A restriction enzyme ( or restriction endonuclease ) is an enzyme that cuts DNA at specific recognition nucleotide sequences ( with Type II restriction enzymes cutting double-stranded DNA ) known as restriction sites.

* Type I enzymes () cleave at sites remote from recognition site ; require both ATP and S-adenosyl-L-methionine to function ; multifunctional protein with both restriction and methylase () activities.

* Type II enzymes () cleave within or at short specific distances from recognition site ; most require magnesium ; single function ( restriction ) enzymes independent of methylase.

Type I restriction enzymes were the first to be identified and were first identified in two different strains ( K-12 and B ) of E. coli.

Type I restriction enzymes possess three subunits called HsdR, HsdM, and HsdS ; HsdR is required for restriction ; HsdM is necessary for adding methyl groups to host DNA ( methyltransferase activity ) and HsdS is important for specificity of the recognition ( DNA-binding ) site in addition to both restriction ( DNA cleavage ) and modification ( DNA methyltransferase ) activity.

Type IIB restriction enzymes ( e. g. BcgI and BplI ) are multimers, containing more than one subunit.

Type IIG restriction endonucleases ( Eco57I ) do have a single subunit, like classical Type II restriction enzymes, but require the cofactor AdoMet to be active.

Type IIM restriction endonucleases, such as DpnI, are able to recognize and cut methylated DNA.

Type IIS restriction endonucleases ( e. g. FokI ) cleave DNA at a defined distance from their non-palindromic asymmetric recognition sites.

Similarly, Type IIT restriction enzymes ( e. g., Bpu10I and BslI ) are composed of two different subunits.

Type III restriction enzymes ( e. g. EcoP15 ) recognize two separate non-palindromic sequences that are inversely oriented.

Type I systems are the most complex, consisting of three polypeptides: R ( restriction ), M ( modification ), and S ( specificity ).

Type safety is closely linked to memory safety, a restriction on the ability to copy arbitrary bit patterns from one memory location to another.

Restriction endonucleases ( restriction enzymes ) and are divided into three categories, Type I, Type II, and Type III, according to their mechanism of action.

Type of data structure selected for a certain task typically also takes into consideration the type of storage it resides in ( e. g., speed of access, minimal size of storage chunk accessed, etc .).

Type of data structure selected for a certain task typically also takes into consideration the type of storage it resides in ( e. g., speed of access, minimal size of storage chunk accessed, etc .).

Image: Insigne de béret du 2e RE Type 3. jpg |< center > 2 < sup > e </ sup > REI < center >

Type 2 products may not be used for classified information, but contain classified NSA algorithms ( e. g. CORDOBA ) that distinguish them from products containing unclassified algorithms like DES.

< li > Type I coactivators ( i. e., coregulators ) are thought to influence AR transcriptional activity by facilitating DNA occupancy, chromatin remodeling, or the recruitment of general transcription factors associated with RNA polymerase II holocomplex.

* Type B, those evaluated by other means, e. g., by assigning a probability distribution

A Type 0 civilization extracts its energy, information, raw-materials from crude organic-based sources ( i. e. Food / Wood / Fossil Fuel ).

The population of Ninove has steadily grown and the city has become an attractive centre for commercial, recreational and economical activities in the area, and plays host to a popular annual Volkswagen show every March, specifically for air-cooled vehicles, i. e. Beetles, Camper-vans, Type 3's, 4's etc.

There are two types: Type Cheylan: captures on the rebirth square are illegal ( i. e. a white rook can't capture on a1 ).

An alternative ( but nevertheless related ) statistical hypothesis testing framework is the Neyman – Pearson frequentist school which requires both a null and an alternative hypothesis to be defined and investigates the repeat sampling properties of the procedure, i. e. the probability that a decision to reject the null hypothesis will be made when it is in fact true and should not have been rejected ( this is called a " false positive " or Type I error ) and the probability that a decision will be made to accept the null hypothesis when it is in fact false ( Type II error ).

Since the end of the Second World War, Royal Navy ship classes have also been known by their type number ( e. g. Type 42 destroyer.

Because the IOS needs to know the cleartext password for certain uses, ( e. g., CHAP authentication ) passwords entered into the CLI by default are weakly encrypted as ' Type 7 ' ciphertext, such as "< tt > Router ( config )# username jdoe password 7 0832585B1910010713181F </ tt >".

