When 1 ml of conjugate was passed through a column ( Af ), the first and second milliliter fractions collected were the most specific and gave no nonspecific staining in some experiments, and very little in others.

The second bottle passed a few times.

After a long debate in the House, a second attempt passed Congress on January 13, 1865, and was sent to the state legislatures for ratification.

As he passed where I was standing, he raised his head for a second, and to this day I can remember what I felt as our eyes met.

The moderates passed the Freedmen's Bureau Act a second time, and again the president vetoed it, but on this occasion the veto was overridden.

The doctrine was formulated in the second century in the first of the three senses given by Ramsey, originally as a response to Gnostic claims of having received secret teaching from Christ or the apostles ; it emphasised the public manner in which the apostles had passed on authentic teaching to those whom they entrusted with the care of the churches they founded and that these in turn had passed it on to their successors.

They would draw and fire with their strong hand, and when they had emptied the first gun, they drew the second gun with their weak hand and passed it over to their strong hand.

For example, a runner on first base advances to second on a passed ball.

Since the runner would now have been at second anyway, the passed ball no longer has any impact on the earned / unearned calculation.

In the second year of the Peloponnesian War ( 430 BC ), Thucydides described an epidemic disease which was said to have begun in Ethiopia, passed through Egypt and Libya, then come to the Greek world.

During a second voyage in 1494, Columbus passed along the south coast of the island, landing at various inlets including what was to become Guantánamo Bay.

Then it passed through the oval Forum of Constantine where there was a second Senate-house and a high column with a statue of Constantine himself in the guise of Helios, crowned with a halo of seven rays and looking toward the rising sun.

Now ( from 10 December 2007 ), in order of Italian legislation system, the law is before the House and has to be passed also by the Camera dei Deputati, the second house of Italian Parliament, to become an effective law.

The second point of transfer was near the Caprivi Strip and the Okavango River, where it was apparently the Yeyi language which borrowed the clicks from a West Kalihari Khoe language, and which in turn passed on a reduced click inventory to the neighboring Mbukushu, Kwangali, Gciriku, Kuhane, and Fwe languages in Angola, Namibia, Botswana, and Zambia.

The second run was even faster ; as K7 passed the start of the measured kilometre, she was travelling at over.

He was awarded a doctorate of jurisprudence, and then in 1971 passed the second state examination with distinction.

In a manner similar to the bread, the priests say the second prayer and the water is then passed to the congregation.

The coulomb is defined as the quantity of charge that has passed through the cross section of an electrical conductor carrying one ampere within one second.

On his death the titles passed to his eldest son from his second marriage, the third Earl.

When he died the titles passed to his eldest son from his second marriage to Harriet Douglas, the fifth Earl.

The surname passed to the descendants of Fujiwara no Fuhito ( 659 – 720 ), the second son and heir of Kamatari, who was prominent at the court of several emperors and empresses during the early Nara period.

Earlier in December 2005, the European Commission initiated the second stage of infringement proceedings against the British Government relating to Gibraltar ’ s failure to transpose five European Union directives on electronic communications, but these were closed after the relevant legislation was passed into law by the House of Assembly in June 2006.

However, not long after the second performance, the opera became so successful that the fame of Paisiello's opera was transferred to Rossini's, to which the title The Barber of Seville passed as an inalienable heritage.

At war's end leadership in Western Europe passed from Britain because the Labour Government devoted its attention to the creation of a welfare state.

The five enterprises which passed to 100 percent Russian state ownership are:

In his first biennial speech, he urged simplification of the state judicial system, abolishment of the Bank of Tennessee and establishment of an agency to provide uniformity in weights and measures, the latter of which was passed.

The state legislature had passed resolutions instructing their representatives in Washington to support pro-slavery and popular sovereignty measures such as the LeCompton Constitution and the Kansas – Nebraska Act.

In January 1864 Johnson organized a gathering of his state's Union loyalists, where resolutions were passed to elect county officials throughout the state, including a plan for a convention to dispose of the slavery issue ; also adopted was a very controversial and mandatory oath for voters, to protect and preserve the Union in the future.

The state governments installed by Johnson all passed Black Codes that gave the freedmen subordinate legal status.

In early March Congress, led in part by Radical Republicans, passed the first in a series of four Reconstruction Acts, initially providing for the recognition of provisional governments to be established thereunder by the Southern states, on the condition that each state ratify the Fourteenth Amendment and assure suffrage for freedmen.

By the 1920s, each state in the United States had passed public laws that stipulated a certain day to be Arbor Day or Arbor and Bird Day observance.

Prior to the passage of the United States Constitution, several States passed their own various copyright laws between 1783 and 1787, the first state being Connecticut.

Long-time President Houphouët Boigny, in power since the 1960s, passed away in December 1993, unleashing a succession crisis which quickly involved the power institutions of the state.

In 1990, it was discovered in the archive of the Stasi ( the state security service of the former German Democratic Republic ) that a member of CND's governing council, Vic Allen, had passed information to them about CND.

City, and later state laws, that upgraded standards for the medical profession and fought urban epidemics of cholera, small pox, and yellow fever were not only passed, but also enforced.

In March 2007, Virginia passed a law similar to CIPA that requires public libraries receiving state funds to use content-control software.

* 1905 – In France, the law separating church and state is passed.

In 1967 he passed the state law exam and then worked at the University of Regensburg in criminal law and Eastern European law.

* 2009: The Senate of Brazil passed a bill which would make Esperanto an optional part of the curriculum in its state schools.

* 1998 – Voters in Maine repeal a gay rights law passed in 1997 becoming the first U. S. state to abandon such a law.

The Civil Constitution of the Clergy, passed on 12 July 1790, turned the remaining clergy into employees of the state.

The governor has a duty to enforce state laws, and the power to either approve or veto bills passed by the New York State Legislature, to convene the legislature, and to grant pardons, except in cases of treason and impeachment.

Upon Hindenburg's death on 2 August 1934, Hitler's cabinet passed a law proclaiming the presidency vacant and transferred the role and powers of the head of state to Hitler as Führer und Reichskanzler ( leader and chancellor ).

Most countries require that all bills passed by the house or houses of the legislature be signed into law by the head of state.

However, in most parliamentary systems, the head of state cannot refuse to sign a bill, and, in granting a bill their assent, indicate that it was passed in accordance with the correct procedures.