# A domain on the reverse transcriptase enzyme called RNAse H degrades the 5 ’ end of the RNA which removes the U5 and R region

In the third schematic, the probe and restriction enzyme are chosen to detect a region of the genome that includes a variable VNTR segment ( boxes ).

Exactly what causes this variation is not entirely understood, although factors contributing to it could include the lengths of the polyglutamine and polyglycine tracts, sensitivity to and variations in the intrauterine endocrine milieu, the effect of coregulatory proteins that are active in Sertoli cells, somatic mosaicism, expression of the 5RD2 gene in genital skin fibroblasts, reduced AR transcription and translation from factors other than mutations in the AR coding region, an unidentified coactivator protein, enzyme deficiencies such as 21-hydroxylase deficiency, or other genetic variations such as a mutant steroidogenic factor-1 ( SF-1 ) protein.

* Multiple cloning site, the region of restriction enzyme sites in a plasmid

The juxtaglomerular apparatus is a specialized region of the nephron responsible for production and secretion of the enzyme renin, involved in the renin-angiotensin system.

Unlike the SET domain, which targets the lysine tail region of the histone, Dot1 methylates a lysine residue in the globular core of the histone, and is the only enzyme known to do so.

This is usually aided by a single-stranded region of the RNA, and the loading of the enzyme is accompanied with ATP binding.

Located close to the 240s loop and the active site, the loop region encompassing residues 160-166 plays a role in both the internal architecture of the enzyme and its regulatory properties.

Field effect transistors, in which the gate region has been modified with an enzyme or antibody, can also detect very low concentrations of various analytes as the binding of the analyte to the gate region of the FET cause a change in the drain-source current.

The enzyme also contains a nucleic acid binding cleft about 60 Å in length that can encompass a region of 18 bound RNA / DNA base pairs.

Removal of this region by proteolytic cleavage results in activation of the enzyme.

These cyclins have a nine residue sequence in the N-terminal region called as the “ destruction box ”, which can be recognized by the ubiquitin ligase enzyme and thus destroys the cyclins.

The small domain consists of residues 15-47 and 326-410 and represents a flexible region that shifts the enzyme from an " open " to a " closed " conformation upon substrate binding.

The pseudosubstrate region, which is present in all three classes of PKC, is a small sequence of amino acids that mimic a substrate and bind the substrate-binding cavity in the catalytic domain, lack critical serine, threonine phosphoacceptor residues, keeping the enzyme inactive.

Two examples of these conserved signature indels are a 2 amino acid insertion in a conserved region of the enzyme phosphoribose diphosphate: decaprenyl-phosphate phosphoribosyltransferase and a 3 amino acid insertion in acetate kinase, both of which are found only in < i > Corynebacterium </ i > species.

Its polylinker region is composed of several restriction enzyme recognition sites, that have been engineered into a single cluster ( the polylinker ).

For example, AMY1C has a complete HERV sequence in its promoter region ; the associated LTR confers salivary specific expression of the digestive enzyme, amylase.

There are also high levels of aromatase, an estrogen-generating enzyme, in the hindbrain vocal motor region.

Finally, N-terminal region modulates PFK2 and FBPase2 activities, and stabilizes the dimer form of the enzyme.

It has sites for amino acid attachment and an anticodon region for codon recognition that binds to a specific sequence on the messenger RNA chain through hydrogen bonding.

# Complementary DNA then binds to the U5 ( non-coding region ) and R region ( a direct repeat found at both ends of the RNA molecule ) of the viral RNA

It also has been shown that the N-terminal region of reelin, a site distinct from the region of reelin shown to associate with VLDLR / ApoER2 binds to the alpha-3-beta-1 integrin receptor.

By using an enzyme-linked antibody that binds the Fc region of other antibodies, this same enzyme-linked antibody can be used in a variety of situations.

The degree of cooperativity is characterized by the maximum slope in the " ramping up " region, which is ~ 2. 8 for hemoglobin ; thus, at its most cooperative, hemoglobin effectively binds three ligands in concert.

Protein A, an IgG-binding protein, binds to the Fc region of an antibody.

A ligand produced by one cell binds to a receptor in the extracellular region of another cell, inducing a conformational change in the receptor.

The transactivator gene expresses a transcription factor that binds to specific promoter region of DNA.

These two functional subunits are coupled at the tetramerization region ( labeled ); thus, tetrameric LacI binds two operator sequences.

The binding changes the structural conformity of the repressor such that it binds to the operator region for the operon that synthesizes tryptophan, preventing their expression and thus suspending production.

