Once, then -- for how many years or how few does not matter -- my world was bound round by fences, when I was too small to reach the apple tree bough, to twist my knee over it and pull myself up.

Once bound, β-arrestins both sterically prevent G-protein coupling and may recruit other proteins leading to the creation of signaling complexes involved in extracellular-signal regulated kinase ( ERK ) pathway activation or receptor endocytosis ( internalization ).

Once the ballots are counted and bound together, they are burned in a special stove erected in the Sistine Chapel, with the smoke escaping through a small chimney visible from St. Peter's Square.

Once the men had lured her into the woods, however, they attacked her and knocked her out with chloroform, then stole her purse and bound and gagged her.

Once it was cool enough, the neutrons quickly bound with an equal number of protons to form first deuterium, then helium-4.

Once bound to the prosthetic heme groups, these molecules can modulate the activity / function of those hemeproteins, affording signal transduction.

Once at the lymph nodes, the APC begin to present antigen peptides that are bound to Class II MHC, allowing CD4 < sup >+</ sup > T cells that express the specific TCRs against the peptide / MHC complex to activate.

Once leptin has bound to the Ob-Rb receptor, it activates the stat3, which is phosphorylated and travels to the nucleus to, it is presumed, effect changes in gene expression.

Once the helicase and ATP are bound, local strand separation occurs, which requires binding of ATP but not the actual process of ATP hydrolysis.

Once bound to APC / C, Cdc20 and Cdh1 serve as D and KEN box receptors for various APC substrates.

Once Sar1p is bound to the membrane the coat protein complexes Sec23p / 24p and Sec13p / 31p bind to the membrane sequentially.

Once mRNA decoding is complete, the aminoacyl-tRNA is bound in the A / A site and is ready for the next peptide bond to be formed to its attached amino acid.

Once all the holes are perfected, the bansuri is steeped in a solution of antiseptic oils, after which it is cleaned, dried and its ends are bound with silk or nylon threads for both decoration as well as protection against thermal expansion.

Once a decision is made by the Council, all individual members are bound by it and are obliged to support it publicly.

Once bound, however, antagonists inhibit the function of agonists, inverse agonists, and partial agonists.

Once bound, an antagonist will block agonist binding.

Once the odorant has bound to the odor receptor, the receptor undergoes structural changes and it binds and activates the olfactory-type G protein on the inside of the olfactory receptor neuron.

Once the electron starts to " orbit " the proton, the energy becomes negative, and a bound state – namely the hydrogen atom – is formed.

Once bound, the enzyme is permanently inactive and the cell has to make new enzymes.

Once they had mastered the Drownproofing technique, students learned how to stay afloat with their wrists and ankles bound, swim underwater, and retrieve diving rings from the bottom of the pool using their teeth.

Once a man is bound by marriage, he must be home broken and undergo treatment to assure his fidelity and his leave of nocturnal escapades, a process referred to as domestication ( Papiamento: manza ).

Once the promisee performed completely, consideration is satisfied and a contract is formed and only the promisor is bound to his promise.

Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin ( factor IIa ), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself.

Once the transposase binds to the transposon, it produces a synaptic complex in which two transposases are bound in a cis / trans relationship with the transposon.

Once more, in other words, Steele is said to be indebted to Swift for his `` wit '' ; ;

Once the scene is set, Trevelyan skilfully builds up the tense story until it reaches its climax in the dramatic victory of Marlborough and Eugene of Savoy at Blenheim.

Once he has been identified, however, a new melody is used to accompany his narrative, a bleak motif with barren octaves creating a rather ancient effect:

Once again what has happened is an all-consuming confabulation of the incidentals, the accidents of painting.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Once more the fallacious equation is advanced to argue that since business is restricted under the anti-monopoly laws, there must be a corresponding restriction against labor unions: the law must treat everybody equally.

One of the finest soft shoe tunes ever invented, `` Once In Love With Amy '' is also, of course, one of the most tantalizingly persistent of light love lyrics to come out of American musical comedy in our era.

Miss Tamiris' `` Once Upon A Time '' is a problem piece about a man and a woman and the three `` figures '' that bother them somehow.

Once a predator has sampled one of these, it is likely to remember the colouration next time it encounters a similar animal.

Once the beams are laid across the building, it is then time to begin the placing of adobe bricks to create the roof.

Once programmed, it is able to choreograph its motions and gestures with its voice.

Once the physical therapist determines that the individual is able to safely perform parts of the program independently, it is important that the individual be prescribed and regularly engage in a supplementary home exercise program that incorporates these components to further improve long term outcomes.

Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.

Once a movement per unit time is observed, astronomers compensate for the parallax caused by the earth ’ s motion during this time and the heliocentric distance to this object is calculated.

Once the anchor is hauled up to the hawsepipe, the ring end is hoisted up to the end of a timber projecting from the bow known as the cathead.

Once the desired scope is laid out, the vessel should be gently forced astern, usually using the auxiliary motor but possibly by backing a sail.

Once again it is important to bear in mind the lack of ' neutral ' state intervention.

Once cytochrome c is released it binds with Apoptotic protease activating factor-1 ( Apaf-1 ) and ATP, which then bind to pro-caspase-9 to create a protein complex known as an apoptosome.

Once when she is, in a key scene, finally invited by the male members of the country club she gives a toast citing from the song “ With rue my heart is laden ”.