With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With a population of about 66, 000, Aalen is the 15 most-populated settlement in Baden-Württemberg.

With the Lombard invasion of Italy it was annexed to the Duchy of Benevento, until it was finally destroyed by the Byzantines of Constans II in 663, reducing to a small hamlet known as Quintodecimo ( referring to its distance of 15 miles from Benevento ).

With the conversion of Sergius Paulus, Paul begins to gain prominence over Barnabas from the point where the name " Paul ," his Roman name, is substituted for " Saul " ( 13: 9 ); instead of " Barnabas and Saul " as heretofore ( 11: 30 ; 12: 25 ; 13: 2, 7 ) we now read " Paul and Barnabas " ( 13: 43, 46, 50 ; 14: 20 ; 15: 2, 22, 35 ); only in 14: 14 and 15: 12, 25 does Barnabas again occupy the first place, in the first passage with recollection of 14: 12, in the last two, because Barnabas stood in closer relation to the Jerusalem church than Paul.

With the acquisition of Montagu House the first exhibition galleries and reading room for scholars opened on 15 January 1759.

With this deed dating back to August 15, 805 A. D., the Feast of the Assumption of the Blessed Virgin Mary, she donated her entire property in Dachau, including 5 so called Colonenhöfe and some serfs and bondsman, to devolve to the Bishop of the Diocese of Freising after her death.

Line No. 2 dessert habitat areas such as: RESIDENCE UNIVERSITAIRE, STANILAS, PASTEUR, RESIDENCE DE BADUEL, and major traffic generators in the Greater Cayenne ( downtown and schools ). With a bus every 35 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 h beginning 15, 01 end 20 h.

* With unpaved runways: 15

He took office as the Prime Minister of India on 15 August, and delivered his inaugural address titled " A Tryst With Destiny "

With sacrifice and priesthood established, chapters 11 – 15 instruct the lay people on purity ( or cleanliness ).

With the growth of industrialism and the introduction of machinery, longer hours became far more common, with 14 – 15 hours being the norm, and 16 not at all uncommon.

* Sanger, David E., " War Figures Honored With Medal of Freedom ", The New York Times, December 15, 2004.

With liquid oxygen costing $ 0. 15 per kilogram and liquid hydrogen $ 2. 20 per kilogram, the Space Shuttle has a liquid propellant expense of approximately $ 1. 4 million for each launch that costs $ 450 million from other expenses ( with 40 % of the mass of propellants used by it being liquids in the external fuel tank, 60 % solids in the SRBs ).

With the conscript based system during mid 1995, Sweden consisted of 15 maneuver brigades and, in addition, 100 battalions of various sorts ( artillery, engineers, rangers, air defense, amphibious, security, surveillance etc.

With the arrival of Governor Cesare Maria De Vecchi on 15 December 1923, things began to change for that part of Somaliland.

With eleven free agents excluding Jason Schmidt who signed with the Dodgers for roughly $ 15 million a year, a new manager on board with Bruce Bochy coming from division rival San Diego, and the loss of veteran catcher Mike Matheny due to complications resulting from concussions sustained during his career, the Giants ' prospects for the 2007 season were less than favorable going into the winter off-season.

With just 15 seconds left in the half, Ray Wersching's ensuing squib kick was muffed by Bengals running back Archie Griffin, and the 49ers recovered the ball on the Bengals 4-yard line.

With the game almost completely out of reach, the Bills managed to respond with a 15 play, 79-yard drive to cut the score to 37 – 17 on a 2-yard touchdown pass from Kelly to Metzelaars.

With the aid of linebacker Levon Kirkland's 8-yard sack on Aikman, the Cowboys were forced to punt on their next drive and Pittsburgh regained possession of the ball at their own 32-yard line with 4: 15 remaining.

With 16 seconds left in the game, Bills kicker Steve Christie made what seemed to be the game-winning 41-yard field goal to give his team the lead, 16 – 15.

With respect to the issues, Dole promised a 15 % across-the-board reduction in income tax rates and made former Congressman and supply side advocate Jack Kemp his running mate.

With E =-9. 15 for the S-methyldibenzothiophenium ion, typical nucleophile values N ( s ) are 15. 63 ( 0. 64 ) for piperidine, 10. 49 ( 0. 68 ) for methoxide, and 5. 20 ( 0. 89 ) for water.

With the launch of Operation Barbarossa on 22 June 1941, 6. Panzer took part in Army Group North's strike towards Leningrad, being involved in heavy fighting.

With casualties mounting and the Americans drawing closer, the German high command transferred the 3rd Panzergrenadier Division to Aachen, followed by the I SS Panzer Corps, which included the 116th Panzer Division and SS Heavy Panzer Battalion 101, an element of the 1st SS Panzer Division.

Guderian wrote From Normandy to the Ruhr: With the 116th Panzer Division in WWII ( The Aberjona Press, 2001 ) ISBN 0-9666389-7-2

With this unit, he served under Hermann Hoth ’ s Panzer Group 3 of the Army Group Centre in Operation Barbarossa, the invasion of the Soviet Union.

With a limited force of 90 tanks and two rifle regiments, four artillery regiments and elements of the 38th Rifle Division, he is credited with blunting the advance of Field Marshal von Bock's 7th Panzer, 17th Panzer and 20th Motorized Division at Vyazma and allowing numerous Soviet soldiers to escape encirclement.

With General Felix Steiner commanding the 11th SS Panzer Army, this was one of the last major German tank offensives of the war.

