Once crossed, the dikaryons are established and a second spore stage is formed, numbered " I " and called aecia, which form dikaryotic aeciospores in dry chains in inverted cup-shaped bodies embedded in host tissue.

Once other investigations have indicated Wilson's disease, the ideal test is the removal of a small amount of liver tissue through a liver biopsy.

) David Gollaher has written that the rabbis added the procedure of periah to discourage men from trying to restore their foreskins: ‘ Once established, periah was deemed essential to circumcision ; if the mohel failed to cut away enough tissue, the operation was deemed insufficient to comply with God's covenant ’ and ‘ Depending on the strictness of individual rabbis, boys ( or men thought to have been inadequately cut ) were subjected to additional operations .’ In addition to milah ( the actual circumcision ) and priah, mentioned above, the Talmud mentions a third step, metzitzah, or squeezing some blood from the wound.

Once ingested, microcystin travels to the liver, via the bile acid transport system, where most is stored ; though some remains in the blood stream and may contaminate tissue.

Once formed, cGMP can be degraded by phosphodiesterases, which themselves are under different forms of regulation, depending on the tissue.

Once the tissue is removed, it is sent to tissue banks for processing and distribution.

Once in the anterior chamber and while firmly holding the lens with forceps, temporal and nasal iris tissue is enclavated with a special needle.

Once acorns sprout, they are less nutritious, as the seed tissue converts to the indigestible lignins that form the root.

Once healed, transscrotal piercings can be stretched quickly and, given the amount of surrounding tissue, to great size if desired.

Once the fatty acids reach the target tissue, they are released by serum albumin and cross into the cytosol.

Once dendritic cells arrive in secondary lymphoid tissue, however, they lose these properties while gaining the capacity to interact with naive T-cells.

Once the plague wiped out her village, she discovered that not only had she survived, she had somehow mutated ; she no longer carried the gene for aging, she was immune to disease, and her body could recover tissue damage at roughly 700 % of normal based on Dr. Sinclair's estimates.

Once the solid substrate bead technology has been chosen, antibodies are coupled to the beads and the antibody-coated-beads can be added to the heterogeneous protein sample ( e. g. homogenized tissue ).

Once the plant material is chosen for culture, the collection of explant ( s ) begins and is dependent on the type of tissue to be used ; including stem tips, anthers, petals, pollen and others plant tissues.

Once in the lung or ectopic site, the worm stimulates an inflammatory response that allows it to cover itself in granulation tissue forming a capsule.

Once inserted into the tissue or ( body ) fluid of interest, small solutes can cross the semipermeable membrane by passive diffusion.

Once the tissue is set in an altered form, Kro has only to stop concentrating and his body will retain that shape until such time as he concentrates upon changing it again.

Once more, in other words, Steele is said to be indebted to Swift for his `` wit '' ; ;

Once the scene is set, Trevelyan skilfully builds up the tense story until it reaches its climax in the dramatic victory of Marlborough and Eugene of Savoy at Blenheim.

Once he has been identified, however, a new melody is used to accompany his narrative, a bleak motif with barren octaves creating a rather ancient effect:

Once again what has happened is an all-consuming confabulation of the incidentals, the accidents of painting.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Once more the fallacious equation is advanced to argue that since business is restricted under the anti-monopoly laws, there must be a corresponding restriction against labor unions: the law must treat everybody equally.

One of the finest soft shoe tunes ever invented, `` Once In Love With Amy '' is also, of course, one of the most tantalizingly persistent of light love lyrics to come out of American musical comedy in our era.

Miss Tamiris' `` Once Upon A Time '' is a problem piece about a man and a woman and the three `` figures '' that bother them somehow.

Once a predator has sampled one of these, it is likely to remember the colouration next time it encounters a similar animal.

Once the beams are laid across the building, it is then time to begin the placing of adobe bricks to create the roof.

Once programmed, it is able to choreograph its motions and gestures with its voice.

Once the physical therapist determines that the individual is able to safely perform parts of the program independently, it is important that the individual be prescribed and regularly engage in a supplementary home exercise program that incorporates these components to further improve long term outcomes.

Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.

Once a movement per unit time is observed, astronomers compensate for the parallax caused by the earth ’ s motion during this time and the heliocentric distance to this object is calculated.

Once the anchor is hauled up to the hawsepipe, the ring end is hoisted up to the end of a timber projecting from the bow known as the cathead.

Once the desired scope is laid out, the vessel should be gently forced astern, usually using the auxiliary motor but possibly by backing a sail.

Once again it is important to bear in mind the lack of ' neutral ' state intervention.

Once cytochrome c is released it binds with Apoptotic protease activating factor-1 ( Apaf-1 ) and ATP, which then bind to pro-caspase-9 to create a protein complex known as an apoptosome.

Once when she is, in a key scene, finally invited by the male members of the country club she gives a toast citing from the song “ With rue my heart is laden ”.

Once the divergences between all pairs of samples have been determined, the resulting triangular matrix of differences is submitted to some form of statistical cluster analysis, and the resulting dendrogram is examined in order to see whether the samples cluster in the way that would be expected from current ideas about the taxonomy of the group, or not.

Once each player has examined his or her cards and placed them face down on the layout, they may not touch the cards again.

Once samples have been prepared chemically, they are mounted on microscope slides using silicon oil, glycerol or glycerol-jelly and examined using light microscopy or scanning electron microscopy.

Once the external evidence is collected, the body is removed from the bag, undressed, and any wounds present are examined.

Once at the exchange point, the outer envelope was removed and discarded while the inner cover containing the prisoner's letter was examined by military officials and delivered.

Once crystallized, the sample remains in the furnace to be examined post-flight.

Once all of the witnesses have been examined, the trial moves to closing arguments.

Although there is no central controlled vocabulary to constrain the actions of individual users, the distributions of tags that describe different resources have been shown to converge over time to stable power law distributions .. Once such stable distributions form, the correlations between different tags can be examined to construct simple folksonomy graphs, which can be efficiently partitioned to obtain a form of community or shared vocabularies.

Once more, applicants ' credit-worthiness had to be examined.

Once a trademark application has been examined by a USPTO examining attorney and is found to be entitled to registration, it is published in the Official Gazette of the USPTO.

Once there, the bones were examined by experts from both the US and Europe.

Once Wang Mingdao's strict entry requirements have already been satisfied, both the new convert ’ s behaviour and lifestyle will be closely observed or examined by church members for a period.