[permalink] [id link]
# REDIRECT Extracorporeal membrane oxygenation
from
Wikipedia
Some Related Sentences
# and REDIRECT
# and Extracorporeal
A Extracorporeal shock wave lithotripsy | lithotriptor machine is seen in an Operating theater | operating room ; other equipment is seen in the background, including an Anaesthetic machine | anesthesia machine and a X-ray image intensifier # Mobile Fluoroscopic System AKA " portable C-arm " | mobile fluoroscopic system ( or " C-arm ").
# and membrane
1-channel protein domain | domain s ( typically four per channel ), 2-outer vestibule, 3-Potassium channel # Selectivity filter | selectivity filter, 4-diameter of selectivity filter, 5-phosphorylation site, 6-cell membrane.
# The DNA passes through a separate sugar transport protein ( ptsG ) in the inner membrane, and immediately circularises using the cos sites, 12-base G-C rich cohesive " sticky ends ".
# If some of the DNA fragments are larger than 15 kb, then prior to blotting, the gel may be treated with an acid, such as dilute HCl, which depurinates the DNA fragments, breaking the DNA into smaller pieces, thus allowing more efficient transfer from the gel to membrane.
# A sheet of nitrocellulose ( or, alternatively, nylon ) membrane is placed on top of ( or below, depending on the direction of the transfer ) the gel.
# The membrane is then baked in a vacuum or regular oven at 80 ° C for 2 hours ( standard conditions ; nitrocellulose or nylon membrane ) or exposed to ultraviolet radiation ( nylon membrane ) to permanently attach the transferred DNA to the membrane.
# The membrane is then exposed to a hybridization probe — a single DNA fragment with a specific sequence whose presence in the target DNA is to be determined.
# After hybridization, excess probe is washed from the membrane ( typically using SSC buffer ), and the pattern of hybridization is visualized on X-ray film by autoradiography in the case of a radioactive or fluorescent probe, or by development of color on the membrane if a chromogenic detection method is used.
# External limiting membrane – layer that separates the inner segment portions of the photoreceptors from their cell nucleus
0.102 seconds.