With just four M18 tank destroyers of the 705th Tank Destroyer Battalion to assist, the paratroopers attacked units of the 2nd Panzer Division, whose mission was to proceed by secondary roads via Monaville ( just northwest of Bastogne ) to seize a key highway and capture, among other objectives, fuel dumps — for the lack of which the overall German counter-offensive faltered and failed.

With the army now at Lake Ladoga and the city of Leningrad cut off, 1st Panzer Division formed part of the general attack against the city perimeter on September 8.

With about 3. 000 men its strength was well under the required for a Panzer Division ; however the situation required that it had to be scattered in several groups to support different sectors of the Eastern Front: Don, south of Kharkov, Hungarian 2nd Army, Italian 8th Army.

With these reinforcements, they were able to hold Pegasus Bridge against an attack by elements of the 21st Panzer Division, strongly supported by artillery.

With the southern flank on the Dniester, and the recent Soviet attacks in the north, the 1st Panzer Army was now in a salient.

With the loss of these reserves, any hope that may have been left of dealing a major defeat through offensive action to the II SS Panzer Corps ended.

With its flanks covered from both sides, the 2nd Mounted Rifles Regiment made a successful sortie towards the village of Kamieńsk, on September 4, where it surprised the German 4th Panzer Division, and destroyed several of its tanks and a dozen of its fuel tanks, killing approximately 100 enemy soldiers.

With the death of James III in 1488 at the Battle of Sauchieburn, his successor James IV successfully ended the quasi-independent rule of the Lord of the Isles, bringing the Western Isles under effective Royal control for the first time.

With the war approaching Moscow, Eisenstein was one of many filmmakers evacuated to Alma-Ata, where he first considered the idea of making a film about Czar Ivan IV.

* With the death of Duke Charles IV of Anjou, Anjou reverts to the French crown under Louis XI of France.

* August 4 – Thirty Years ' War: With the help of Danish and Swedish reinforcements, Stralsund is able to resist Wallenstein's siege until the landing of a Danish army, led by Christian IV of Denmark, forces Wallenstein to raise the siege and move his army to confront the new threat.

With the Treaty of Pavia in 1329, the emperor Louis IV, a son of Louis II, returned the Palatinate to his nephews Rudolf and Rupert.

With the exception of the Bishop of Augsburg, Henry IV received no support for his claims.

With the Opole Dukes the situation was more delicate: in 1287, Henry IV obtained the annulment of his marriage with their sister, who was sent back to her homeland.

* August 12 – With the help of the Genoese, Byzantine co-emperor Andronicus IV Palaeologus invades Constantinople and dethrones his father, John V Palaeologus, as co-emperor.

With Otto II busy surpressing revolts in Germany, the Venetians opposed to Pietro IV found their opportunity to depose him.

With the help of the Austrian Empire and allies, the Bonapartists were defeated in the Neapolitan War and Bourbon Ferdinand IV once again regained the throne and the kingdom.

With Constans ' death in 668, the throne passed to his son Constantine IV.

* With the aid of the Greek statesman and historian Polybius, the son of the former Seleucid king Seleucus IV Philopator, Demetrius escapes from Rome, where he has been held as a hostage for many years, and returns to Syria to claim the throne from his nephew Antiochus V. In the resulting dispute, Antiochus V and his regent, Lysias, are overthrown and put to death.

* With the guardians of the young king Ptolemy VI Philometor demanding the return of Coele-Syria to Egyptian control, the Seleucid king, Antiochus IV Epiphanes, decides on a preemptive strike against Egypt and invades the country, conquering all but the city of Alexandria.

* With Antiochus IV now absent from the country, the citizens of Alexandria choose Ptolemy VI's brother Ptolemy VIII Euergetes II as their king.

With the death of Bessus ( Artaxerxes IV ), Persian resistance to Alexander the Great ceases.

With Roxana giving birth to Alexander the Great's son, Alexander IV Aegus, a compromise is reached by the Macedonian generals so that he and Alexander the Great's young and dim-witted brother Philip III Arrhidaeus, are to be considered joint rulers.

With the Treaty of Bruzolo ( April 25, 1610 ), Charles Emmanuel allied with France against Spain, but the assassination of Henry IV changed the situation, as the treaty was not recognized by Marie de ' Medici, who immediately assumed regency for Henry's son Louis XIII, a minor.

With the help of Pope Urban IV Michael VIII concluded peace with his former enemies.

With Michael IV ’ s death in December 1041, Zoe adopted Michael ’ s nephew, who was crowned as Michael V. Although he promised to respect Zoe, he promptly banished her to a monastery on Princes ' Islands on charges of attempted regicide.

With Grifo contained, the two mayors, who had not yet proved themselves in battle in defence of the realm as their father had, on the initiative of Carloman, installed the Merovingian Childeric III as king ( 743 ), even though Martel had left the throne vacant since the death of Theuderic IV in 737.

With only his niece between himself and the throne, Philip engaged in some rapid political negotiations and convinced Charles of Valois, who along with Odo IV was championing Joan's rights, to switch sides and support him instead.

With the death of the childless Count Guy in 1308, his possessions in La Marche were seized by Philip IV of France.

With this intention in mind, he selected an Arshakuni named Artaxias IV ( Artashes ), a son of Vramshapuh, and made him King of Armenia.

* With Elizabeth of France ( 1603 – 1644, daughter of Henry IV of France ) — married 1615 at Burgos:

With the division of Arrighi sent in support of Davout, far on the right flank and the division of Saint-Sulpice detailed to protect Masséna's IV Corps, Bessières brought forward his only remaining unit, the mighty 1st heavy cavalry division, under the skilled 41-year-old general Nansouty.