In all cases a disturbing amount of nonspecific staining was still present although it was still distinguishable from specific fluorescence.

Most fluorescence microscopes are operated in the Epi-illumination mode ( illumination and detection from one side of the sample ) to further decrease the amount of excitation light entering the detector.

It seems to have a slight amount of fluorescence.

Alumina aerogels are also being considered by NASA for capturing of hypervelocity particles ; a formulation doped with gadolinium and terbium could fluoresce at the particle impact site, with amount of fluorescence dependent on impact energy.

The amount of fluorescence visualized can be used as a quantitative measure of the amount of filamentous actin there is in cells if saturating quantities of fluorescent phalloidin are used.

The intensity of fluorescence of a spot is proportional to the amount of target sequence that has hybridized to that spot, and therefore to the abundance of that mRNA sequence in the sample.

Micelles can imbibe and hold a considerable amount of oleophilic substances so that the micelle volume may be increased as much as approximately two-fold.

The length of the arrow indicates amount of slowing or acceleration at Completion over that at Onset, and the difference in months can be read roughly by referring the arrow to the age scale along the base of each figure, or more precisely by referring to the original data in the appropriate tables.

In view of these shortcomings in both the amount and the interpretation of survey-type findings on public opinion, and considering the criticisms which can be brought against Fromm's philosophical anthropology, such a passage as the following cannot be taken seriously.

In deciding on the enterprises to be managed by family labor, compare the amount of labor that can be supplied by the family with the labor needs of various enterprises listed in table 1.

`` We can claim on the maximum amount of the bond '', Berger said.

`` As long as Mr. Khrushchev is convinced that the balance of world power is shifting his way, no amount of either smiles or toughness, neither Camp David talks nor kitchen debates, can compel him to enter fruitful negotiations ''.

It assumes that one party has an unlimited amount of information ( usually through some kind of expertise ) and can act as the ‘ information services provider ’ ( pg 268 ) while the other party acts as the ‘ information services consumer ’ ( Bordewijk and Kaam, 1986: 268 )

After a fixed amount of time, the copyright expires on intellectual work and it enters the public domain, where it can be used without limit.

Area can be understood as the amount of material with a given thickness that would be necessary to fashion a model of the shape, or the amount of paint necessary to cover the surface with a single coat.

Due to the fragility of the Antarctic environment, only a limited amount of transport movements can take place and sustainable transportation technologies have to be used to reduce the ecological footprint.

However, some systems have a maximum amount of energy that they can hold, and as they approach that maximum energy their entropy actually begins to decrease.

Since 4 % can be expressed as. 04, gives the amount of alcohol in terms of ml — which, when divided by, shows the number of units.

Chemical rockets store a large amount of energy in an easily released form, and can be very dangerous.

Depression can be treated by increasing the amount of available serotonin, a chemical used in the brain to transmit signals between neurons.

If the amount in the sample is below an instrument's range of measurement, the method of addition can be used.

In addition, they can develop considerable amount of shame over their inadequacy to liberate their parents from alcoholism.

The amount of alcohol that can be biologically processed and its effects differ between sexes.

Earth sheltering and windbreaks can also reduce the absolute amount of heat needed by a building.

Formally, arbitrage transactions have negative skew – prices can get a small amount closer ( but often no closer than 0 ), while they can get very far apart.

to induce specific growth patterns in that one can easily prepare a solution containing the desired amount of hormone, add it to the known volume of GM, and autoclave to both sterilize and evaporate off any solvent that may have been used to dissolve the often-polar hormones.

This chart shows that not all fish, shellfish, or the insects that they eat can tolerate the same amount of acid ; for example, frogs can tolerate water that is more acidic ( i. e., has a lower pH ) than trout.

The folds increase the surface area of the cortex and therefore increase the amount of gray matter and the amount of information that can be processed.

In the final analysis, then, the user becomes either a bull or a bear in a given instance, notwithstanding any amount of forethought and calculation, however elaborate.

The division said it would be impossible to work on the line until then because of the large amount of acid sewage from jewelry plants in the area flowing through the line, heavy vehicle traffic on Eddy Street and tide conditions.

The amount of analyte present is then determined relative to the internal standard as a calibrant.

These were due to an enormous amount of exceedingly fine dust blown to a great height by the volcano's explosion, and then globally diffused by the high atmospheric currents.

Often, these materials are then converted into finished products, and a significant amount of value is added.

The line is intended to carry the increasing amount of goods that enter Europe, at the Croatian port of Rijeka and are then transported to destinations across central and eastern Europe.

By contrast, if M ( t ) denotes the amount of money in a bank account at time t, then the function jumps whenever money is deposited or withdrawn, so the function M ( t ) is discontinuous.

The resulting hard substance, called ' clinker ', is then ground with a small amount of gypsum into a powder to make ' Ordinary Portland Cement ', the most commonly used type of cement ( often referred to as OPC ).

It measures the amount of " stuff " flowing through a surface per unit time in a " stuff fluid " of density moving with local velocity F. Its exterior derivative is then given by

Since then, a large amount of subfossil material has been collected from Mauritius, mostly from the Mare aux Songes swamp.

When the amount of muscle is increased, then more calories can be ingested without gaining weight.

If the amount to be paid at time 0 ( now ) for all the future cash flows is known, then that amount can be substituted for DPV and the equation can be solved for i, that is the internal rate of return.

The individual or institution has access to the asset for a specified amount of time, and can then sell it in the future at a specified price according to the futures contract.

: If a planet crosses ( or transits ) in front of its parent star's disk, then the observed brightness of the star drops by a small amount.

However practical devices have a limit to the peak current they can pass, and the pulse must therefore be widened, to around 120 degrees, to obtain a reasonable amount of power, and the efficiency is then 60-70 %.

The effect of gravity on light was then explored by Johann Georg von Soldner ( 1801 ), who calculated the amount of deflection of a light ray by the sun, arriving at the Newtonian answer which is half the value predicted by general relativity.

If a significant amount of greens work is required in a film, then the Greens may be an identifiable sub-department, with its own team-often of a size numbering double figures-and hierarchy ( eg.

If the amount of orthopyroxene is substantially greater than the amount of clinopyroxene, the rock is then a norite.

However, if the vehicle travels for a certain amount of time at a speed x and then the same amount of time at a speed y, then its average speed is the arithmetic mean of x and y, which in the above example is 50 kilometres per hour.

The same principle applies to more than two segments: given a series of sub-trips at different speeds, if each sub-trip covers the same distance, then the average speed is the harmonic mean of all the sub-trip speeds, and if each sub-trip takes the same amount of time, then the average speed is the arithmetic mean of all the sub-trip speeds.