A sample of Fraction Af from group O plasma was dissolved in starting buffer, dialyzed against this buffer and subjected to chromatography using the gradient shown in Fig. 2.

The precipitate was washed twice with an 80% saturated solution of Af, dissolved in a small quantity of 0.1 M neutral phosphate buffer, dialyzed against cold distilled water till free from ammonium ions, and lyophilized using liquid nitrogen.

The greatest source of trouble was rain which had repeatedly flowed from openings above, soaking the surface and leaving streaks of dissolved lime, very conspicuous even after cleaning, particularly in the `` Landing of Columbus '', `` Oglethorpe and the Indians '', and `` Yorktown ''.

The dissolved oxygen in the aeration unit was consistently high until January 29, 1961.

It was approximately one month before the belt problem was noticed and corrected, but at no time was there a deficiency of dissolved oxygen.

The maximum rate of oxygen transfer at 1.0 mg/l dissolved oxygen was calculated as 220 lb/day at a maximum rate of 9.3 Aj.

Even before it was formally dissolved in 1912, the A.L.A.M. was succeeded by the Automobile Board of Trade, the direct lineal ancestor of the present-day Automobile Manufacturers Association.

Mary was free to marry again, not knowing that the Sturch had secretly given her a divorce, thinking that death had dissolved her marriage.

After the end of the Greek-Persian wars the cities on the coasts became part of the Delian League, which was, however, later dissolved.

After cyclotron irradiation, the coating was dissolved with nitric acid, and then precipitated as the hydroxide using concentrated aqueous ammonia solution.

The residue was dissolved in perchloric acid.

Alumni played its last game in 1911 and was definitely dissolved on April 24, 1913.

However, this situation changed drastically when Alexios ' first son John II Komnenos was born in 1087: Anna's engagement to Constantine was dissolved, and she was moved to the main Palace to live with her mother and grandmother.

Although various Ambrosians were given the title of Blessed in recognition of their holiness: Antonio Gonzaga of Mantua, Filippo of Fermo, and Gerardo of Monza, the order was eventually dissolved by Pope Innocent X in 1650.

By 431 BC Athens ' heavy-handed control of the Delian League would prompt the outbreak of the Peloponnesian War ; the League was dissolved upon the war's conclusion in 404 BC.

The marriage was dissolved in 1929 and Shapiro / Tanaroff acknowledged his paternity, but never married Hanka Grothendieck.

After the Arab Islamic conquest in the 7th century AD Assyria was dissolved as an entity.

* 1999 – The German Bundestag returns to Berlin, the first German parliamentary body to meet there since the Reichstag was dissolved in 1945.

With the Act of Mediation in 1803, the Canton of Baden was dissolved.

A new constitution was adopted in 2007 ( the Virgin Islands Constitution Order, 2007 ) and came into force when the Legislative Council was dissolved for the 2007 general election.

Born in Boston, Colony of Massachusetts, to David Yale ( 1613 – 1690 ) and Ursula Knight ( 1624 – 1698 ), Yale was the grandson of Ann Lloyd ( 1591 – 1659 ), who after the death of her first husband, Thomas Yale ( 1590 – 1619 ) in Chester, Cheshire, England, married Governor Theophilus Eaton ( 1590 – 1658 ) of New Haven Colony.

The Commonwealth ( 1649 – 53 ) was founded on the execution of Charles I in 1649, and was followed by the two Protectorates of Oliver Cromwell ( 1653 – 58 ), and his son Richard Cromwell the first ( 1658 – 59 ).

He graduated M. A., and was chosen professor at King's College, Aberdeen, in 1658.

Their last meeting was in 1658 at Hampton Court, though they could not speak for long or meet again because of the Protector's worsening illness — Fox even wrote that " he looked like a dead man ".

She was ten years his senior and had eight children ( all but one of them Quakers ) by her first husband, Thomas Fell, who had died in 1658.

After he died in 1658, his son Richard Cromwell succeeded him in the office but he was forced to abdicate within a year.

It was also used on the Commonwealth Jack of Oliver Cromwell, issued in 1649 and on the Protectorate Jack issued in 1658 as well as on the Lord Protector's Standard issued on the succession of Richard Cromwell in 1658.

Jacques Abbadie was born at Nay, Béarn, probably in 1654, although 1657 and 1658 have been given ; he is " most probably the Jacques Abbadie who was the third child of Violente de Fortaner and Pierre Abbadie, baptized on 27 April 1654.

Locke was awarded a bachelor's degree in 1656 and a master's degree in 1658.

The first known appearance of the spelling Krakatau was by Wouter Schouten, who passed by " the high tree-covered island of Krakatau " in October 1658.

The current university was however not founded until 1666 after Sweden had won Scania in the 1658 peace agreement with Denmark.

After Richard Cromwell, who had succeeded his father Oliver as Lord Protector in 1658, was effectively deposed by an officers ' coup in April, 1659, the officers re-summoned the Rump Parliament to sit.

The region was subject to frequent raids by Dutch, French and British pirates, with the city of Granada being invaded twice, in 1658 and 1660.

This success was followed by The Cruelty of the Spaniards in Peru ( 1658 ) and The History of Sir Francis Drake ( 1659 ).

In his Memoires ( 1658 ), Francis Osborne writes of " the last great Earle of Oxford, whose Lady was brought to his bed under the notion of his Mistris, and from such a virtuous deceit she ( Oxford's youngest daughter ) is said to proceed " ( p. 79 ).

Joseph Clement was not only the candidate of Emperor Leopold I ( 1658 – 1705 ) but of all European rulers, with the exception of the King of France and his supporter, King James II of England ( 1685 – 88 ).

In late 1662 the first part of Hudibras, which he began writing when lodging at Holborn, London in 1658 and continued to work on while in Ludlow, was published, and the other two in 1664 and 1678 respectively.

Shah Jahan ( also spelled Shah Jehan, Shahjehan ,(, Persian: شاه جهان ) ( January 5, 1592 – January 22, 1666 ) was emperor of the Mughal Empire in South Asia from 1628 until 1658.

In 1658, he fell ill and was confined by his son Emperor Aurangzeb in Agra Fort until his death in 1666.

After the Treaty of Roskilde on 26 February 1658, Trondheim and the rest of Trøndelag, became Swedish territory for a brief period, but the area was reconquered after 10 months.

After the pro-Polish hetman Ivan Vyhovsky came to power and a civil war broke out, In 1658 Poltava, under polkovnyk Martyn Pushkar, was the leading town of the rebels.