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If embryonic stem cells are to be used for gene targeting to make a chimera, the following procedure is common: a construct for homologous recombination for the gene targeted will be introduced into cultured mouse embryonic stem cells from the donor mouse, by way of electroporation ; cells positive for the recombination event will have antibiotic resistance, provided by the insertion cassette used in the gene targeting ; and be able to be positively selected for.
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If and embryonic
If chaetognaths branched off from the protostomes before they evolved their distinctive protostome embryonic characters, they may have retained deuterostome characters inherited from early bilaterian ancestors.
If so, embryonic stem cells are genetically transformed and inserted into early embryos.
If they are given the right chemical environment these cells have the same ability as embryonic stem cells to develop into many different cell types.
If and stem
If word classes differ in their resistance or liability to stem replacement within meaning slot, it is conceivable that individual meanings also differ with fair consistence trans-lingually.
If no cruel hand pluck me from my stem, yet I must perish by an early doom.
If the handle is circular with the stem as the axis of rotation in the center of the circle, then the handle is called a handwheel.
If many bracts are present and they are strictly connected to the stem, like in the family Asteraceae, the bracts might collectively be called an involucre.
If the inflorescence has a second unit of bracts further up the stem, they might be called an involucel.
If successful, human egg donations would not be needed to create custom stem cell lines.
In this way, a therapist can facilitate the generation of awareness and insight ( for example, with a stem such as, “ If my fear could speak, it might say —”), as well as shifts in cognitive-motivational structure ( for example, with a stem such as, “ If I were to be kinder to myself when I ’ m afraid —”).
If a pressure gauge is attached to the cut stem, the root pressure can be measured
If Pe ‘ or is connected to the Hebrew stem p ‘ r ' open ', used both of mouth and bowels, it might mean ' opening ' and so Ba ‘ al Pe ‘ or could mean ' Lord of the Opening '.
If indeed some cancer stem cells use an alternative method of telomere maintenance, it should be noted that they are still killed when the RNA template of telomerase is blocked.
Altshuller believed that inventive problems stem from contradictions ( one of the basic TRIZ concepts ) between two or more elements, such as, " If we want more acceleration, we need a larger engine ; but that will increase the cost of the car ," that is, more of something desirable also brings more of something less desirable, or less of something else also desirable.
If this is confirmed, it would mean the sea spiders are the last surviving ( and highly modified ) members of an ancient stem group of arthropods that lived in Cambrian oceans.
If the source of auxin is removed, for example the tips of stems are trimmed, the roots are less stimulated accordingly, and growth of stem is supported instead.
If short syllable with yat in the word stem lengthens due to the phonetic or morphological conditions, reflex of is preserved, e. g. djȅlo – djȇlā, nèdjelja – nȅdjēljā.
If the noun or adjective has two vowel stems, the strong vowel stem comes from the essive singular.
If the flower stem is cut without flowering, a sweet liquid called agua miel (" honey water ") gathers in the heart of the plant.
If I get ill, she can cure the jeweled stem.
If the group G is finite and one considers only stem extensions, then there is a largest size for such a group C, and for every C of that size the subgroup K is isomorphic to the Schur multiplier of G. If the finite group G is moreover perfect, then C is unique up to isomorphism and is itself perfect.
If and cells
If Af leads to an address Af that is equal to the address computed from Af, even though Af does not match Af, the chain of information cells is extended from Af by storing the address of the next available cell in the Y-region, Af, in Af.
If cell Af is an information cell, it and any information cells in the Y-region that have been linked to Af each contain an address in the W-region where a potentially matching form is stored.
If activated cytotoxic CD8 < sup >+</ sup > T cells recognize them, the T cells begin to secrete various toxins that cause the lysis or apoptosis of the infected cell.
If the traffic on a virtual circuit is exceeding its traffic contract, as determined by the GCRA, the network can either drop the cells or mark the Cell Loss Priority ( CLP ) bit ( to identify a cell as potentially redundant ).
If present, clue cells can be visualized under a microscope.
If hypertonic therapy continues or is too rapid, the extracellular tonicity will continue to drive water out of the brain's cells, leading to cellular dysfunction and the condition of central pontine myelinolysis.
Among the forefathers of the new journalism movement, Thompson said in the February 15th, 1973 issue of Rolling Stone, " If I'd written the truth I knew for the past ten years, about 600 people — including me — would be rotting in prison cells from Rio to Seattle today.
If oxygen delivery to cells is insufficient for the demand ( hypoxia ), hydrogen will be shifted to pyruvic acid converting it to lactic acid.
If DNA was the genetic material, then DNA from the labeled phage would have to enter the bacterial cells, and carry the radioactive phosphorus with it.
If the glucose inside the cells is high, the cells will convert it to the insoluble glycogen to prevent the soluble glucose from interfering with cellular metabolism.
If the voltage changes by a large enough amount, an all-or-none electrochemical pulse called an action potential is generated, which travels rapidly along the cell's axon, and activates synaptic connections with other cells when it arrives.
If the time period of blood supply impairment lasts more than a few minutes, the nerve cells of that area of the brain die and cause permanent neurologic damage.
If one of these atypical sperm cells contributes to the genetic makeup of a child, the child will have an extra Y-chromosome in each of the body's cells.
If a second threshold ( E < sub > ir </ sub >) is reached or surpassed, electroporation will compromise the viability of the cells, i. e., irreversible electroporation.
If growth is not limited, doubling will continue at a constant rate so both the number of cells and the rate of population increase doubles with each consecutive time period.
* If lead-acid batteries are used, all cells in the formerly good parallel strings will begin to sulfate due to the inability for them to be fully recharged, resulting in the storage capacity of these cells being permanently damaged, even if the damaged cell in the one degraded string is eventually discovered and replaced with a new one.
If absent or deficient of natural tissue, alternatives can be cultured sheets of epithelial cells in vitro or synthetic compounds, such as integra, which consists of silicone and bovine tendon collagen with glycosaminoglycans.
* Root pressure: If the water potential of the root cells is more negative than that of the soil, usually due to high concentrations of solute, water can move by osmosis into the root from the soil.
If the collected sample contains too few sperm cells to likely have a spontaneous fertilization, intracytoplasmic sperm injection can be performed.
If the producing tissue is removed, the signal ceases and body cells revert to normal insulin sensitivity.
If cells divided without telomeres, they would lose the ends of their chromosomes, and the necessary information they contain.
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