Once this complex is formed, it is identified via either a " tag " attached directly to the antibody, or added in an additional technical step.

Once the system had been tested at Highwater, a second barrel was shipped to Foul Bay, attached and strengthened with external bracing to allow it to be raised from the horizontal.

Once it is installed it has become “ attached to the land ” ( the house ) and is now considered real property.

Once the head is attached, the neck and hair are sculpted to properly fit to the figure.

Once that position is filled, advocates believe gender can be attached to the title as appropriate ( chairman or chairwoman ).

Once the film was developed it was sliced down the middle and the ends attached, giving of Standard 8 film from a spool of of 16 mm film.

Once the mooring line is attached to the bollard, it is pulled tight.

Once attached, the island is then known as a tied island.

Once the whale has been killed, a crew member called the " diver " jumps into the water and cuts a hole through the bottom and top of the whale's jaw, to which a tow line and float are attached.

Once the strap is looped around a hand or foot, its attached rope is pulled by rescuers, tightening around the arm or leg and pulling the victim out of the confined space.

Once attached to a substrate, a planula quickly develops into one feeding polyp.

Once within striking distance, the bullfrog emits its feeding strike, which consists of a ballistic lunge ( eyes closed as during all leaps ) that ends with the mouth opening, extension of the fleshy and mucous-coated tongue upon the prey, often engulfing it, while the jaws continue their forward travel to close ( bite ) in close proximity to the prey's original location, just as the tongue is retracted back into the mouth, prey attached.

Once in Britain the division was attached to XVIII Airborne Corps, which commanded all American airborne formations, and which in turn became part of First Allied Airborne Army when it was formed on 21 August under the command of Lieutenant General Lewis H. Brereton.

Once completed, the crew then reenters the Orion, which has been kept attached to the station as an emergency " lifeboat ," seal off the hatches between it and the ISS, and then undock from the station.

Once the tank was filled a plunger attached to a jet nozzle within the tank could be pumped forcing two model dolphins attached to a plastic guide to jump out of the water and through the air.

Once mRNA decoding is complete, the aminoacyl-tRNA is bound in the A / A site and is ready for the next peptide bond to be formed to its attached amino acid.

Once the leaf has been fully formed, the cataphyll usually remains attached where the stem and base of the leaf meet.

Once the breakthrough was achieved, the corps, with the 5th Light Horse Brigade attached, would advance to capture the headquarters of the Ottoman Eighth Army at Tulkarm and the lateral railway line by which the Ottoman Seventh and Eighth Armies were supplied, including the important railway junction at Messudieh.

Once a mesh of a chavetta has been created it is attached to a rig, which is a system of bones which fit inside the chav.

Once in Cape Town the mails were resorted and forwarded to the Field Post Offices attached to formations in the field via the civilian postal services.

Once opened, the attached program would scan the computer for e-mail addresses and e-mail itself to all addresses found.

Once a wagon is filled up, the wagon can be detached and taken back to a silo for unloading, and another wagon can be attached.

Once purchased, software is permanently attached to the user's Steam account ( however " gifting " of games to other accounts is possible ).

Once crossed, the dikaryons are established and a second spore stage is formed, numbered " I " and called aecia, which form dikaryotic aeciospores in dry chains in inverted cup-shaped bodies embedded in host tissue.

Once conditions are favorable, the spore can develop into a new organism using mitotic division, producing a multicellular gametophyte, which eventually goes on to produce gametes.

Once the spores are in a resting state, the single spore must come in contact with a spore of an opposite mating type and of the same species.

Once spore formation is complete, this signifies a mature mushroom and now is able to spread its spores to start a new generation.

Once the pollen grain lands on the stigma of a receptive flower ( or a female cone in gymnosperms ), it takes up water and germinates.

Once a pollen grain settles on a compatible pistil, it germinates in response to a sugary fluid secreted by the mature stigma.

Once the pollen lands on the stigma of a receptive flower, it germinates and starts growing a pollen tube through the carpel.

Once, then -- for how many years or how few does not matter -- my world was bound round by fences, when I was too small to reach the apple tree bough, to twist my knee over it and pull myself up.

Once, Andrus walked by it, hastily scanned the bold black headline and the five-column lead of the article ( by Duane Bosch, staff correspondent -- age not given ), and muttered: `` We a buncha national celebrities ''.

Once she has made up her mind, the queen starts out by constructing, in her chosen abode, a small `` floor '' of dried grass or some woolly material.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Choreographed by Mr. Nagrin, the work filled the second half of a program that also offered the first New York showing of Miss Tamiris' `` Once Upon A Time '' as well as her `` Women's Song '' and Mr. Nagrin's `` Indeterminate Figure ''.

Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.

Once crossed, the soul would be judged by Aeacus, Rhadamanthus and King Minos.

Once a movement per unit time is observed, astronomers compensate for the parallax caused by the earth ’ s motion during this time and the heliocentric distance to this object is calculated.

Once the desired scope is laid out, the vessel should be gently forced astern, usually using the auxiliary motor but possibly by backing a sail.

Once infected by HIV, patients produce antibodies to the more changeable parts of the viral coat.

Once trained and forgotten, completed habits may be activated without feedback sensations that these habits are in effect, just by thinking about them.

Once these projects have been approved by the United States Air Force or other agencies such as the CIA, and are ready to be announced to the public, operations of the aircraft are then moved to a normal air force base.

Once when she is, in a key scene, finally invited by the male members of the country club she gives a toast citing from the song “ With rue my heart is laden ”.

Once a forecasting error is made by agents, due to a stochastic shock, they will be unable to correctly forecast the price level again even if the price level experiences no further shocks since they only ever incorporate part of their errors.

Once this condition is met, it is sufficient that local bonding at the specimen surface is capable of being overcome by the field, allowing for evaporation of an atom from the surface to which it is otherwise bonded.

Once one copy of a document has been produced, printing further copies is often quicker by means of a device called a " thermoform ", which produces copies on soft plastic.

Once, when his younger son Leonid fell seriously ill, Zinaida extracted a promise from her husband, as they stood by the boy's sickbed, that he would end his affair with Olga Ivinskaya.

Once the proposal is accepted by both the creditors and the Court, the debtor makes the payments to the Proposal Administrator each month ( or as otherwise stipulated in their proposal ), and the general creditors are prevented from taking any further legal or collection action.

Once published by BSI a PAS has all the functionality of a British Standard for the purposes of creating schemes such as management systems and product benchmarks as well as codes of practice.

Once the opportunity to claim was presented by management the savers in particular could be relied upon to seize it.

Once the Ionian revolt was finally crushed by the Persian victory at the Battle of Lade, Darius began plans to subjugate Greece.

Once executed, the worm replicates by sending copies of itself to other e-mail addresses found on the host's machine, and installs a keystroke logger, which then captures everything typed on the affected computer.

Once judges began to regard each other's decisions to be binding precedent, the pre-Norman system of local customs and law varying in each locality was replaced by a system that was ( at least in theory, though not always in practice ) common throughout the whole country, hence the name " common law.

Once an idea has been reduced to tangible form, for example by securing it in a fixed medium ( such as a drawing, sheet music, photograph, a videotape, or a computer file ), the copyright holder is entitled to enforce his or her exclusive rights.