They are now given a fractional classification depending upon the relative strengths of the narrow and broad components ( e. g. Type 1. 5 or Type 1. 9 ).

The power of a statistical test is the probability that the test will reject the null hypothesis when the null hypothesis is false ( i. e. the probability of not committing a Type II error, or making a false negative decision ).

This increases the chance of rejecting the null hypothesis ( i. e. obtaining a statistically significant result ) when the null hypothesis is false, that is, reduces the risk of a Type II error.

But it also increases the risk of obtaining a statistically significant result ( i. e. rejecting the null hypothesis ) when the null hypothesis is true ; that is, it increases the risk of a Type I error.

However, when any of these tests are conducted to test the underlying assumption of homoscedasticity ( i. e. homogeneity of variance ), as a preliminary step to testing for mean effects, there is an increase in the experiment-wise Type I error rate .< ref > Sawilowsky, S. ( 2002 ).

In 1992 Huet and Coquand introduced the calculus of constructions, a type theory with an impredicative universe, thus combining Type Theory with Girard's System F. This extension is not universally accepted by Intuitionists since it allows impredicative, i. e. circular, constructions, which are often identified with classical reasoning.

For padding the seats and bunks, I used Ensolite, Type Aj.

More unitized lumber is being shipped on flat cars, and NLRDA studies show that flat cars loaded with the new Type 6-B floating-load method can be unloaded for as little as $.054/mbf.

Post hoc tests such as Tukey's range test most commonly compare every group mean with every other group mean and typically incorporate some method of controlling for Type I errors.

* ASL Helper Type an English word, links to vocabulary sites.

His contemporaries called it Aldine Type ; today we call it italics.

* 2010 – Peter Steele, American singer, bassist, and composer ( Type O Negative, Carnivore, and Fallout ) ( b. 1962 )

An individual with " Type A " blood may be an AO heterozygote, an AA homozygote, or an A ' A heterozygote with two different ' A ' alleles.

AEP generates packets to be sent to the network node and is identified in the Type field of a packet as an AEP packet.

About 26 were sold to Siam and saw action as Infantry support guns and AA guns during the Franco-Thai war ( 1940-1941 ) along with 30 Vickers Mk. E Type B 6-ton tanks.

Modern weapons include the Russian ZSU-23-4 Shilka and Tunguska-M1, South Korean K30 Biho and K263A1 radar-guided Vulcan, Chinese Type 95 SPAAA, Swedish CV9040 AAV, Polish PZA Loara, American M6 Bradley Linebacker and M1097 Humvee Avenger, Yugoslavian BOV-3, Canadian ADATS, aging German Gepard, Japanese Type 87 SPAAG and similar versions with the British Marksman turret ( which was also adapted for a number of other users ), Italian SIDAM 25 and Otomatic, and versions of the French AMX-13.

EPD and PPD work with AAL5 connections as they use the end of packet marker: the ATM User-to-ATM User ( AUU ) Indication bit in the Payload Type field of the header, which is set in the last cell of a SAR-SDU.

* Bloomberg News, FTC Says Alcohol Type Not Aimed at Minors, Los Angeles Times, June 5, 2002.

Even though alcoholism can increase the risk of liver cancer, studies have shown that a moderate consumption of alcohol ( 1 serving / day for women and 2 servings / day for men ) does not affect diabetes Type II greatly.

In addition, the navy contracted in late 1994 to acquire four Type 22 British Royal Navy frigates and three River-class minesweepers for delivery in the 1995-97 period.

Osteoid is primarily composed of Type I collagen.

The organic part of matrix is mainly composed of Type I collagen.

The core ideas of the Big Bang — the expansion, the early hot state, the formation of helium, and the formation of galaxies — are derived from many observations that are independent from any cosmological model ; these include the abundance of light elements, the cosmic microwave background, large scale structure, and the Hubble diagram for Type Ia supernovae.

Based on measurements of the expansion using Type Ia supernovae, measurements of temperature fluctuations in the cosmic microwave background, and measurements of the correlation function of galaxies, the Universe has a calculated age of 13. 75 ± 0. 11 billion years.

Independent lines of evidence from Type Ia supernovae and the CMB imply that the Universe today is dominated by a mysterious form of energy known as dark energy, which apparently permeates all of space.

* Movable Type ( until version 4. 0 ) – A proprietary weblog publishing system developed by California-based Six Apart

* Type collections: Often a collection consists of an examples of major design variants for a period of time in one country or region.