Once a character is created, it can only be played on that server unless the character is transferred to a new server by the customer service staff, generally for a fee.

Once the RNA has been transferred to the membrane, it is immobilized through covalent linkage to the membrane by UV light or heat.

Once they had proven themselves trustworthy, they would be transferred to open dorms within the compound.

Once the heat has been transferred and the water has cooled, the water is re-injected back into the ground.

Once found, the player that possesses an idol may keep it or transfer it to another player before or during Tribal Council ( in Cook Islands, it couldn't be transferred during tribal council ), and it cannot be stolen from that player.

Once East Pawtucket ( another alternate name for the area of Pawtucket east of the Blackstone ) was transferred to Rhode Island in the 1860s, both sections ( West and East Pawtucket ) merged.

Once this initial energy is added, the wave travels through the medium until all its energy is transferred.

" Once the meaning had transferred from the substance to the shape, piggy banks began to be made from other substances, including glass, plaster, and plastic.

Once their usage period is expired, documents are transferred to archives, where they are either destroyed ( by incineration, crushing or electrical overtension ), or stored.

Once primary fermentation is complete, the beer is either transferred to a secondary carboy for conditioning or it can be transferred directly to bottles for conditioning.

Once the proteins are well-separated, they are transferred to a membrane and the procedure continues similar to an ELISA: the person's diluted serum is applied to the membrane and antibodies in the serum may attach to some of the HIV proteins.

Once the mortgage had been fully repaid, the Wintertons transferred the ownership of the flat into a family trust.

Once the work is lawfully sold or even transferred gratuitously, the copyright owner's interest in the material object in which the copyrighted work is embodied is exhausted.

Once in protected mode, the virtual device drivers ( VxDs ) transferred all state information from MS-DOS to the 32-bit file system manager, and then shut off MS-DOS.

Once CSNET was fully operational, the systems and ongoing network operations were transferred to Bolt Beranek and Newman ( BBN ) of Cambridge, Massachusetts by 1984.

Once a congregation had an angel and the fourfold ministry from local people ( not including ministers who had transferred from other congregations ), the full services could be held.

Once cattle obtain an entry-level weight, about 650 pounds ( 300 kg ), they are transferred to a feedlot to be fed a specialized diet which consists of corn byproducts ( derived from ethanol production ), barley, and other grains as well as alfalfa, cottonseed meal, and minerals.

Once settled as an autonomous school, became part of Tohoku Imperial University in 1907, and was transferred to Hokkaido Imperial University ( current Hokkaido University ) in 1918.

Once land was a part of the U. S. public domain, it was thereafter held in allodial title, meaning that it could not be alienated from federal authority, but might be removed from the public domain ( as in the creation of a new state ) or transferred and owned in fee simple.

Once eligibility and level of benefits have been determined, information is transferred to the state's EBT contractor and an account is established in the participant's name, and SNAP benefits are deposited electronically in the account each month.

" Once earned, lead jammer status cannot be transferred to other skaters, but certain actions ( notably, being sent to the penalty box ) can cause it to be lost.

Once mature and adopted by the broader community, the standards are usually transferred to the IEEE.

Once the United States entered World War II, Johnston transferred to the U. S. Army Air Corps Ferry Command.

Once this voltage is exceeded, the material ceases being an insulator, and charge will begin to pass through it.

Once these conditions of international law are met, countries that try to run the blockade do so at their own risk.

Once on the water, these little visitors seldom leave, and this adds to your filtering and vacuuming problems as well as providing a slapping good time for all those present.

Once he disposed of these items which screamed so harshly for attention, he could undertake the things which really counted.

Once a predator has sampled one of these, it is likely to remember the colouration next time it encounters a similar animal.

Once the physical therapist determines that the individual is able to safely perform parts of the program independently, it is important that the individual be prescribed and regularly engage in a supplementary home exercise program that incorporates these components to further improve long term outcomes.

Once he has received this blessing, the abbot not only becomes father of his monks in a spiritual sense, but their major superior under canon law, and has the additional authority to confer the ministries of acolyte and lector ( formerly, he could confer the minor orders, which are not sacraments, that these ministries have replaced ).

Once trained and forgotten, completed habits may be activated without feedback sensations that these habits are in effect, just by thinking about them.

Once these projects have been approved by the United States Air Force or other agencies such as the CIA, and are ready to be announced to the public, operations of the aircraft are then moved to a normal air force base.

Once players have mastered these basic strokes, they can hit the shuttlecock from and to any part of the court, powerfully and softly as required.

Once informed of the accusations of these " lewd rustics ," Bede refuted them in his Letter to Plegwin.

Once accepted, these are finished by filling them with very-low-density concrete.

Once these basic shapes have been assembled into a likeness, then the drawing can be refined into a more accurate and polished form.

Once these laws were passed, Finnish authorities signed the United Nations Convention on the Elimination of All Forms of Discrimination Against Women, in 1986.

Once in office, these individuals maintain direct contact with the Queen, wherever she may be at the time.

Once a metamorphic rock is melted it is no longer a metamorphic rock and is a magma, so these rocks are seen as a transitional between the two, but are not technically granite as they do not actually intrude into other rocks.

Once the individual is awake they may feel tired, irritable, or confused and these may be signs of hypoglycemia as well.

Once identified, these types of hypoglycemia are readily reversed and prevented, and the underlying disease becomes the primary problem.

Once these skills are acquired the reader can attain full language literacy, which includes the abilities to approach printed material with critical analysis, inference and synthesis ; to write with accuracy and coherence ; and to use information and insights from text as the basis for informed decisions and creative thought.

On Chomsky specifically, he writes that " imaginary problems were created by the whole series of dichotomies that Chomsky introduced, or took over unproblematized: not only syntax / semantics but also grammar / lexis, language / thought, competence / performance ... Once these dichotomies had been set up, the problem arose of locating and maintaining the boundaries between them linguistics "

Once these support and requisite conditions have been established, a practitioner can then practice right concentration more easily.

Once these religious elements are removed, Richards argues that it is clear " the whole episode was political in nature.

During April 2009, U. S. Federal Reserve Vice Chair Janet Yellen discussed these paradoxes: " Once this massive credit crunch hit, it didn ’ t take long before we were in a recession.

Once this was understood, the various reign lengths and cross-synchronisms for these kings was worked out, and the sum of reigns for both kingdoms produced 931 / 930 BC for the division of the kingdom when working backwards from the Battle of Qarqar in 853 BC.

Once risks have been identified and assessed, all techniques to manage the risk fall into one or more of these four major categories:

Once activated, these exchange factors can activate more small G proteins, thus amplifying the receptor's initial signal.