Once the PMCs disrupt all attachment to their former location, the cells themselves change their morphology by contracting their apical surfaces, apical constriction, and enlarging their basal surfaces, thus acquiring a “ bottle cell ” phenotype.

Once destruction of the brain is complete, the cerebellum, midbrain, thalami, basal ganglia, choroid plexus, and portions of the occipital lobes typically remain preserved to varying degrees.

Once elevated, the intracellular calcium concentration is returned to its basal level through a variety of protein pumps and calcium exchangers located on the plasma membrane and sarcoplasmic reticulum.

Once the rate of rain fall is faster than the rate of infiltration into the soil, surface runoff occurs and carries the loosened soil particles down slope.

Once this improved sensor was available, Geiger counter instruments could be produced relatively cheaply because the large output pulse required little electronic processing to give a count rate reading, which was a distinct advantage in the thermionic valve era due to valve cost and power consumption.

Once that relatively low data limit is reached the customer has to recharge the service using a prepaid card at a rate of approximately 10 u $ s / GB.

Once the velocity field is solved for, other quantities of interest ( such as flow rate or drag force ) may be found.

Once that rate has been reached, applying greater pedal pressure will not result in a change of this rate, and indeed the effects mentioned can substantially reduce it.

Once a car's suspension reaches the bump stops, its effective spring rate increases sharply as the bump stops are effectively very hard springs.

Once started, it usually rolls in a clockwise direction at a rate of about 12 m / s ( 40 ft / s ), or about 22 seats per second.

' Once volley firing had been developed, rate of fire and efficiency had increased and the arquebus became the most effective tool available to the late 16th century armies that carried them.

Once the notion that the universe started with a rapid inflation nicknamed the Big Bang became accepted by the majority of scientists, the ultimate fate of the universe became a valid cosmological question, one depending upon the physical properties of the mass / energy in the universe, its average density, and the rate of expansion.

Once again this was viewed as a demotion, however, O ' Kennedy viewed his role as one of the most important in the cabinet considering the high rate of unemployment.

Once the production rate falls off, the well is put through another cycle of injection, soak and production.

Once the initial respiratory and hemodynamic goals have been achieved, the blood flow is maintained at that rate.

Once the critical shear stress ( or " yield stress ") is exceeded, the material flows in such a way that the shear rate, ∂ u /∂ y ( as defined in the article on viscosity ), is directly proportional to the amount by which the applied shear stress exceeds the yield stress:

Once the temperature reaches 100 million – 200 million kelvins and helium fusion begins using the triple-alpha process, the temperature rapidly increases, further raising the helium fusion rate and, because degenerate matter is a good conductor of heat, widening the reaction region.

Once bacterial slime starts to grow, the corrosion rate of the system increases.

Once they had witnessed nearly a doubling of the rate of LP activation, they knew this belief in that keeping cells intact was crucial to studying the effects of hormones was not necessarily true, at least in this case.

Once rupture occurs, the mortality rate is 50 – 80 %, and most deaths in patients with the Marfan syndrome are the result of aortic disease.

Once the exterior has started to crust, then the rate of rotation is adjusted down so as to minimize dripping.

Once the memory was filled ( usually only a few seconds maximum ), the unit would then replay the recording at slower rate, typically between 1 / 10 to 1 / 32 of the rate of the original recording.

Once the monkey was fully trained, there was no increase in firing rate upon presentation of the predicted reward.

Once the pricing, or the initial spread over a base rate which is usually LIBOR, was set, it was set, except in the most extreme cases.

Once more, in other words, Steele is said to be indebted to Swift for his `` wit '' ; ;

Once the scene is set, Trevelyan skilfully builds up the tense story until it reaches its climax in the dramatic victory of Marlborough and Eugene of Savoy at Blenheim.

Once he has been identified, however, a new melody is used to accompany his narrative, a bleak motif with barren octaves creating a rather ancient effect:

Once again what has happened is an all-consuming confabulation of the incidentals, the accidents of painting.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Once more the fallacious equation is advanced to argue that since business is restricted under the anti-monopoly laws, there must be a corresponding restriction against labor unions: the law must treat everybody equally.

One of the finest soft shoe tunes ever invented, `` Once In Love With Amy '' is also, of course, one of the most tantalizingly persistent of light love lyrics to come out of American musical comedy in our era.

Miss Tamiris' `` Once Upon A Time '' is a problem piece about a man and a woman and the three `` figures '' that bother them somehow.

Once a predator has sampled one of these, it is likely to remember the colouration next time it encounters a similar animal.

Once the beams are laid across the building, it is then time to begin the placing of adobe bricks to create the roof.

Once programmed, it is able to choreograph its motions and gestures with its voice.

Once the physical therapist determines that the individual is able to safely perform parts of the program independently, it is important that the individual be prescribed and regularly engage in a supplementary home exercise program that incorporates these components to further improve long term outcomes.

Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.

Once a movement per unit time is observed, astronomers compensate for the parallax caused by the earth ’ s motion during this time and the heliocentric distance to this object is calculated.

Once the anchor is hauled up to the hawsepipe, the ring end is hoisted up to the end of a timber projecting from the bow known as the cathead.

Once the desired scope is laid out, the vessel should be gently forced astern, usually using the auxiliary motor but possibly by backing a sail.

Once again it is important to bear in mind the lack of ' neutral ' state intervention.

Once cytochrome c is released it binds with Apoptotic protease activating factor-1 ( Apaf-1 ) and ATP, which then bind to pro-caspase-9 to create a protein complex known as an apoptosome.

Once when she is, in a key scene, finally invited by the male members of the country club she gives a toast citing from the song “ With rue my heart is laden ”.