* Short-lived nature of gene therapy – Before gene therapy can become a permanent cure for any condition, the therapeutic DNA introduced into target cells must remain functional and the cells containing the therapeutic DNA must be long-lived and stable.

Before gene testing was available, the type and location of tumors determined which type of MEN2 a person had.

Before the Draft Act was passed Baker had confidentially briefed governors, sheriffs, and prospective draft board members on the administration of the measure -- and the confidence was kept so well that only one newspaper learned what was going on.

Before the fight was over, the Harlem boy had a concussion and Trig was cut up badly.

Before he left the apartment he knocked on their door and asked if there was anything he could do for them.

Before he left town Pat saw to it that I was fixed up with a job.

Before the airplanes came, he said, travel in Laos was just about impossible.

Before coming on a visit to Spelman in 1885, Miss Mary had been a successful teacher in Worcester, and her position there was held open for her for a considerable period.

Before he was forty Griffith had created the art of the film.

Before appealing to the U.N. or to Russia, he first appealed to the U.S. for military help, and was rejected.

Before her chore was finished she was rescuing wind-blown sheets of music, trundling microphones about the stage, helping to move the piano and otherwise joining in the informal atmosphere.

Before ASCII was developed, the encodings in use included 26 alphabetic characters, 10 numerical digits, and from 11 to 25 special graphic symbols.

Before Europeans discovered other oceans, the term " ocean " itself was synonymous with the waters beyond the Strait of Gibraltar that we now know as the Atlantic.

Before he could pass the river with safety, however, it was necessary to subdue certain fortresses, one of which was for several days vigorously defended by the governor, Yussuf el-Harezmi, a Khwarezmian.

Before the 1989 series began, the win-loss ratio was almost even, with 87 wins for Australia to England's 86, 74 having been drawn.

Before the late modern era, the abbot was treated with the utmost reverence by the brethren of his house.

Before the creation of the world, God was all by Himself, in a Self-absorbed state.

Before the Tennessee electorate voted on secession, Johnson, at his peril, toured the state, speaking in opposition to the measure, contending it was unconstitutional.

Before his death, he was absolved from the excommunication ; moreover, the Pope also promised that the King of Hungary and his relatives would not be excommunicated without the special permission of the Pope.

Before the 2007 season began, Vick was suspended indefinitely by the NFL after pleading guilty to charges involving dog fighting in the state of Virginia.

Before the War, Paul was a creative, sensitive, and passionate person, writing poems and having a clear love for his family.

Before the war Tjaden was a locksmith.

Before he can sell the secret of the atomic bomb to the Soviet Union, he is discovered by the British and is killed by members of MI5 who stage his death as a heart attack.

Before them, in 1784, the English chemist and physicist Henry Cavendish had discovered that air contains a small proportion of a substance less reactive than nitrogen.

Before the connection between magnetism and electricity was discovered, electrostatic generators were used.

Before the novel's opening, " hyper-assistance ", a technology allowing travel at a little slower than the speed of light, is used to move a reclusive space station colony called Rotor from the vicinity of Earth to the newly discovered red dwarf, Nemesis.

Before oil was discovered in Signal Hill, there were large homes built on the hill itself, and in the lower elevations was an agricultural area where fruits, vegetables, and flowers were grown.

Before Laurel became a city or a community, people passed through the site during the gold rush period, when gold was discovered at the Clarks Fork headwaters.

Before the Franks family could pay the ransom, Tony Minke, a Polish immigrant, discovered the body.

Before Alexander Fleming discovered penicillin, it was common in country districts for shepherds to apply this cheese to wounds in order to avoid gangrene.

Before the trial began, it was discovered that Honora Rieper had never married Herbert Rieper, the man known as her husband.

Before dying, Gurgenidze states that according to Madison's last transmission, the duo had discovered " something big ," and she had said that gaining proof could lead to war.

Many years later a second series, Before the Fact, originally published in 1901, was discovered and published in book form edited and introduced by detective fiction scholar Douglas G. Greene.

Before that, he jumps over a fence to get away, ending up looking in a window where a fat man is having rough sex with his lady, and when he is discovered, Kid runs away, and the three punks are shot at.

Before its discovery, the taxon was believed to have become extinct during the Miocene ; when it was discovered, it was heralded as a " living fossil ".

Before Alex can fully understand and dispute his induction, Xur appears ( via a holographic projection ) inside of the Starfighter base and reveals he has discovered an infiltrator in his ranks and proceeds to broadcast his death by torture to the entire base, including his father, Ambassador Enduran ( Kay E. Kuter ), the Starfighter commander.

Before leaving Italy, Carlo had already discovered a warbler new to science, the Moustached Warbler, and on the voyage he collected specimens of a new storm-petrel.

Before the technique of injecting air into molten iron was discovered by Kelly and by Bessemer, iron was available as cast iron, a strong but brittle metal made in a blast furnace by treating iron ore with coke derived from coal, and wrought iron, a more malleable and flexible metal made by heating iron ore in a low oxygen environment in a bloomery heated by charcoal and producing " blooms ", which were 100 to 200 pound lumps of very low carbon iron mixed with slag.

In 2009, a German film collector discovered a print of it and brought it to the attention of CBS / Paramount, which then released it under the title " Where No Fan Has Gone Before "-The Restored, Unaired Alternate Pilot Episode as part of the TOS season 3 box set on Blu-ray ; it has not yet been released on DVD.

Before the bad cheque is discovered, the perpetrator goes on a spending spree or obtains cash advances until the newly -" raised " available limit on the card is reached.

Before the 1990s historians could only speculate about its existence and content until in 1997, during a joint Finnish-Russian project, the Russian professor Oleg Rzesevski discovered the protocol in the Moscow Kremlin.

" Before, Richards stated " Further cracks appeared when Richards discovered that H had been secretly dating the band's manager Tim Byrne.

* February 27 — Before rescheduled launch, a piece of detached foam was discovered, which prompted a round of technical inspections.

Before the advent of telescopic photography, eight moons of Saturn were discovered by direct observation using optical telescopes.

Before the Naggarok was fully assimilated, the drives of the ship were destroyed by the crew, but the ship released a distress beacon, which the Kuun-Lan discovered.