Once all guests have told their stories, there is usually a " question and answer " segment where audience members ask guests questions relevant to their situations, although usually their questions come to insult a guest or they flash the audience in exchange for " Jerry Beads " ( Mardi Gras-style beads with the show logo ).

Once this has been added, the cell is ruptured and its cell contents are exposed to synthetic beads that are coated with thymine string nucleotides.

Once the wire cools, the rings would be slipped off and then used as beads.

Once the solid substrate bead technology has been chosen, antibodies are coupled to the beads and the antibody-coated-beads can be added to the heterogeneous protein sample ( e. g. homogenized tissue ).

Once this has occurred the immunoprecipitation portion of the protocol is actually complete, as the specific proteins of interest are bound to the antibodies that are themselves immobilized to the beads.

Once finished, the beads or knots of the witch's ladder enable a witch to concentrate on repetitive chants or incantation without having to keep count.

Once the piercing is fully healed, one of the beads at the end of the surface bar can be replaced with a special bead, drilled to accept a ring, similar to the bead on a bondage bar.

Once the poetic arts are separated from the other forms, he lays down his famous definition of tragedy, which sets up standards and so lends direction to the remainder of the work.

Once we have made clear that we are genuinely concerned with a country's development potential, we can be blunt in suggesting the technical conditions that must be met for development to occur.

Once these conditions of international law are met, countries that try to run the blockade do so at their own risk.

Once many significant phrases are found in theory or in recurrent practice to provide for prosodic necessity, they are not to be defended for their semantic properties in isolated contexts.

Once the grains are ground, vitamin E begins to deteriorate immediately and half of it is lost by oxidation and exposure to the air within one week.

Once the beams are laid across the building, it is then time to begin the placing of adobe bricks to create the roof.

Once he has received this blessing, the abbot not only becomes father of his monks in a spiritual sense, but their major superior under canon law, and has the additional authority to confer the ministries of acolyte and lector ( formerly, he could confer the minor orders, which are not sacraments, that these ministries have replaced ).

Once the game ends, the minority and majority bonuses are paid to the minority and majority holders in each of the remaining chains ; each player sells his or her shares of stock in each of the remaining chains ; and the player with the most money wins.

Once trained and forgotten, completed habits may be activated without feedback sensations that these habits are in effect, just by thinking about them.

Once these projects have been approved by the United States Air Force or other agencies such as the CIA, and are ready to be announced to the public, operations of the aircraft are then moved to a normal air force base.

Once the adhesive and the adherend are in close proximity, molecular interactions, such as van der Waals forces, become involved in the bond, contributing significantly to its ultimate strength.

Once the surfaces are pushed together, the bond forms very quickly.

Once past the South Gate, the Cologne-Mainz road continued along what are now streets named Belderberg, Adenauerallee et al.

Once the proposal is accepted by both the creditors and the Court, the debtor makes the payments to the Proposal Administrator each month ( or as otherwise stipulated in their proposal ), and the general creditors are prevented from taking any further legal or collection action.

Once a largely agricultural country, the twin pillars of Costa Rica's current economy are technology and eco-tourism.

Once the prion is transmitted, the defective proteins invade the brain and are produced in a self-sustaining feedback loop.

Once a person in a coma is stable, investigations are performed to assess the underlying cause.

Once adsorbed, the bonds within the reacting molecules are weakened, and new bonds between the resulting fragments form in part due to their close proximity.

Once the electronic and nuclear variables are separated ( within the Born – Oppenheimer representation ), in the time-dependent approach, the wave packet corresponding to the nuclear degrees of freedom is propagated via the time evolution operator ( physics ) associated to the time-dependent Schrödinger equation ( for the full molecular Hamiltonian ).

Once accepted, these are finished by filling them with very-low-density concrete.

Once the x-and y-axes are specified, they determine the line along which the z-axis should lie, but there are two possible directions on this line.

Once begun, avalanches are often intensified by the creation of photoelectrons as a result of ultraviolet radiation emitted by the excited medium's atoms in the aft-tip region.

Once the empty line is inserted in line 2, the line which reads " This is line number two.

Once carefully inserted into the socket of the arrowhead, the tongs screwed apart till they gripped its walls and allowed the head to be extracted from the wound.

Once inserted, the operational balloon would have a total mass of no more than and would remain operational for a week.

Once implanted, the port is accessed via non-coring " Huber " needles inserted through the skin.

Once the arms are inserted into the straitjacket's sleeves, they are then crossed across the chest.

Once inserted into a TEM, the sample often has to be manipulated to present the region of interest to the beam, such as in single grain diffraction, in a specific orientation.

Once these pseudogenes are inserted back into the genome, they usually contain a poly-A tail, and usually have had their introns spliced out ; these are both hallmark features of cDNAs.

Once the applicators are inserted, they are held in place against the skin using sutures or adhesive tape to prevent them from moving.

Once it is inserted, a bladder at the tip of the airway is inflated, to block (" obturate ") the esophagus, and oxygen is delivered through a series of holes in the side of the tube which is then forced into the lungs.

Once the instrument was properly adjusted, a photographic plate was inserted and exposed.

Once the tube is past the pharynx and enters the esophagus, it is easily inserted down into the stomach.

Once it has been decided that ECMO will be initiated, the patient is anticoagulated with intravenous heparin and then the cannulae are inserted.

Once membrane-targeting is completed, the signal sequence is inserted into the translocon.

Once the magazine is empty, the slide will lock back, released only when the slide stop is depressed ; if a new magazine is inserted before the slide stop is depressed then a new cartridge will be chambered.

Once AMPARs are inserted, the synapse is no longer silent ; activated synapses no longer require simultaneous pre-and postsynaptic activity in order to elicit EPSPs.

Once ready, a cork stopper is inserted to block one end, next to which the blowing hole is burnt in.

It offers a refrigerated Automated Convenience Store containing up to 200 products at 34 degrees F. Once cash or Campus card is inserted and products are picked, a robot will fetch and deliver your product.

Once an audience assembled, the storyteller told several stories using a set of illustrated boards, inserted into the stage and withdrawn one by one as the story was told.

Once the temperature has stabilized the sample is inserted.

Once your method is inserted, RubyCocoa will retrieve the signature of the existing Objective-C method and inject a new one to the Objective-C runtime, of the same signature, but which now points to your code.

Once inserted the lens is fastened by turning it a small amount.

Once inserted into any of these areas, the magnets allow the Stikfas to be posed while adhered to a magnetic surface so they won't fall over.

Once a breach in the enemy tactical position was made, more mobile units such as tank and mechanized corps would be inserted through the Shock Army's positions with the mission of penetrating deep into the enemy rear area.

Once the ostium is identified the falloposcope can be inserted through the operative channel of the hysteroscope and advanced.