Once hybridisation occurs in situ, RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies that are conjugated to alkaline phosphatase.

Once the rate of rain fall is faster than the rate of infiltration into the soil, surface runoff occurs and carries the loosened soil particles down slope.

Once on Ireland but outside the south west there are two confirmed four-posters, both on the east coast, suggesting a route down that side of the island, to the region around County Cork, where another proliferation occurs.

Once this area is activated and the recognition of dreaming occurs, the dreamer must be cautious to let the dream continue but be conscious enough to remember that it is a dream.

# Once structural diversity is established anatomically, a second selective process occurs during postnatal behavioral experience through epigenetic modifications in the strength of synaptic connections between neuronal groups.

Once this occurs, the core reaches hydrostatic equilibrium: the electron degeneracy pressure is sufficient to balance gravitational pressure.

Once this occurs, the effect is dramatic, and the dust devil dissipates in seconds.

Once at this stage, the eggs embryonate and then become infective, a process that occurs in about 15 to 30 days ).

Once this obstruction occurs, the appendix subsequently becomes filled with mucus and swells, increasing pressures within the lumen and the walls of the appendix, resulting in thrombosis and occlusion of the small vessels, and stasis of lymphatic flow.

Once the Jury starts to form, the members are present at every Tribal Council, but are not allowed to speak or interact with the players still in the game ; they are only there to observe the questioning and voting that occurs.

Once innovation occurs, innovations may be spread from the innovator to other individuals and groups.

Once this occurs, the aircraft will climb to a higher altitude until the lifting force and weight are again in balance.

# Once an individual has been interred, microbial attack, the most common mechanism of bone deterioration, occurs rapidly.

Once this occurs, the bus rises up into a cloud along with evaporating water.

Once invasion occurs, they are no longer considered CIS lesions, but truly invasive carcinomas.

Once a fire occurs it can spread fast due to the summer heat that has dry grass, bushes and trees.

Once this immunological impairment occurs, the tonsil is no longer able to function adequately in local protection nor can it appropriately reinforce the secretory immune system of the upper respiratory tract.

Once nilas has formed, a quite different growth process occurs, in which water molecules freeze on to the bottom of the existing ice sheet, a process called congelation growth.

Once all the creatures are eliminated, an earthquake occurs and the planet's lava levels decrease, allowing Samus to travel deeper through its tunnels.

Once air enters the lungs, diffusion of O < sub > 2 </ sub > and CO < sub > 2 </ sub > occurs in the alveoli.

Once the mast cell-antibody-antigen complex is formed, a complex series of events that eventually leads to cell degranulation and the release of histamine ( and other chemical mediators ) from the mast cell or basophil occurs.

Once the two meet, a calcium influx occurs, causing a signaling cascade.

Once the helicase and ATP are bound, local strand separation occurs, which requires binding of ATP but not the actual process of ATP hydrolysis.

Once this occurs "… ritual turns a meaningful idea into a visceral experience.

Once he discovered their true intent, to use the mold's corrosive properties to destroy America and make a comeback, he assembled a list of Nazi agents, the " Friends of Carlotta ".

Once fastened, the Scraplets release some kind of corrosive agent that both allows them to sink partly underneath the armour plating and make them impossible to remove.

Once this post-translational modification process has been completed, the protein begins to fold ( sometimes spontaneously and sometimes with enzymatic assistance ), curling up on itself so that hydrophobic elements of the protein are buried deep inside the structure and hydrophilic elements end up on the outside.

Once struck, the flame from the cap in turn ignited the main charge of gunpowder, as with the flintlock, but there was no longer any need to charge the touch hole with gunpowder, and even better, the touch hole was no longer exposed to the elements.

Once again, Pei sought to combine new design elements with the classical aesthetic most appropriate for the location of the building.

Once these religious elements are removed, Richards argues that it is clear " the whole episode was political in nature.

Once the hydrogen fuel at the core is exhausted, a star with at least 0. 4 times the mass of the Sun expands to become a red giant, in some cases fusing heavier elements at the core or in shells around the core.

Once data elements are discovered they can be registered in a metadata registry.

Once we've visited every element, we will no longer be able to remove any element without changing the answer from true to false ; at this point the remaining subset of the original elements must sum to zero.

Once the panels are sewn together ( often by triple-stitch method ), the sailmakers complete the sail by placing the finishing elements such as the leech and foot lines, protective patches in the areas where the sail will scrape against hardware ( stanchions, spreaders ), steel rings and straps at the tack and clew, cleats, batten pockets ( if required ) and sail numbers.

Once a peace treaty has been negotiated, the parties involved might ask the United Nations for a peacekeeping force to oversee various elements of the agreed upon plan.

Once the logic is complete for all elements in the FMECA, the resulting list of maintenance is " packaged ", so that the periodicities of the tasks are rationalised to be called up in work packages ; it is important not to destroy the applicability of maintenance in this phase.

Another concept album, also released in 1977, was Once Upon a Time, a double album which told of a modern-day Cinderella " rags to riches " story through the elements of orchestral disco and ballads.

Once the fuel elements of a reactor begin to melt, the primary containment has been breached, and the nuclear fuel ( such as uranium, plutonium, or thorium ) and fission products ( such as cesium-137, krypton-88, or iodine-131 ) within the fuel elements can leach out into the coolant.

Once the axioms were clarified ( that inverse elements should be required, for example ), the subject could proceed autonomously, without reference to the transformation group origins of those studies.

Once the movement had begun, events moved rapidly — not always fast enough to satisfy the nationalist leaders, but still at a pace that surprised not only the colonial government but many of the more conservative African elements as well.

Once that change is made, all of the 0. 01 elements will be added, giving 0. 99, and then the 1. 0 element will be added, yielding a rounded result of 2. 0 – a much better approximation of the real result.

* While Warner Bros. and Turner hold the film elements of Hanna-Barbera's Once Upon a Forest, all theatrical, television and home entertainment rights are completely and exclusively controlled by 20th Century Fox.

" Once on location, the ghillie suit is customized with twigs, leaves, and other elements of the local foliage as much as possible, although these local additions must be changed every few hours, due to wilting of green grasses or branches.

Once both of these two elements manifest themselves, the bullying cycle often proceeds to feed on itself over time, and may last for months, years, or even decades.

Once a sufficient number of the atoms which constituted the molecule were annihilated by the antimatter, the molecule could not remain stable, and thus degenerated into a selection of less complex compounds and elements, effectively causing the hull to vanish in an instant.

Once the two elements were docked, Ross and Newman conducted two scheduled spacewalks to connect power and data cables between the Node, PMAs and the FGB.

Once he makes a final decision on a layout, he will then print it out at full size, and use a light box to pencil it, sometimes altering elements in the design such as lighting or other details.

Once he makes a final decision on a layout, he will then print it out at full size, and use a light box to pencil it, sometimes altering elements in the design such as lighting or other details.