With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With these completed and ice gone from the St. Peter's River ( present-day Minnesota river ) their 250 bushels of wheat, 100 bushels of oats and barley and 30 bushels of peas and some chickens were loaded onto the flat-bottomed boats and rowed up the river to Big Stone Lake, across into Lake Traverse, and down the Red.

With a population of about 30 million, it has an area of, making it the 42nd most populous and 41st largest nation in the world.

With capacity to produce up to 5, 000 engines a year by 100 specially trained personnel, like traditional Aston Martin engine production from Newport Pagnell, assembly of each unit is entrusted to a single technician from a pool of 30, with V8 and V12 variants assembled in under 20 hours.

With the win against the Boston Red Sox on September 30 and the loss of the Los Angeles Angels to the Texas Rangers in the second game of a double header, the Orioles clinched the playoff berth.

With the conversion of Sergius Paulus, Paul begins to gain prominence over Barnabas from the point where the name " Paul ," his Roman name, is substituted for " Saul " ( 13: 9 ); instead of " Barnabas and Saul " as heretofore ( 11: 30 ; 12: 25 ; 13: 2, 7 ) we now read " Paul and Barnabas " ( 13: 43, 46, 50 ; 14: 20 ; 15: 2, 22, 35 ); only in 14: 14 and 15: 12, 25 does Barnabas again occupy the first place, in the first passage with recollection of 14: 12, in the last two, because Barnabas stood in closer relation to the Jerusalem church than Paul.

With this issue alone, an estimated construction cost saving for a total of 58 office floors, would be around HK $ 30 million.

With almost 30 % of the total land area suitable for crop production and about 17 % of the labor force engaged in farming, agriculture remains the primary occupation, accounting for 11 % of GDP in 2001.

With over 30 million people worldwide today suffering Alzheimer's Disease each year, the study at UCLA helped discover that ongoing stimulation of the entorhinal cortex wasn't a necessary means to boost one's memory.

Line No. 1 dessert habitat areas such as: CHATENAY, HORTH, GRANT, COULEE D ’ OR, JARDIN DE ZEPHIR, BOURDA, COLIBRI, et ZEPHIR, and major traffic generators in the Greater Cayenne ( center city ​​ and administrative district schools ). With a bus every 30 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 5 h beginning 45, 20 end 14 h.

Line No. 5 serves residential areas such as: LES ALIZES, ANATOLE, BRUTUS, CESAIRE, EAU LISETTE, BONHOMME, CABASSOU, and major traffic generators in the town of Cayenne ( city, district administrative and schools ). With a bus every 30 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 pm start 00, end 20 h 04.

With this capstone, the National Constituent Assembly adjourned in a final session on 30 September 1791.

With strong endorsement and support from the Honduran military, the Suazo Administration ushered in the first peaceful transfer of power between civilian presidents in more than 30 years.

With President Bill Clinton's assistance, on 30 April 1994 Latvia and Russia signed a troop withdrawal agreement.

With the acquisition of new Ruthenian territories, this proportion decreased to 50 % and later to 30 %.

With an investment in excess of 30 million GBP, the Boots Advantage Card, launched in 1997, is / was the largest smart card retail loyalty card scheme in the world, and the third-largest retail loyalty scheme in the UK in terms of cards issued.

With only 24, 000 inhabitants in 1700, the population doubled about every 30 years.

With a 30 – 23 victory on Monday Night Football, the Vikings moved to a 4 – 0 record.

With neuronal heterogeneity ( by Edelman called degeneracy ), it is possible to test the many circuits ( on the order of 30 billion neurons with an estimated one quadrillion connections between them in the human brain ) with a diverse set of inputs, to see which neuronal groups respond " appropriately " statistically.

With the Empire's population reaching 30, 000, 000 people by 1600, shortage of land placed further pressure on the government.

With one card to come, Alice holds a made hand with little chance of improving and faces a $ 10 call to win a $ 30 pot.

With over 30, 000 of its army officers executed or imprisoned, including most of those of the highest ranks, the Red Army in 1939 had many inexperienced senior officers.

With its capital at Saarbrücken, it has an area of 2, 570 km² and ( as of 30 April 2012 ) 1, 012, 000 inhabitants.

With over four million American men away at war, Protestants forced their distinctive theological belief upon the general public.

With a membership currently estimated at over 85 million members worldwide, the Anglican Communion is the third largest Christian communion in the world, after the Roman Catholic Church and the Eastern Orthodox Churches.

With over 120 million visitors a year tourism is integral to the Alpine economy with much it coming from winter sports although summer visitors are an important component of the tourism industry.

With about 6 million native speakers in South Africa, or 13. 3 percent of the population, it is the third most spoken mother tongue in the country.

With a reserve ratio of 10 %, the bank can create 400 million USD in additional loans ( there is a time lag, and the bank has to expect to recover the loaned money back into its books ).

With a population of 3. 5 million people, Berlin is Germany's largest city and is the second most populous city proper and the seventh most populous urban area in the European Union.

With an annual production of approximately 960 million bottles, Bordeaux produces large quantities of everyday wine as well as some of the most expensive wines in the world.

With a $ 1. 92-billion-a-year tourism industry, Costa Rica stands as the most visited nation in the Central American region, with 1. 9 million foreign visitors in 2007, thus reaching a rate of foreign tourists per capita of 0. 46, one of the highest in the Caribbean Basin, and above other popular destinations such as Mexico ( 0. 21 ), Dominican Republic ( 0. 38 ), and Brazil ( 0. 03 ).

With over 10 million foreign tourists annually, tourism generates revenue in excess of € 7 billion.

With over 55, 000 missionaries worldwide, the church currently has a membership of over 14. 4 million and is ranked by the National Council of Churches as the fourth largest Christian denomination in the United States.

With a population of 6. 76 million spread over, Cairo is by far the largest city in Egypt.

With an additional ten million inhabitants just outside the city, Cairo resides at the centre of the largest metropolitan area in Africa and the Arab World as well as the tenth-largest urban area in the world.

With the aim of launching guerrilla operations against the Chinese, the CIA funded the Dalai Lama $ 1. 7 million a year in the 1960s.

With Dulles ' recommendation, he authorized the deployment of thirty Lockheed U-2's at a cost of $ 35 million.

With one third of the game ( 9 levels ) distributed as shareware, Doom was played by an estimated 10 million people within two years of its release, popularizing the mode of gameplay and spawning a gaming subculture ; as a sign of its effect on the industry, games from the mid-1990s boom of first-person shooters are often known simply as " Doom clones ".

With 2, 000 congregations and a membership of some 1. 8 million ( or 10. 8 % of the Dutch population, 2009 ), it is the second largest church in the Netherlands after the Roman Catholic Church.

With Danish help, Estonian crews began cleaning up the site, although they estimated the likely cost to be as much as 4 million EEK.

With a population of about 1. 3 million, Estonia has about 1. 7 million cellular telephones, 0. 5 million fixed phones, and 0. 8 million internet users ( as of 2006 ).

With 384 million internet users, China's online shopping sales rose to $ 36. 6 billion in 2009 and one of the reasons behind the huge growth has been the improved trust level for shoppers.