With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With a recreational abalone licence, there is a bag limit of 10 per day, and a total possession limit of 20.

With these elements, hydride atomization enhances detection limits by a factor of 10 to 100 compared to alternative methods.

With a reserve ratio of 10 %, the bank can create 400 million USD in additional loans ( there is a time lag, and the bank has to expect to recover the loaned money back into its books ).

With United Nations Security Council Resolution 1244, the bombing campaign ended on June 10, 1999.

With around 10, 000 living species, they are the most speciose class of tetrapod vertebrates.

With the Mirage III withdrawn, the air defence of Brasília and region is done temporarily by a mix of F-5s from 1st GAv and 1st / 14th GAv until 10 Mirage 2000Cs and two Mirage 2000B bought from French Air Force surplus stocks arrive at Anápolis.

With 20, 000 men ( including a rate of feminization of nearly 10 %) the Army remains the most important component in terms of numbers.

With over 10 million foreign tourists annually, tourism generates revenue in excess of € 7 billion.

" With total world emissions from fossil fuels growing rapidly, the proportion of fossil-fuel emissions absorbed by the oceans since 2000 may have declined by as much as 10 %, indicating that over time the ocean will become " a less efficient sink of manmade carbon.

* 1978 – With the crew investigating a problem with the landing gear, United Airlines Flight 173 runs out of fuel and crashes in Portland, Oregon, killing 10.

With one third of the game ( 9 levels ) distributed as shareware, Doom was played by an estimated 10 million people within two years of its release, popularizing the mode of gameplay and spawning a gaming subculture ; as a sign of its effect on the industry, games from the mid-1990s boom of first-person shooters are often known simply as " Doom clones ".

With 2, 000 congregations and a membership of some 1. 8 million ( or 10. 8 % of the Dutch population, 2009 ), it is the second largest church in the Netherlands after the Roman Catholic Church.

With ADR, actors can average 10 – 12 lines per hour, while rythmo band can facilitate the reading of 35-50 lines per hour.

With such business implications in mind, David Liddle ( General Manager, Xerox Office Systems ) and Metcalfe ( 3Com ) strongly supported a proposal of Fritz Röscheisen ( Siemens Private Networks ) for an alliance in the emerging office communication market, including Siemens ' support for the international standardization of Ethernet ( April 10, 1981 ).

With a $ 10 million dollar investment, Paramount built their own chain of first-run movie theaters after a secret plan to merge with First National failed.

Line No. 2 PC ( inner ring 2 ), serves the same areas as the PC one in reverse. With a bus every hour from Monday to Saturday. Amplitude ( 1 bus ): 6 h beginning 10, 20 end 10 h.

With McMahon unexpectedly eliminated from the contest, Senator John Gorton was elected Liberal leader on 9 January 1968, and was sworn in as Prime Minister on 10 January, replacing McEwen.

With an annual drift of 10 or 11 days, the seasonal relation repeats about every 33 Islamic years.

With average growth rates of 10 % in the 1960s, 5 % in the 1970s, and 4 % in the 1980s, Japan was able to establish and maintain itself as the world's second largest economy from 1968 until 2010, when it was supplanted by the People's Republic of China.

" With the help of record producers Herb Alpert and Lou Adler, Jan and Dean scored a No. 10 hit with " Baby Talk " ( 1959 ), their first song to contain a few of the soon to be famous elements that became associated with surf ( close vocal harmonies, selective use of major and minor chords, falsetto doo-wop singing ) and then scored a series of hits over the next couple of years.

With numbers, Finnish does not use plurals but partitive singular forms: " 10 markkaa " and " 10 penniä " ( the nominative is penni ).

With the support of Prince Louis the French Heir and of King Alexander II of the Scots, they entered London in force on 10 June 1215, with the city showing its sympathy with their cause by opening its gates to them.

With Brown at the helm, the team won all four of the AAFC's championships from 1946 until its dissolution in 1949, amassing a record of 52 wins, four losses and three ties.

With wins of 13 – 0 and 4 – 2, the Cubs needed to win only one game of the next three in San Diego to make to the World Series.

With wins at Atlanta, Bristol, Nashville, Martinsville, and Charlotte, Earnhardt won his first Winston Cup championship.

With perfect play, either the first player wins or the result is a draw.

With two wins each, Brabham, Moss and Ferrari's Tony Brooks were all capable of winning the championship at the final event of the season, the United States Grand Prix at Sebring.

With over 100 wins including playoff victories.

With a roll of the dice, Death wins the lives of the crew members and Life-in-Death the life of the Mariner, a prize she considers more valuable.

With 31 Grammy Awards, Sir Georg Solti is the artist with the most Grammy wins.

With NHL superstars Marian Hossa and Ilya Kovalchuk and a healthy goaltender, Kari Lehtonen, the Thrashers clinched their first playoff berth, winning the Southeast Division title with 43 wins and claiming the # 3 seed and home-ice advantage in the first round of the playoffs.

With a set starting rotation of Battie, Howard, Türkoğlu, DeShawn Stevenson, and Nelson, the Magic mounted a surprising run at the eighth playoff spot in the Eastern Conference, including an 8-game winning streak and twelve consecutive home wins.

With the condensed 2011-12 NBA season due to the lockout, the Mavericks were unable to win 50 games, thus ending their 11 consecutive season with 50 or more wins.

The 1962 episode " A Date With Jet Screamer ", in which daughter Judy Jetson wins a date with a rock star, provided the song " Eep Opp Ork Ah-Ah ( Means I Love You )" written by Hoyt Curtin, William Hanna and Joseph Barbera, sung by Howard Morris.

With a 9 – 5 win over the Milwaukee Brewers on July 29, 1986, Anderson became the first to achieve 600 career wins as a manager in both the American and National Leagues.

With outstanding beauty and intelligence she wins the hearts of Seregay and Lee Geun in a single spell.

With six straight wins, Molde stayed in the top two positions of the league throughout the season, but eventually finished second 15 points behind Rosenborg.

With 16 wins, the team did not suffer a single defeat during the championship, a deed yet unmatched by any other club in Brazil.

With the exception of a couple of Campeonato Mineiro wins, the club won no other championships in the 1980s, and had its worst performances in the Campeonato Brasileiro, 33rd in 1984 and 29th in 1985.

With that knockout win, a streak of 22 knockout wins in a row had been set off.

With advice from Emerson Fittipaldi, Piquet went to Europe to further success by taking the record number of wins in Formula Three in 1978, defeating Jackie Stewart's all-time record.

With the gang out of the way, Speed finishes and wins the race ... however, he does not win the prize money since officials had found out that the race was sabotaged by the corporate gang.

With Arizona, he went 22 – 6 with a 2. 98 ERA in 2001, leading the majors in wins and innings pitched.

With 33 wins and 45 losses, the Stars improved from their first season but again finished fifth in the Western Division and did not make the playoffs.

With his team cruising to the easiest of wins, Hughes decided to save some money by leaving Dyer on the bench all day.

With the mercurial John Horton and the incisive Mike Beese, the side continued to develop Bath's reputation in the early Seventies with some spectacular wins over the cream of Welsh rugby union in its heyday.