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Once and vesicles
Once in the cytoplasmic space, tyramine will be transported via the vesicular monoamine transporter ( VMAT ) into synaptic vesicles, thereby displacing norepinephrine.
Once back in the cytosol, it is subsequently repackaged into vesicles by VMAT2.
Once the vesicles reach their targets, they come into contact with tethering factors that can restrain them.
Once endocytic vesicles have uncoated, they fuse with early endosomes.
Once opsonized, the clathrin-coated vesicle uncoats ( a pre-requisite for the vesicle to fuse with other membranes ) and individual vesicles fuse to form the early endosome.

Once and have
Once in a while they said what a shame it was, with Granny dying, but they all agreed she wouldn't have wanted it any other way.
Once we have made clear that we are genuinely concerned with a country's development potential, we can be blunt in suggesting the technical conditions that must be met for development to occur.
Once the children have been commodified, Swift ’ s rhetoric can easily turn " people into animals, then meat, and from meat, logically, into tonnage worth a price per pound ".
Once he has received this blessing, the abbot not only becomes father of his monks in a spiritual sense, but their major superior under canon law, and has the additional authority to confer the ministries of acolyte and lector ( formerly, he could confer the minor orders, which are not sacraments, that these ministries have replaced ).
Once these projects have been approved by the United States Air Force or other agencies such as the CIA, and are ready to be announced to the public, operations of the aircraft are then moved to a normal air force base.
Once images have been acquired, the effects of decay and taphonomy must be accounted for before a correct anatomical reconstruction can be made.
Once the governing party-the National Movement Simeon II have not amassed enough votes to enter the parliament.
Once players have mastered these basic strokes, they can hit the shuttlecock from and to any part of the court, powerfully and softly as required.
CD-R is a Write Once Read Many ( WORM ) optical medium, although the whole disk does not have to be entirely written in the same session.
Once ten yards have been gained the offence gains a new set of three downs ( rather than the four downs given in American football ).
Once the team have come back empty handed, he argues with Scott for not telling him about the team he deployed to find former Acolytes.
Once all appeals have been exhausted on a case, the judgment is final and the action of the prosecution is closed ( code of penal procedure, art.
Once the High Lamas have found the home and the boy they believe to be the reincarnation, the boy undergoes a series of tests to affirm the rebirth.
Once these basic shapes have been assembled into a likeness, then the drawing can be refined into a more accurate and polished form.
Once the particles have accelerated to the required energies, separate electron and positron beams are brought into collision.
Once the onions have softened, niter kebbeh ( or, in the case of vegan dishes, vegetable oil ) is added.
Once trees have been removed by fire or logging, infiltration rates become high and erosion low to the degree the forest floor remains intact.
Once her sons had left for Paris, Eleanor may have encouraged the lords of the south to rise up and support them.
Once, Muhammad met a group of soldiers returning from a defensive battle and addressed them: " Welcome to the people who have concluded the minor jihad ( struggle ).
Once in the arena morgue, the corpse would have been stripped of armour, and probably had its throat cut to prove that dead was dead.
Once the actors are onboard the Protector, they finally realize the truth, but Sarris prevents them from leaving, so they assume their television roles in order to save the Thermians, who have very little concept of deception, or the art of acting / theater.
Nevertheless, Wells has this very same Time Traveller speak in terms unusual for socialist thought, referring as " perfect " and with no social problem unsolved, to an imagined world of stark class division between the rich assured of their wealth and comfort, and the rest of humanity assigned to lifelong toil: Once, life and property must have reached almost absolute safety.
Once the roots have reached the reservoir below, there is not a huge advantage with Bubbleponics over Deep Water Culture.
Once ignited and burnt, the combustion products — hot gases — have more available thermal energy than the original compressed fuel-air mixture ( which had higher chemical energy ).
Once he was done delivering the speech, everyone across the U. S. knew of the new AFL-CIO whose " mission to bring social and economic justice to our nation by enabling working people to have a voice on the job, in government, in a changing global economy and in their communities.

