These cells were thawed at 37-degrees-C for 30 min and were deglycerolized by alternately centrifuging and mixing with descending concentrations of glycerol solutions ( 20, 18, 10, 8, 4 and 2% ).

These included Oregon State Fair, for which he had been booked on and off, for 30 years.

These conditions apparently had little effect in the deep tropics, where lush coal swamps flourished within 30 degrees of the northernmost glaciers.

These plants compose a shrub cover up to 30 cm in height and are intermixed with Eclipta, Phyllanthus, and Solanum, as well as a taller plant, Brassica juncea.

These results show that the composition of the salt, as anhydrous chlorides on a weight percentage basis, was calcium chloride ( CaCl < sub > 2 </ sub >) 14. 4 %, potassium chloride ( KCl ) 4. 4 %, magnesium chloride ( MgCl < sub > 2 </ sub >) 50. 8 % and sodium chloride ( common salt, NaCl ) 30. 4 %.

These studies report a 30 % reduction in weekly seizures.

These plant communities cover approximately 9. 4 percent of the Earth's surface ( or 30 percent of total land area ), though they once covered much more ( about 50 percent of total land area ), in many different regions and function as habitats for organisms, hydrologic flow modulators, and soil conservers, constituting one of the most important aspects of the biosphere.

These licensing fees account for nearly 30 % of domestic budgetary revenue.

These three saints, in addition to having their own individual commemorations throughout the year, are commemorated together on 30 January, a feast known as the Synaxis of the Three Hierarchs.

These had four turrets mounting two 30. 5 cm guns arranged in two turrets either end, with the inner turret able to fire over the outer.

These connect with Mainline services which operate at a frequency of between 10 and 30 minutes.

These Berber militias were able to resist the French and Spanish armies for over 30 years, from 1907 to 1933.

These clouds would have a typical density of 30 particles per cubic centimeter.

These executions took place in over 30 towns and villages in the first month of German occupation alone.

These officials must be from at least 30 départements or overseas collectivities, and no more than 10 % of them should be from the same département or collectivity.

These emperors would carefully add new legions, as circumstances required or permitted, until the strength of the standing army stood at around 30 legions ( hence the wry remark of the philosopher Favorinus that It is ill arguing with the master of 30 legions ).

These voluntary prayers are held in the mosques every night of the month, during which a whole section of the Quran ( Juz ', which is 1 / 30 of the Quran ) is recited.

These fourteen orders contain 31 virus families ( including 17 families of plant viruses ) and 48 genera ( including 30 genera of plant viruses ).

These letters, of which 17, 500 have been collected thus far and are believed to number of 30, 000, ranged from routine correspondence regarding the affairs of Bahá ' ís around the world to lengthy letters to the Bahá ' ís of the world addressing specific themes.

These include the Attorney General, five members elected from the Zanzibar House of Representatives to participate in the Parliament, the special women's seats which are made up of 30 % of the seats that a given party has in the House, 181 constituent seats of members of Parliament from the mainland, and 50 seats from Zanzibar.

These were followed by riots over rising food prices in 1991, in which at least 30 people were killed.

These elections were known as the " Flapper " elections due to the fact that it was the first British election in which women under 30 could vote.

These areas would have been strictly " free territory " by the Missouri Compromise, which allowed slavery in the State of Missouri but disallowed it everywhere north of the latitude 36 degrees, 30 minutes, which forms most of the southern boundary of Missouri.

These pitted surfaces further reduce the flow of water through the xylem by as much as 30 %.

These caches typically use very short caching times — on the order of one minute.

These subdivisions are often segregated by minute differences in home value, creating entire communities where family incomes and demographics are almost completely homogeneous ..

These clocks differ from the ‘ impulse ’ type, which are electrically advanced each minute by the master clock.

These are lined with numerous minute ciliated funnels that open directly into the body cavity, and are presumed to be excretory organs.

" These weapons attract then repel the target ( entire ship or a segment of the ship's hull ) at 80 gs, several times a minute.

These included Tango Suite for the virtuoso guitar duo Sergio and Odair Assad ; Histoire du Tango, where a flutist and guitarist tell the history of tango in four chunks of music styled at thirty-year intervals ; and La Camorra, a suite in three ten minute movements, inspired by the Neapolitan crime family and exploring symphonic concepts of large-scale form, thematic development, contrasts of texture and massive accumulations of ensemble sound.

These can be reconstructed from the minute variations of size of the paper strips and the microscopic marks on their edges, and by comparison with the strips produced by known shredders, the individual shredder that was used to destroy a given document may be determined.

These symptoms are transitory, usually lasting less than one minute.

These are rooms where someone doing a 10-15 minute set ( hosting / MCing ) can be asked, after they've been going up long enough, to do a 20 minute set ( featuring ) and so on.

These time differences were minute, but the improvement in electronics made it possible to measure them accurately and identify the presence of metal at a reasonable distance.

These are two minute segments before the theme song and actual show.

These rules prescribe the use of gloves, the ten second count, the three minute round and the one minute's rest between rounds.

These predators include green lacewings, ladybirds, minute pirate bugs, big-eyed bugs, and damsel bugs .</ br >

These are quick, three minute, automated tests that most hospital laboratories can easily perform.

These releases featured episodes edited into 70-80 minute movies based on the particular story arc.

These two riders worked together and soon built up a minute lead over the others.

These terminals use the same airside facilities but are physically separated on the cityside, requiring a 15 – 20 minute ( airside ) drive between them.

These segments, though carefully disclaimed after concerns were brought up about the original program model of Daytime, are designed to give a business a detailed presentation of their service that might not be possible in a traditional thirty-second or one minute ad, or a declining form of advertising where a special sale for a certain time period with a sales pitch is offered within the false setting of a phone bank or telethon.

These seemingly minute changes in a monoclonal antibody ’ s structure can have a profound effect on preclinical stability and process optimization as well as therapeutic product potency, bioavailability, and immunogenicity.

These species are minute organisms that prefer to feed within the tightly packed apical buds of new growth.

These sounds are produced 14 to 40 times a minute.

These included Plácido Domingo flying into San Francisco — albeit three hours after curtain time-to replace the ailing Carlo Cossutta on the opening night of Otello, and the last minute substitution by Leontyne Price for Margaret Price in the role of Aida.

These writings maintain that Divine Providence means that God is directing ( or even recreating ) every minute detail of creation.