Once the genetic representation and the fitness function are defined, a GA proceeds to initialize a population of solutions ( usually randomly ) and then ( usually ) to improve it through repetitive application of the mutation, crossover, inversion and selection operators.

Once a mutation is confirmed, it is possible to screen family members for the disease as part of clinical genetics family counseling.

Once the diagnosis of CGD is established, a genetic analysis may be used to determine exactly which mutation is the underlying cause.

Once more, in other words, Steele is said to be indebted to Swift for his `` wit '' ; ;

Once the scene is set, Trevelyan skilfully builds up the tense story until it reaches its climax in the dramatic victory of Marlborough and Eugene of Savoy at Blenheim.

Once he has been identified, however, a new melody is used to accompany his narrative, a bleak motif with barren octaves creating a rather ancient effect:

Once again what has happened is an all-consuming confabulation of the incidentals, the accidents of painting.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Once more the fallacious equation is advanced to argue that since business is restricted under the anti-monopoly laws, there must be a corresponding restriction against labor unions: the law must treat everybody equally.

One of the finest soft shoe tunes ever invented, `` Once In Love With Amy '' is also, of course, one of the most tantalizingly persistent of light love lyrics to come out of American musical comedy in our era.

Miss Tamiris' `` Once Upon A Time '' is a problem piece about a man and a woman and the three `` figures '' that bother them somehow.

Once a predator has sampled one of these, it is likely to remember the colouration next time it encounters a similar animal.

Once the beams are laid across the building, it is then time to begin the placing of adobe bricks to create the roof.

Once programmed, it is able to choreograph its motions and gestures with its voice.

Once the physical therapist determines that the individual is able to safely perform parts of the program independently, it is important that the individual be prescribed and regularly engage in a supplementary home exercise program that incorporates these components to further improve long term outcomes.

Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.

Once a movement per unit time is observed, astronomers compensate for the parallax caused by the earth ’ s motion during this time and the heliocentric distance to this object is calculated.

Once the anchor is hauled up to the hawsepipe, the ring end is hoisted up to the end of a timber projecting from the bow known as the cathead.

Once the desired scope is laid out, the vessel should be gently forced astern, usually using the auxiliary motor but possibly by backing a sail.

Once again it is important to bear in mind the lack of ' neutral ' state intervention.

Once cytochrome c is released it binds with Apoptotic protease activating factor-1 ( Apaf-1 ) and ATP, which then bind to pro-caspase-9 to create a protein complex known as an apoptosome.

Once when she is, in a key scene, finally invited by the male members of the country club she gives a toast citing from the song “ With rue my heart is laden ”.

Once this complex is formed, it is identified via either a " tag " attached directly to the antibody, or added in an additional technical step.

Once identified, these types of hypoglycemia are readily reversed and prevented, and the underlying disease becomes the primary problem.

Once connected to the access number, the account is identified by keying in a PIN ( the most popular method ) or by swiping a card with embedded chip or magnetic stripe.

Once the nearest object has been identified, the algorithm will estimate the incoming light at the point of intersection, examine the material properties of the object, and combine this information to calculate the final color of the pixel.

Once risks have been identified, they must then be assessed as to their potential severity of impact ( generally a negative impact, such as damage or loss ) and to the probability of occurrence.

Once risks have been identified and assessed, all techniques to manage the risk fall into one or more of these four major categories:

Once a failure mode is identified, it can usually be mitigated by adding extra or redundant equipment to the system.

" Once we have identified the truth predicate's formal features and utility, deflationists argue, we have said all there is to be said about truth.

Once these targets had been identified, they would have been subject to what in essence would have been combined tactical nuclear weapons attacks by the V-Force until they had all been identified and / or destroyed.

Once the target is identified, the missile needs no further guidance during flight ; it is " fire-and-forget ", and the missile operator is free to retreat.

Once the changes have been identified, the replication process makes all the databases look the same.

Once she had identified unreturned items through old inventories, she would write to the holders, requesting that they be returned.

Once targets are identified, candidate drugs can be selected, either from drugs already known to have appropriate effects, or by actually designing the candidate at the molecular level with a computer-aided design program.

Once all decisions have been made, the two groups might be identified as " Group A " and " Group B ".

Once a possible hydrocarbon reservoir is identified, the key physical characteristics of a reservoir that are of interest to a hydrocarbon explorationist are its bulk rock volume, net-to-gross ratio, porosity and permeability.

Once the undesired species in a pasture system are identified an integrated approach of management can be implemented to control weed populations.

Once a market segment has been identified ( via segmentation ), and targeted ( in which the viability of servicing the market intended ), the segment is then subject to positioning.

Once Hepatitis C was identified in 1989, blood banks began screening all blood donors for the presence of the Hepatitis C virus in their bloodstreams.

Once they have identified an invader, the cells generate specific responses that are tailored to maximally eliminate specific pathogens or pathogen infected cells.

Once a candidate for indentured servitude was identified, the emigration agent or visiting ship captain negotiated a binding contract detailing the terms and benefits, and the contract presented before a local magistrate.

Once mutants have been isolated, the mutated gene can be molecularly identified.

Once identified as exudative, additional evaluation is needed to determine the cause of the excess fluid, and pleural fluid amylase, glucose, pH and cell counts are obtained.