Once inside the ER, the signal sequence is cleaved from the core protein by signal peptidase.

Once translated, the polyprotein is cleaved by a combination of viral and host proteases to release mature polypeptide products.

Once the stomach content exits the stomach into the duodenum, haptocorrin is cleaved with pancreatic enzymes, releasing the intact vitamin B12.

Once the patient has forced the air into their esophagus, the air vibrates a muscle and creates esophageal voice.

Once again, he inadvertently creates comic mayhem during a show.

Once the front leg creates a perfect 90 degree angle you stand up and alternate legs.

Once filiation has been established, it creates rights and obligations for both the child and the parents, regardless of the circumstances of the child ’ s birth.

Once authorization is acquired, the Originator then creates an ACH entry to be given to an Originating Depository Financial Institution ( ODFI ), which can be any financial institution that does ACH origination.

Once a site is found, the female creates her characteristic S-shaped burrow, laying eggs in the process.

Identifying with nature in this matter necessarily creates a state of mind with a detached objective quality ( Soshitsu Sen 66 ).” Once a tea grower invited Rikyū to have tea.

Once the adhesive cures, it creates a very strong bond between grip and shaft and the grip is usually impossible to remove without cutting it off.

Once there, Tomcat ( aka Darovit ) creates an agreement between Zannah and Caleb ( the healer who helped Bane in the previous novel ) designed to ensure their survival.

Once the player has finished giving orders for the turn, either the client program or an external helper program takes the data, creates a turn file ( usually with a. TRN extension ), and sends it to the host person, who feeds all the turn files into the host program.

Once all the minigames are completed, the game creates a slideshow or video of the player doing the tasks, usually speeding them up, or freezing them into a picture.

Once Palpatine creates the Galactic Empire with himself as Emperor, the Royal Guards accompany him at all times.

Once a student completes a computer composition, they must " bounce " their Pro Tools file to disk, which creates a single AIFF file ready to be burned to CD.

Once a player creates a missionary, he or she can send it to another city to spread that missionary's religion.

Once emplaced and anchored with sutures, the surgeon then creates a pinna ( outer ear ) of natural proportions, contour, and appearance.

Once the Enterprise is in orbit about the planet, he creates a programmed sequence of force fields to allow him to move from the bridge to the nearest transporter room without being stopped by security, and then beams down to the planet, leaving the Enterprise still under his lockdown.

Once installed on a computer, the trojan creates two registry keys: one to ensure it is run on every system startup, and the second to monitor the progress of the trojan in the infected computer, counting the number of files that have been analyzed by the malicious code.

Once sufficiently clear of hairs, the wasp creates a bare patch on the larva ’ s abdomen and lays 2 – 4 eggs.

Once the anchor is hauled up to the hawsepipe, the ring end is hoisted up to the end of a timber projecting from the bow known as the cathead.

Once the called party answered, the local operator would advise him or her to stand by for the calling party, whom she'd then ring back.

Once he shone on his own, now he sits home alone and waits for the phone to ring.

* Once the bell rings the rider is allowed 45 seconds to enter the ring or is eliminated.

Once considered the world's fastest man by virtue of his multiple world records in the 60-yard, 100-yard, 220-yard, and Olympic 100-meter dashes, Hayes is the only man to win both an Olympic gold medal and a Super Bowl ring.

Once the piercing is healed, it is possible to wear a captive bead ring in it, although depending on the placement of the piercing, a D-ring styled ring may be necessary to prevent migration caused by the pressure exerted by the shape of a ring.

Once that is done, the theorem is proven for abelian groups or modules over a ring.

Once the engine starts, the key-operated switch is opened, a spring in the solenoid assembly pulls the pinion gear away from the ring gear, and the starter motor stops.

Once the engine starts and the driver releases the starter switch, a spring retracts the pole shoe, which pulls the starter drive out of engagement with the ring gear.

Once the outside of the tent is raised a crew of men can go under the tent and start raising the bail ring up the center pole using a system of pulleys.

Once the third-ranked heavyweight contender, Henry was released after posting $ 2, 000 bail and subsequently retired from the ring.

Once a certain distance has been excavated ( roughly 1. 5-2 meters ), a new tunnel ring is built using the erector.

Once wearing the ring and raising his fist, the ring is struck by flashes of lightning ( referred to as the Thunder of Zeus in several episodes ).

Once the ring-bearer's resistance reaches zero, he is corrupted by the power of the ring and the player is eliminated from the game.

Once the two spacecraft were locked together, the docking ring on the Orbiter Docking System retracted, and a series of hooks and latches were engaged to ensure an airtight seal between the two spacecraft.

Once the A45 leaves Daventry for Coventry, the remaining section of single carriageway ring road is signed as A361, before it once again turns north to Kilsby to terminate on the A5 at its northern end.

Once the stations had developed sufficient inward velocity the ring current would be reactivated and the resulting magnetic field would be used to reverse the stations ' fall.

Once in the storage ring, the electrons produce x-ray beams that are available for use in experimentation.

Once the innermost ring has been breached, the central weapon is vulnerable to attack from the player.

Once there, Gemini agrees to operate to remove the ring from Emilio in exchange for a year of experimentation rights on Wolfina.

Once a tag is made, the wrestler tagging out has a grace period ( typically five to ten seconds ) to leave the ring before risking disqualification.

Once Hal Jordan recharged his Power Ring, he helped Batman fight Sinestro where Sinestro was disarmed of his ring by Batman ( who ironically opened his hand with Sinestro's power ring in it ) who then knocked out Sinestro.