Help


[permalink] [id link]
+
Page "SDS-PAGE" ¶ 7
from Wikipedia
Edit
Promote Demote Fragment Fix

Some Related Sentences

gels and typically
Gelatin gels exist over only a small temperature range, the upper limit being the melting point of the gel, which depends on gelatin grade and concentration ( but is typically less than 35 ° C ) and the lower limit the freezing point at which ice crystallizes.
A tungsten lamp's color is typically controlled by inserting one or more gels into its optical path.
People with normal skin can choose from a wide variety of moisturizers, but light lotions or gels are typically all that is required.
Eye creams are typically very light lotions or gels, and are usually very gentle ; some may contain ingredients such as caffiene or vitamin K to reduce puffiness and dark circles under the eyes.
In the theater, gels are typically available in single 20 × 24-inch ( 500 × 600 mm ) sheets, which are then cut down to the appropriate size before use.
Ballistic gels made from natural gelatin are typically yellow-brown in color, and are not re-usable.
In persons with naturally curly or straight hair, the hairstyle is typically created with the help of creams, gels or other solidifying liquids to hold the hair in place.
A category typically referred to as " ethnic " gels are designed and manufactured specifically for sculpting the hair texture common to African Americans.

gels and consist
Non-oral herbal uses consist of creams, baths, oils, ointments, gels, distilled waters, washes, poultices, compresses, snuffs, steams, inhaled smoke and aromatics volatile oils.

gels and acrylamide
The gels are slightly more opaque than acrylamide or agarose.
Determining how much of the various solutions to mix together to make gels of particular acrylamide concentration is possible.
An additional benefit of using buffers with lower pH values is that the acrylamide gel is more stable at lower pH values, so the gels can be stored for long periods of time before use.
The fragments in the four reactions are electrophoresed side by side in denaturing acrylamide gels for size separation.

gels and SDS
Proteins may therefore be separated roughly according to size ( and thus molecular weight ); however certain glycoproteins behave anomalously on SDS gels.
Silver stained SDS Polyacrylamide gels
By far the most common type of gel electrophoresis employs polyacrylamide gels and buffers loaded with sodium dodecyl sulfate ( SDS ).

gels and buffer
" Most agarose gels are made with between 0. 7 % ( good separation or resolution of large 5 – 10kb DNA fragments ) and 2 % ( good resolution for small 0. 2 – 1kb fragments ) agarose dissolved in electrophoresis buffer.

gels and with
Depending on how much the paint is diluted ( with water ) or modified with acrylic gels, media, or pastes, the finished acrylic painting can resemble a watercolor or an oil painting, or have its own unique characteristics not attainable with other media.
However, colloidal suspensions of higher-volume fraction form colloidal gels with viscoelastic properties.
Lights with color gels were set up behind them for illumination.
Instead high percentage agarose gels should be run with a pulsed field electrophoresis ( PFE ), or field inversion electrophoresis.
The gels can be stained with ethidium bromide ( EtBr ) and viewed under UV light to observe the quality and quantity of RNA before blotting.
This technique was designed to capture the effect of the live performances of Loie Fuller, beginning in 1891, in which stage lights with colored gels turned her white flowing dresses and sleeves into artistic movement.
* The cells of cooked potatoes and legumes are gels filled with gelatinized starch granules.
Prosthetic devices commonly use silicone, urethane or elastomeric gels fit directed to the residual limb and hold the the prosthetic device with or without pin locks.
While Lathe and Ruthven used starch gels as the matrix, Jerker Porath and Per Flodin later introduced dextran gels ; other gels with size fractionation properties include agarose and polyacrylamide.
Eye creams or gels should be applied over the entire eye area with the ring finger in a patting motion.
* Kappa forms strong, rigid gels in the presence of potassium ions ; it reacts with dairy proteins.
Cockroach infestations have been treated with insecticidal sprays, dusts, gels, sand baits, etc.
Water-based gels, such as wire-pulling lubricant gel, are a good compromise ; they offer the efficacy of water with less mess, as they do not run or drip.
In addition to hand washing with soap and water, the use of alcohol gels is another form of killing some kinds of pathogens and healthful bacteria, but their effectiveness is disputed, and may lead to antibiotica-resistant bacterial strains.
Examples include ultra-strong fibers, fabrics, adhesives and composites for vehicles, bio-compatible materials for implants and prosthetics, gels for medical applications, pharmaceuticals, and films with special dielectric, optical or spectroscopic properties for opto-electronic devices.
To produce these effects, the animators used different techniques, such as drybrush, airbrush, charcoal, grease pencil, backlit animation or, during shooting, the cameraman used multiple exposures with diffusing screens, filters or gels.

gels and pH
IEF involves adding an ampholyte solution into immobilized pH gradient ( IPG ) gels.

gels and .
Other types of amorphous solids include gels, thin films, and nanostructured materials.
Viscoelastic colloidal gels, such as bentonite and toothpaste, flow like liquids under shear, but maintain their shape when shear is removed.
Common types of mufflers include muffling rings, gels and tape, and placing one's wallet near the edge of the head.
This excludes liquids or gels which are spontaneously flammable when exposed to water or the atmosphere, which belong in group H. Examples include liquid or gel filled incendiary ammunition, fuel-air explosive ( FAE ) devices, and flammable liquid fueled missiles.
Color temperature issues such as these can be compensated for by other factors such as lens filters and color gels placed in front of the lights.
Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules ; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied.
Since passing current through a gel causes heating, gels may melt during electrophoresis.
Agarose gels are easily cast and handled compared to other matrices, because the gel setting is a physical rather than chemical change.
Low percentage gels are very weak and may break when you try to lift them.
High percentage gels are often brittle and do not set evenly.
1 % gels are common for many applications.
Agarose gels do not have a uniform pore size, but are optimal for electrophoresis of proteins that are larger than 200 kDa.
Traditional DNA sequencing techniques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in length so the sequence could be read.
Most modern DNA separation methods now use agarose gels, except for particularly small DNA fragments.
For instance, gels used in SDS-PAGE ( sodium dodecyl sulfate polyacrylamide gel electrophoresis ) will unfold and denature the native structure of a protein.
Photographs can be taken of gels, often using Gel Doc.

0.140 seconds.