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A restriction of 130 meals at any site being served to the hungry had for 18 years been an unenforced ordinance until March 2009, when a downtown developer presented data to the City Plan Board ( residents appointed by the City Commission to advise the Commission on planning issues ) showing that a downtown soup kitchen distributed significantly more meals than the legal limit, which resulted in a strict enforcement of the meal limit ordinance Outrage has engendered much activism, including the founding of the " Coalition to End the Meal Limit NOW!
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restriction and 130
Water restriction is needed if hyponatremia < 130 mmol per liter develops.
restriction and at
There is no restriction placed on the piece that is chosen on promotion, so it is possible to have more pieces of the same type than at the start of the game ( for example, two queens ).
However, this political restriction is less confining than it may first appear in that the Marxist historical framework is surprisingly flexible, and a rather simple matter to modify an alternative historical theory to use language that at least does not challenge the Marxist interpretation of history.
While most states require a for-profit corporation to have at least one director and two officers, Delaware laws do not have this restriction.
Wreen also considered a seventh requirement: "( 7 ) The good specified in ( 6 ) is, or at least includes, the avoidance of evil ", although as Wreen noted in the paper, he was not convinced that the restriction was required.
However, before this, some MPPC members got around this restriction by occasionally making longer stories in separate parts, and releasing them in successive weeks, starting with Vitagraph's The Life of Moses in five parts ( and five reels ) at the end 1909.
Prior to this, they were not limited as to how many terms they could serve ; John Engler, the governor at the time, served three terms as his first term occurred prior to the restriction.
The bill contained a restriction, that building would fall to two ships per year in 1912, but Tirpitz was confident of changing this at a later date.
The legal restriction that employers cannot pay less than a legislated wage is equivalent to the legal restriction that workers cannot work at all in the protected sector unless they can find employers willing to hire them at that wage.
Earlier in the year left-wing councils had protested at Government restriction of their budgets by refusing to set budgets, resulting in a budget crisis in Militant-dominated Liverpool City Council.
The gene to be replicated is inserted into copies of a plasmid containing genes that make cells resistant to particular antibiotics and a multiple cloning site ( MCS, or polylinker ), which is a short region containing several commonly used restriction sites allowing the easy insertion of DNA fragments at this location.
Some ritualize the act of prayer, requiring a strict sequence of actions or placing a restriction on who is permitted to pray, while others teach that prayer may be practiced spontaneously by anyone at any time.
A restriction enzyme ( or restriction endonuclease ) is an enzyme that cuts DNA at specific recognition nucleotide sequences ( with Type II restriction enzymes cutting double-stranded DNA ) known as restriction sites.
* Type I enzymes () cleave at sites remote from recognition site ; require both ATP and S-adenosyl-L-methionine to function ; multifunctional protein with both restriction and methylase () activities.
* Type II enzymes () cleave within or at short specific distances from recognition site ; most require magnesium ; single function ( restriction ) enzymes independent of methylase.
Similar to type IIE enzymes, type IIF restriction endonucleases ( e. g. NgoMIV ) interact with two copies of their recognition sequence but cleave both sequences at the same time.
Type IIS restriction endonucleases ( e. g. FokI ) cleave DNA at a defined distance from their non-palindromic asymmetric recognition sites.
These enzymes methylate only one strand of the DNA, at the N-6 position of adenosyl residues, so newly replicated DNA will have only one strand methylated, which is sufficient to protect against restriction.
In allele " A ", the genome is cleaved by a restriction enzyme at three nearby sites ( triangles ), but only the rightmost fragment will be detected by the probe.
Such larger leopards tend to be found in areas which lack tigers and lions, thus putting the leopard at the top of the food chain with no competitive restriction from large prey items.
restriction and any
The restriction to ZF renders any claim that relies on either the axiom of choice or its negation unprovable.
Schaefer's dichotomy theorem states that, for any restriction to Boolean operators that can be used to form these subformulae, the corresponding satisfiability problem is in P or NP-complete.
As it turned out, assuming that one can perform any operation on sets without restriction leads to paradoxes such as Russell's paradox and Berry's paradox.
Critical variables affecting this acoustic impedance include: chimney length ( hole between lip-plate and head tube ), chimney diameter, and radii or curvature of the ends of the chimney and any designed restriction in the " throat " of the instrument, such as that in the Japanese Nohkan Flute.
Social liberals and reformist, moderate non-socialists, however, opposed any restriction of parliamentarianism.
Such countries as Pakistan and Brazil have adopted the Codex Alimentarius Standard on Irradiated Food without any reservation or restriction: i. e., any food may be irradiated to any dose.
Although there is no obvious restriction that any given regular polygon cannot be a face of a Johnson solid, it turns out that the faces of Johnson solids always have 3, 4, 5, 6, 8, or 10 sides.
