With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With a population of about 66, 000, Aalen is the 15 most-populated settlement in Baden-Württemberg.

With the Lombard invasion of Italy it was annexed to the Duchy of Benevento, until it was finally destroyed by the Byzantines of Constans II in 663, reducing to a small hamlet known as Quintodecimo ( referring to its distance of 15 miles from Benevento ).

With the conversion of Sergius Paulus, Paul begins to gain prominence over Barnabas from the point where the name " Paul ," his Roman name, is substituted for " Saul " ( 13: 9 ); instead of " Barnabas and Saul " as heretofore ( 11: 30 ; 12: 25 ; 13: 2, 7 ) we now read " Paul and Barnabas " ( 13: 43, 46, 50 ; 14: 20 ; 15: 2, 22, 35 ); only in 14: 14 and 15: 12, 25 does Barnabas again occupy the first place, in the first passage with recollection of 14: 12, in the last two, because Barnabas stood in closer relation to the Jerusalem church than Paul.

With the acquisition of Montagu House the first exhibition galleries and reading room for scholars opened on 15 January 1759.

With this deed dating back to August 15, 805 A. D., the Feast of the Assumption of the Blessed Virgin Mary, she donated her entire property in Dachau, including 5 so called Colonenhöfe and some serfs and bondsman, to devolve to the Bishop of the Diocese of Freising after her death.

Line No. 2 dessert habitat areas such as: RESIDENCE UNIVERSITAIRE, STANILAS, PASTEUR, RESIDENCE DE BADUEL, and major traffic generators in the Greater Cayenne ( downtown and schools ). With a bus every 35 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 h beginning 15, 01 end 20 h.

* With unpaved runways: 15

He took office as the Prime Minister of India on 15 August, and delivered his inaugural address titled " A Tryst With Destiny "

With sacrifice and priesthood established, chapters 11 – 15 instruct the lay people on purity ( or cleanliness ).

With the growth of industrialism and the introduction of machinery, longer hours became far more common, with 14 – 15 hours being the norm, and 16 not at all uncommon.

* Sanger, David E., " War Figures Honored With Medal of Freedom ", The New York Times, December 15, 2004.

With liquid oxygen costing $ 0. 15 per kilogram and liquid hydrogen $ 2. 20 per kilogram, the Space Shuttle has a liquid propellant expense of approximately $ 1. 4 million for each launch that costs $ 450 million from other expenses ( with 40 % of the mass of propellants used by it being liquids in the external fuel tank, 60 % solids in the SRBs ).

With the conscript based system during mid 1995, Sweden consisted of 15 maneuver brigades and, in addition, 100 battalions of various sorts ( artillery, engineers, rangers, air defense, amphibious, security, surveillance etc.

With the arrival of Governor Cesare Maria De Vecchi on 15 December 1923, things began to change for that part of Somaliland.

With eleven free agents excluding Jason Schmidt who signed with the Dodgers for roughly $ 15 million a year, a new manager on board with Bruce Bochy coming from division rival San Diego, and the loss of veteran catcher Mike Matheny due to complications resulting from concussions sustained during his career, the Giants ' prospects for the 2007 season were less than favorable going into the winter off-season.

With just 15 seconds left in the half, Ray Wersching's ensuing squib kick was muffed by Bengals running back Archie Griffin, and the 49ers recovered the ball on the Bengals 4-yard line.

With the game almost completely out of reach, the Bills managed to respond with a 15 play, 79-yard drive to cut the score to 37 – 17 on a 2-yard touchdown pass from Kelly to Metzelaars.

With the aid of linebacker Levon Kirkland's 8-yard sack on Aikman, the Cowboys were forced to punt on their next drive and Pittsburgh regained possession of the ball at their own 32-yard line with 4: 15 remaining.

With 16 seconds left in the game, Bills kicker Steve Christie made what seemed to be the game-winning 41-yard field goal to give his team the lead, 16 – 15.

With respect to the issues, Dole promised a 15 % across-the-board reduction in income tax rates and made former Congressman and supply side advocate Jack Kemp his running mate.

With E =-9. 15 for the S-methyldibenzothiophenium ion, typical nucleophile values N ( s ) are 15. 63 ( 0. 64 ) for piperidine, 10. 49 ( 0. 68 ) for methoxide, and 5. 20 ( 0. 89 ) for water.

With a capacity of over 72, 000, it is the second largest of its kind in Italy, with only San Siro able to seat more.

With capacity to produce up to 5, 000 engines a year by 100 specially trained personnel, like traditional Aston Martin engine production from Newport Pagnell, assembly of each unit is entrusted to a single technician from a pool of 30, with V8 and V12 variants assembled in under 20 hours.

With around 10, 000 living species, they are the most speciose class of tetrapod vertebrates.

With 830, 000 inhabitants in the tri-national urban agglomeration as of 2004, Basel is Switzerland's second-largest urban area.

With 20, 000 men ( including a rate of feminization of nearly 10 %) the Army remains the most important component in terms of numbers.

" With more than 2, 000 dead, the 44-day Costa Rican Civil War resulting from this uprising was the bloodiest event in twentieth-century Costa Rican history ", but the victorious junta drafted a constitution guaranteeing free elections with universal suffrage and the abolition of the military.

With over 55, 000 missionaries worldwide, the church currently has a membership of over 14. 4 million and is ranked by the National Council of Churches as the fourth largest Christian denomination in the United States.

With over 23, 000 modules ( containing 20, 000, 000 lines of code ) as of July 2011, the CPAN can save programmers weeks of time, and large Perl programs often make use of dozens of modules.

With over 200, 000 Chinese casualties, Chiang lost the political cream of his Whampoa-trained officers.

With berths for more than 5, 000 boats, the Chicago Park District operates the nation's largest municipal harbor system ; even larger than systems in cities such as New York City, Los Angeles, or Miami.

With four legions at his disposal, Vespasian commanded a strength of nearly 80, 000 soldiers.

With an estimated 35, 000 dolmen, Korea alone accounts for nearly 40 % of the world ’ s total.

With 2, 000 congregations and a membership of some 1. 8 million ( or 10. 8 % of the Dutch population, 2009 ), it is the second largest church in the Netherlands after the Roman Catholic Church.

With a population of approximately 3, 000, and the number of speakers in the mere hundreds, Alutiiq communities are currently in the process of revitalizing their language.

With the recovery of virtue, the human life span will gradually increase again until it reaches 80, 000 years, with people attaining sexual maturity at 500.

With a total manpower of 3, 500 active soldiers and 6, 000 reservists, it is one of the smallest militaries in the world.

With around 7, 000 personnel serving in the Ghanian military, Ghana has the tenth lowest ratio of active troops per thousand citizens in the world, standing at 0. 6.

With a large expedition that eventually included 40 000 European troops, and receiving help from white colonists and mulatto forces commanded by Alexandre Pétion, a former lieutenant of Rigaud, the French won several victories after severe fighting.

With a population of 4. 8 million in 1911, Scotland sent 690, 000 men to the war, of whom 74, 000 died in combat or from disease, and 150, 000 were seriously wounded.

With the abandonment of left-hand drive markets, Holden exported almost 100, 000 Commodores to markets such as New Zealand, Thailand, Hong Kong, Malaysia, Indonesia, Malta and Singapore.