In all the above procedures, when the intermediate sera were diluted to 1: 10 or 1: 100 with 0.85% saline, the specific and nonspecific fluorescence were not appreciably reduced, whereas, a dilution of the intermediate sera to 1: 500 or diluting the Af to 1: 5 greatly reduced specific fluorescence.

More than 500 juniors and seniors are taking part in the program and 100 firms offer jobs on an educational rather than a need basis.

* Tungsten light bulb ( 60 – 100 W ): 500 – 830 mA

* A supply of play money, in denominations of $ 100, $ 500, $ 1000, and $ 5000

Founded in 1920 by Crystal Eastman, Roger Baldwin and Walter Nelles, the ACLU has over 500, 000 members and has an annual budget over $ 100 million.

Its subsidiary, American Media Operations Inc., listed assets of $ 100 to $ 500 million and debt of over $ 1 billion.

In 1990, Bulgaria had a total of more than 2, 400 tanks, 2, 000 armored vehicles, 2, 500 large caliber artillery systems, 300 fighter and bomber aircraft, 100 trainer aircraft, more than 40 combat and 40 transport helicopters, 4 submarine, 6 fast missile craft, 2 frigates, 5 corvettes, 6 torpedo boats, 9 patrol craft, 30 minesweepers and 21 transport vessels.

Bulgaria's total military personnel as of late 2010 is 34, 500, of which 30, 400 ( 88. 1 %) are active military personnel and 4, 100 ( 11. 9 %) are civilian personnel.

Compared to the 1991 figures this represents an overall increase of 137, 000, from 528, 500 ( out of a population of 2, 371, 100 ) fifteen years previously.

For example, a 500 gigabyte hard drive holds bytes, and a 100 megabit per second Ethernet connection transfers data at bit / s.

The population was rising ( estimates for Constantinople in the 12th century vary from some 100, 000 to 500, 000 ), and towns and cities across the realm flourished.

Each pit is approximately 100 nm deep by 500 nm wide, and varies from 850 nm to 3. 5 µm in length.

The range is typically 100 – 500 km, but can be greater.

Before World War I there were more than 3, 500 cheese producers in Britain, while fewer than 100 remained after the Second World War.

The Arithmometer, invented in 1820 as a four-operation mechanical calculator, was released to production in 1851 as an adding machine and became the first commercially successful unit ; forty years later, by 1890, about 2, 500 arithmometers had been sold plus a few hundreds more from two arithmometer clone makers ( Burkhardt, Germany, 1878 and Layton, UK, 1883 ) and Felt and Tarrant, the only other competitor in true commercial production, had sold 100 comptometers.

The first successful calculators with LCDs were manufactured by Rockwell International and sold from 1972 by other companies under such names as: Dataking LC-800, Harden DT / 12, Ibico 086, Lloyds 40, Lloyds 100, Prismatic 500 ( aka P500 ), Rapid Data Rapidman 1208LC.

Roman numerals have symbols for the decimal powers ( 1, 10, 100, 1000 ) and secondary symbols for half these values ( 5, 50, 500 ).

On Diego Garcia today, the military base uses over 100 shallow " horizontal " wells to produce over 560, 000 liters per day from the " Cantonment " lens on the northwest arm of the island-sufficient water for western-style usage for a population of 3, 500.

Basic, unpadded gig bags used by students cost under 100 USD, while thickly padded gig bags for professional players typically cost as much as 500 USD.

* maximum power: 2, 500 W ( of which 2, 100 W powers the ion thrust engine )

Statistics are not widely available on all sectors, but it was estimated that in 2003 – 4 there were 38, 100 main telephones lines, around 1, 050 Internet hosts, and 9, 500 Internet users.

The museum hosts a collection of approximately 1, 100 paintings, 4, 500 drawings, and 18, 000 prints, the broadest collection of his works in the world.

The recent Citadis-Dualis, intended to run at up to 100 km / h, is suitable for stop spacings ranging from 500 m to 5 km.

* 2001 – Inter-ethnic violence between Dayaks and Madurese breaks out in Sampit, Indonesia, that will ultimately result in more than 500 deaths and 100, 000 Madurese displaced from their homes.

Without Abkhazia and South Osetia, the population in the regions controlled by the central government of Georgia was 4, 321, 500 in 2005 and 4, 382, 100 in 2008 ( compare the 2008 figure with the CIA estimate of 4, 630, 841 for all of Georgia, including Abkhazia and South Osetia ).

Around 500 BCE, following the Achaemenid conquest of Mesopotamia under Darius I, Old Aramaic was adopted by the conquerors as the " vehicle for written communication between the different regions of the vast empire with its different peoples and languages.

* Kapila ( c. 500 BCE ), proponent of the Samkhya system of philosophy

* Pingala ( c. 500 BCE ), author of the Chandas shastra

Martin Noth was of the opinion that it dated from the 3rd century BCE, and Gary Knoppers, while acknowledging that Chronicles theoretically could be written anywhere between 500 and 250 BCE, tends to see it as probably dating between 325 and 275 BCE.

Trade with India commenced well before 500 BCE ( before the widespread use of Sanskrit as a language in India ).

In around 500 BCE, after the Zhou state weakened and China moved into the Spring and Autumn Period, the classic period of Chinese philosophy began ( it is an interesting fact that this date nearly coincides with the emergence of the first Greek philosophers ).

The various drums of taiko are of Chinese origin and were brought to Japan between the Yayoi period ( 500 BCE – 300 CE ).

Halakha has been developed and pored over throughout the generations since before 500 BCE, in a constantly expanding collection of religious literature consolidated in the Talmud.

The oldest depictions of harps without a forepillar are from 500 BCE, which was the Persian harp of Perspolis / Persia in Iran and from 400 BCE in Egypt.

In 500 BCE, the government of the Greek state of Sybaris offered one year's patent " to all who should discover any new refinement in luxury.

Natufian culture is commonly split into two subperiods: Early Natufian ( 12, 500 – 10, 800 BCE ) ( Christopher Delage gives a.

13, 700 to 11, 500 BCE ) and Late Natufian ( 10, 800 – 9500 BCE ).

Radiocarbon dating places the Natufian culture between 12, 500 and 9500 BCE, just before the end of the Pleistocene.

For the first time in the Indian Subcontinent, a continuous sequence of dwelling-sites has been established from 7000 BCE to 500 BCE, ( as a result of the ) explorations in Pirak from 1968 to 1974 ; in Mehrgarh from 1975 to 1985 ; and of Nausharo from 1985 to 1996.

Cattle were introduced to the Central Sahara ( Ahaggar ) from 4, 000 to 3, 500 BCE.

Remarkable rock paintings, many found in the Aïr Mountains, dated 3, 500 to 2, 500 BCE, portray vegetation and animal presence rather different from modern expectations.

The Kebaran culture ( 18, 000 – 10, 500 BCE ) not only introduced the microlithic assemblage into the region, it also has African affinity, specifically with the Ouchtata retouch technique associated with the microlithic Halfan culture of Egypt ( 24, 000 – 17, 000 BCE ).

The Kebarans in their turn were directly ancestral to the succeeding Natufian culture ( 10, 500 – 8500 BCE ), which has enormous significance for prehistorians as the clearest evidence of hunters and gatherers in actual transition to Neolithic food production.