A free file format is a published specification for storing digital data not encumbered by any copyright, trademark, patent or other restriction.
The Montreal Protocol currently calls for a complete phase-out of HCFCs by 2030, but does not place any restriction on HFCs.
As mentioned above, in almost all competitive one-day games, a restriction is placed on the number of overs that may be bowled by any one bowler.
the term " racial discrimination " shall mean any distinction, exclusion, restriction, or preference based on race, colour, descent, or national or ethnic origin that has the purpose or effect of nullifying or impairing the recognition, enjoyment or exercise, on an equal footing, of human rights and fundamental freedoms in the political, economic, social, cultural or any other field of public life.
… any distinction, exclusion, restriction or preference based on race, color, descent, or national or ethnic origin that has the purpose or effect of nullifying or impairing the recognition, enjoyment or exercise, on an equal footing, of human rights and fundamental freedoms in the political, economic, social, cultural or any other field of public life.
Over time ground rules have been worked out and there haven't been any recent episodes of relationship restriction enforcement.
The NSG decided in 1992 to require full-scope IAEA safeguards for any new nuclear export deals, which effectively ruled out nuclear exports to India, but in 2008 waived this restriction on nuclear trade with India as part of the Indo-US civilian nuclear agreement.
Ischemia ( restriction of blood supply ) in the brain produces liquefactive, rather than coagulative necrosis due to the lack of any substantial supportive stroma.
In the end, this is not a serious restriction — any topological group can be made Hausdorff in a canonical fashion.
One advantage of this restriction is that the structures studied in universal algebra can be defined in any category which has finite products.
Clement IX ordered his nuncio to conduct a new investigation ' reporting back, the nuncio declared: " they have condemned and caused to be condemned the five propositions with all manner of sincerity, without any exception or restriction whatever, in every sense in which the Church has condemned them ".
Coincidentally, his beautiful young female secretary, Eunice Branca, is murdered, so her body is used, since Smith never thought to place any restriction on the sex of the donor.
Draining the intracellular reserves, on the other hand, is more challenging: moderate caloric restriction alone, even over a period of several months, appears to be ineffective, and it must be combined with physical exercise to have any effect.
restriction and site
Naturally occurring restriction endonucleases are categorized into four groups ( Types I, II III, and IV ) based on their composition and enzyme cofactor requirements, the nature of their target sequence, and the position of their DNA cleavage site relative to the target sequence.
Type I restriction enzymes possess three subunits called HsdR, HsdM, and HsdS ; HsdR is required for restriction ; HsdM is necessary for adding methyl groups to host DNA ( methyltransferase activity ) and HsdS is important for specificity of the recognition ( DNA-binding ) site in addition to both restriction ( DNA cleavage ) and modification ( DNA methyltransferase ) activity.
In allele " a ", restriction site 2 has been lost by a mutation, so the probe now detects the larger fused fragment running from sites 1 to 3.
Amplification can be directed across the altered restriction site, and the products digested with the restriction enzyme.
Recent phylogenetic studies based on cpDNA restriction site analysis and gene sequencing strongly suggest that the Cannabaceae sensu stricto arose from within the former Celtidaceae family, and that the two families should be merged to form a single monophyletic family, the Cannabaceae sensu lato.
* Multiple cloning site, the region of restriction enzyme sites in a plasmid
* SacI, a restriction enzyme with the restriction site GAGCTC
The mutation ( a 1691G → A substitution ) removes a cleavage site of the restriction endonuclease MnlI, so PCR, treatment with MnlI, and then DNA electrophoresis will give a diagnosis.
To allow for convenient and favorable insertions, most cloning vectors have had nearly all their restriction sites engineered out of them and a synthetic multiple cloning site ( MCS ) inserted that contains many restriction sites.
The Dominion's governor, Sir Edmund Andros, was highly unpopular in the colonies, but was especially hated in Massachusetts, where he angered virtually everyone by enforcing of the Navigation Acts, vacating land titles, appropriating a Puritan meeting house as a site to host services for the Church of England, and his restriction of town meetings, among other sundry complaints .< ref > Lovejoy, pp. 184 – 186,
cDNA molecules can be cloned by using restriction site linkers.
Linkers are short, double stranded pieces of DNA ( oligodeoxyribonucleotide ) about 8 to 12 nucleotide pairs long that include a restriction endonuclease cleavage site e. g. BamHI.
It involves the cleavage by a restriction enzyme at a site in the plasmid and subsequent ligation of a pair of complementary oligonucleotides containing the mutation in the gene of interest to the plasmid.
This method can generate mutants at close to 100 % efficiency, but is limited by the availability of suitable restriction sites flanking the site that is to be mutated.
This involves a single step of PCR, but still has the inherent problem of requiring a suitable restriction site near the mutation site unless a very long primer is used.
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