Once and docked
Once there, they successfully located the Maquis ship docked at the Caretaker's array.
Once at the station, Canadarm2 removed Columbus from the docked shuttle's cargo bay
Once docked, it marked the first time that three spacecraft were docked together.
Once it is established that docking their stingers renders them harmless, docked triffids soon become fashionable in public and private gardens.
Once the two elements were docked, Ross and Newman conducted two scheduled spacewalks to connect power and data cables between the Node, PMAs and the FGB.
Once an insulin molecule has docked onto the receptor and effected its action, it may be released back into the extracellular environment or it may be degraded by the cell.
Once the countdown until detonation has begun, Cortana directs the Chief to a fighter still docked in the Pillar of Autumn hangar.
Once the ship was docked, the captain set about stalling the authorities for as long as possible with excuses, which further convinced the authorities that they had intercepted the real cargo.
Once assembled, the combined vehicle would offer the benefits of enhanced operating space for the crew, along with the traditional necessities of the ' hard ' spacecraft docked to it, such as atmospheric re-entry.
Once docked, the engines of the Kosmos were used to make orbital changes for the Salyut station.

Once and with
Once more he lifted Jess's gun from its holster, only this time he tossed it into the stall with the frightened buckskin.
Once covertly looking at Simms Purdew, the only man in the world whom he hated, he had seen the heavy, slack, bestubbled jaw open and close to emit the cruel, obscene banter, and had seen the pale-blue eyes go watery with whisky and merriment, and suddenly he was not seeing the face of that vile creature.
Once her trembling hand, with the pen grasped tight in it, was pressed against the paper the words came sharply, smoothly, as authoritatively as they would dropping from her own lips.
Once more and roared off into the fog, his foot evidently surprising him with the suddenness with which it pressed the accelerator, just as his hand did when he worked.
Once you become an avocado fan, you will look forward to the season each year with eager anticipation.
Once there, the D.A. with devilish cleverness would provide Marshall with headlines: `` Viola's Multiple Romances '' `` Viola Lake an Addict '' `` Downfall of Another Film Idol ''!!
Once he has been identified, however, a new melody is used to accompany his narrative, a bleak motif with barren octaves creating a rather ancient effect:
Once, in New York, he had gone flying with some friends in a small private airplane with a single engine.
Once inside his apartment, he poured a drink with trembling hands and flopped limply in a chair.
Once Pan had the audacity to compare his music with that of Apollo, and to challenge Apollo, the god of the kithara, to a trial of skill.
Once programmed, it is able to choreograph its motions and gestures with its voice.
Once chosen, he must request blessing: the blessing of an abbot is celebrated by the bishop in whose diocese the monastery is or, with his permission, another abbot or bishop.
Once set, the anchor tends to break out and reset when the direction of force changes dramatically, such as with the changing tide, and on some occasions it might not reset but instead drag.
Once inside the fortification, Alfred realised, the Danes enjoyed the advantage, better situated to outlast their opponents or crush them with a counter attack as the provisions and stamina of the besieging forces waned.
Once the game ends, the minority and majority bonuses are paid to the minority and majority holders in each of the remaining chains ; each player sells his or her shares of stock in each of the remaining chains ; and the player with the most money wins.
Once inside, they pressurized the LM cabin, went through a half-hour briefing with scientists in mission control, and configured the cabin for the sleep period.
Once the operator took his finger off, the player would speed up until its tachometer was back in phase with the master, and as this happened, the phasing effect would appear to slide up the frequency spectrum.
Once cytochrome c is released it binds with Apoptotic protease activating factor-1 ( Apaf-1 ) and ATP, which then bind to pro-caspase-9 to create a protein complex known as an apoptosome.
Once again Abd al-Rahman had to be concerned with developments in the Upper March.
Once the tip penetrates the animal the upper sub head broke off from the rest of the shaft, however, since it was still connected with the braided loop it rotated the head into a horizontal position inside the animal ’ s body so that it could not get away from the hunter.

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