Grand Tours of Europe.

His conclusion is that dialect differences formed in situ, and not as a result of different waves of migration.