Rho factor binds to the transcription terminator pause site, an exposed region of single stranded RNA ( a stretch of 72 nucleotides ) after the open reading frame at GC-rich sequences that lack obvious secondary structure.

Rho binds to RNA and then uses its ATPase activity to provide the energy to translocate along the RNA until it reaches the RNA-DNA helical region, where it unwinds the hybrid duplex structure.

Each of the two tips of Fab region is the paratope, which binds a matching molecular sequence and its 3D shape ( conformation ), altogether called epitope, within the target antigen.

The IgG's Fc region also enables opsonization by a phagocyte, a process by which the Fc receptor on the phagocyte — such as neutrophils in blood and macrophages in tissues — binds the antibody molecule's FC stalk, and the phagocyte exhibits enhanced uptake of the antigen, attached to the antibody molecule's Fab region.

When the paratope of Ig class gamma ( IgG ) binds its matching epitope, IgG's Fc region conformationally shifts and can host a complement protein, initiating the complement cascade that terminates by punching a hole in a cell membrane.

After a hormone binds to the corresponding receptor, the newly formed receptor-ligand complex translocates itself into the cell nucleus, where it binds to glucocorticoid response elements ( GRE ) in the promoter region of the target genes resulting in the regulation of gene expression.

After a hormone binds to the corresponding receptor, the newly formed receptor-ligand complex translocates itself into the cell nucleus, where it binds to many hormone response elements ( HREs ) in the promoter region of the target genes in the DNA.

Bicoid binds a specific RNA sequence in the 3 ' untranslated region, called the Bicoid 3 '- UTR regulatory element, of caudal mRNA and blocks translation.

In general, this sequence occurs in the promotor region of a gene, and, when the PPAR binds its ligand, transcription of target genes is increased or decreased, depending on the gene.

The pictures of Figs. 3 and 4 show the cracking and peeling types of removal where the coating is detached by failure in a region at, or close to, the interface between coating and substrate.

** Remote plasma-enhanced CVD ( RPECVD ) – Similar to PECVD except that the wafer substrate is not directly in the plasma discharge region.

This exposed region can then be removed or treated providing a mask for the underlying substrate.

Such changes are often induced by the binding of a substrate molecule to an enzyme's active site, or the physical region of the protein that participates in chemical catalysis.

These enzymes contain a conserved motif Asp / Asn-Pro-Pro-Tyr / Phe in their N-terminal section, this conserved region could be involved in substrate binding or in the catalytic activity.

It is normally used as the absorption region of a heterostructure diode, most typically involving InP as a substrate and as a multiplication layer.

; Edaphic Climax: When there are more than one climax communities in the region, modified by local conditions of the substrate such as soil moisture, soil nutrients, topography, slope exposure, fire, and animal activity, it is called edaphic climax.

The C-terminal is important for the substrate specificity and binding of Dot1 because the region carries a positive charge, allowing for a favorable interaction with the negatively charged backbone of DNA.

Gujarati has also been suggested as a possible substrate, but this is doubtful, since the Gujarati people moved into the region only after the Portuguese arrived.

The sputtered species from the substrate and the particles emitted from the target form a collision region, which serves as a source for condensation of particles.

The PDMS stamp is indicated by the hatched region, and the substrate is indicated by the shaded region.

* Single-event induced burnout ( SEB ) may occur in power MOSFETs when the substrate right under the source region gets forward-biased and the drain-source voltage is higher than the breakdown voltage of the parasitic structures.

Upon binding to Apo-CII and lipid in the lipoprotein, the C-terminal domain presents the lipid substrate to the lid region.

Linguist Peter Schrijver speculates on the reminiscent lexical and typological features of the region, from an unknown substrate whose linguistic influences may have influenced the historical development of the ( Romance and Germanic ) languages of the region.

Among the potential problems of the technique are the difficulty of correctly identifying the species, delay between exposure and colonization, varying growth rates from region to region as well as the fact that growth rates are not always constant over time, dependence of the rate of growth upon substrate texture and composition, climate, and determining which lichen is the largest.

Notable candidates for possible substrate culture ( s ) are the Ertebølle culture, Funnelbeaker culture, Pitted Ware culture and the Corded Ware culture, but also the oldest cultures of northern Europe like the Hamburgian, the first distinct culture identified of this region.

This structure suggests that there is no depletion region to separate the device from the substrate.

If the gate material is polysilicon of opposite type to the bulk semiconductor, then a spontaneous depletion region forms if the gate is electrically shorted to the substrate, in much the same manner as described for the pn-junction above.

This furrow, which may be broken, set at an angle, and of varying width and depth, is thought to be the result of the tail region contacting the substrate.