With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With a population of about 66, 000, Aalen is the 15 most-populated settlement in Baden-Württemberg.

With the Lombard invasion of Italy it was annexed to the Duchy of Benevento, until it was finally destroyed by the Byzantines of Constans II in 663, reducing to a small hamlet known as Quintodecimo ( referring to its distance of 15 miles from Benevento ).

With the conversion of Sergius Paulus, Paul begins to gain prominence over Barnabas from the point where the name " Paul ," his Roman name, is substituted for " Saul " ( 13: 9 ); instead of " Barnabas and Saul " as heretofore ( 11: 30 ; 12: 25 ; 13: 2, 7 ) we now read " Paul and Barnabas " ( 13: 43, 46, 50 ; 14: 20 ; 15: 2, 22, 35 ); only in 14: 14 and 15: 12, 25 does Barnabas again occupy the first place, in the first passage with recollection of 14: 12, in the last two, because Barnabas stood in closer relation to the Jerusalem church than Paul.

With the acquisition of Montagu House the first exhibition galleries and reading room for scholars opened on 15 January 1759.

With this deed dating back to August 15, 805 A. D., the Feast of the Assumption of the Blessed Virgin Mary, she donated her entire property in Dachau, including 5 so called Colonenhöfe and some serfs and bondsman, to devolve to the Bishop of the Diocese of Freising after her death.

Line No. 2 dessert habitat areas such as: RESIDENCE UNIVERSITAIRE, STANILAS, PASTEUR, RESIDENCE DE BADUEL, and major traffic generators in the Greater Cayenne ( downtown and schools ). With a bus every 35 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 h beginning 15, 01 end 20 h.

* With unpaved runways: 15

He took office as the Prime Minister of India on 15 August, and delivered his inaugural address titled " A Tryst With Destiny "

With sacrifice and priesthood established, chapters 11 – 15 instruct the lay people on purity ( or cleanliness ).

With the growth of industrialism and the introduction of machinery, longer hours became far more common, with 14 – 15 hours being the norm, and 16 not at all uncommon.

* Sanger, David E., " War Figures Honored With Medal of Freedom ", The New York Times, December 15, 2004.

With liquid oxygen costing $ 0. 15 per kilogram and liquid hydrogen $ 2. 20 per kilogram, the Space Shuttle has a liquid propellant expense of approximately $ 1. 4 million for each launch that costs $ 450 million from other expenses ( with 40 % of the mass of propellants used by it being liquids in the external fuel tank, 60 % solids in the SRBs ).

With the conscript based system during mid 1995, Sweden consisted of 15 maneuver brigades and, in addition, 100 battalions of various sorts ( artillery, engineers, rangers, air defense, amphibious, security, surveillance etc.

With the arrival of Governor Cesare Maria De Vecchi on 15 December 1923, things began to change for that part of Somaliland.

With eleven free agents excluding Jason Schmidt who signed with the Dodgers for roughly $ 15 million a year, a new manager on board with Bruce Bochy coming from division rival San Diego, and the loss of veteran catcher Mike Matheny due to complications resulting from concussions sustained during his career, the Giants ' prospects for the 2007 season were less than favorable going into the winter off-season.

With just 15 seconds left in the half, Ray Wersching's ensuing squib kick was muffed by Bengals running back Archie Griffin, and the 49ers recovered the ball on the Bengals 4-yard line.

With the game almost completely out of reach, the Bills managed to respond with a 15 play, 79-yard drive to cut the score to 37 – 17 on a 2-yard touchdown pass from Kelly to Metzelaars.

With the aid of linebacker Levon Kirkland's 8-yard sack on Aikman, the Cowboys were forced to punt on their next drive and Pittsburgh regained possession of the ball at their own 32-yard line with 4: 15 remaining.

With 16 seconds left in the game, Bills kicker Steve Christie made what seemed to be the game-winning 41-yard field goal to give his team the lead, 16 – 15.

With respect to the issues, Dole promised a 15 % across-the-board reduction in income tax rates and made former Congressman and supply side advocate Jack Kemp his running mate.

With E =-9. 15 for the S-methyldibenzothiophenium ion, typical nucleophile values N ( s ) are 15. 63 ( 0. 64 ) for piperidine, 10. 49 ( 0. 68 ) for methoxide, and 5. 20 ( 0. 89 ) for water.

With of land and a population of only 333, 200 inhabitants ( 2010 est.

With 830, 000 inhabitants in the tri-national urban agglomeration as of 2004, Basel is Switzerland's second-largest urban area.

With a couple of hundred men, he subdued the local inhabitants and founded the city of Santiago de Nueva Extremadura, now Santiago de Chile, on February 12, 1541.

With the escalation of fighting in the Mexican Revolution, the regular resupply visits ceased, and the island's inhabitants were left to their own devices.

With an additional ten million inhabitants just outside the city, Cairo resides at the centre of the largest metropolitan area in Africa and the Arab World as well as the tenth-largest urban area in the world.

With 240, 191 inhabitants in the beginning of 2009, Ghent is Belgium's second largest municipality by number of inhabitants.

With only 24, 000 inhabitants in 1700, the population doubled about every 30 years.

With more than 20, 000 inhabitants Magdeburg was one of the largest cities in the Holy Roman Empire.

With its capital at Saarbrücken, it has an area of 2, 570 km² and ( as of 30 April 2012 ) 1, 012, 000 inhabitants.

With 101 inhabitants per square kilometre ( 262 / sq mi ), Slovenia ranks low among the European countries in population density ( compared to 402 / km² ( 1042 / sq mi ) for the Netherlands or 195 / km² ( 505 / sq mi ) for Italy ).

With over 5 million inhabitants, the greater Stuttgart Metropolitan Region is the fourth-biggest in Germany after the Rhine-Ruhr area, Berlin / Brandenburg and Frankfurt / Rhine-Main.

With a population of 500, 000 inhabitants ( as of 1 September 2011 ), and more than one million inhabitants including the suburbs, it is the third largest city of the Netherlands, after Amsterdam and Rotterdam.

With the water main severed and unable to extinguish the inferno, the inhabitants had no option but to flee to safety.

With about two million inhabitants, West Berlin had the highest number of residents of any city in Cold War-era Germany.

With more than 206, 000 inhabitants, it is the second largest city of Noord-Brabant, and the sixth city of the Netherlands.

With Goirle, it forms an agglomeration of 229, 000 inhabitants.

With 1, 202, 889 inhabitants as of 1 January 2008, the Toulouse metropolitan area is the fourth-largest in France, after Paris ( 12. 1 million ), Lyon ( 2. 1 million ), and Marseille ( 1. 7 million ).

With over 75 million inhabitants, it is the sixth largest state in India by population.

With its 323. 7 people per km < sup > 2 </ sup > on just over 12, 400 km < sup > 2 </ sup >, it is a densely populated region, having some four million inhabitants — seven percent of France's total population, making it the fourth most populous region in the country — 83 % of whom live in urban communities.

With 80, 194 inhabitants, Vichy's urban area is the second largest in the Auvergne region behind Clermont-Ferrand.

With the start of the Iran – Iraq War ( 1980 – 1988 ) following an Iraqi invasion, a second wave of inhabitants fled the city, especially during Iraqi air offensive on the capital.

With most major powers backing Iraq at that time, economic isolation caused even more reasons for the inhabitants to leave the city ( and the country ).