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RecA binds to DNA-bound LexA causing LexA to cleave itself in a process called autoproteolysis.
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RecA and binds
# Normally RecA * binds LexA ( a transcription repressor ), activating LexA auto-protease activity, which destroys LexA repressor allowing production of DNA repair proteins.
In the initiation step, RecA protein binds to ssDNA in an ATP hydrolysis driven reaction creating RecA – ssDNA filaments.
Once the DNA damage is repaired or bypassed using polymerases or through recombination, the amount of single-stranded DNA in cells is decreased, lowering the amounts of RecA filaments decreases cleavage activity of LexA homodimer, which then binds to the SOS boxes near promoters and restores normal gene expression.
The RecA protein binds strongly and in long clusters to ssDNA to form a nucleoprotein filament.
RecA and LexA
The RecA protein, stimulated by single-stranded DNA, is involved in the inactivation of the LexA repressor thereby inducing the response.
The activated form of RecA interacts with the LexA repressor to facilitate the LexA repressor's self-cleavage from the operator.
The prokaryotic SOS system is regulated by two key proteins: LexA and RecA.
RecA – ssDNA filaments activate LexA autoprotease activity, which ultimately leads to cleavage of LexA dimer and subsequent LexA degradation.
LexA is intimately linked to RecA in the biochemical cycle of DNA damage and repair.
This offers potential for combination therapy that combine quinolones with strategies aimed at interfering with the action of LexA either directly, or via RecA.
RecA and process
Triple-stranded DNA was also described in 1957, when it was thought to occur in only one in vivo biological process: as an intermediate product during the action of the E. coli recombination enzyme RecA.
RecA and .
# RecA ( a cellular protein ) detects DNA damage and becomes activated.
It is now RecA *, a highly specific co-protease.
In lysogenic cells this response is hijacked, and RecA * stimulates cI autocleavage.
Susceptible to cleavage by RecA * in cells undergoing the SOS response.
The latter is determined solely by the activation of RecA in the SOS response of the cell, as detailed in the section on induction.
RecA forms a filament around these ssDNA regions in an ATP-dependent fashion, and becomes activated.
Eighteen of these conserved indels in important housekeeping proteins such as Pol1, RecA, TrpRS and ribosomal proteins L4, L7 / L12, S8, S9, etc.
RecA is a 38 kilodalton Escherichia coli protein essential for the repair and maintenance of DNA.
A RecA structural and functional homolog has been found in every species in which one has been seriously sought and serves as an archetype for this class of homologous DNA repair proteins.
RecA has multiple activities, all related to DNA repair.
The RecA protein catalyzes unidirectional branch migration and by doing so makes it possible to complete recombination, producing a region of heteroduplex DNA that is thousands of base pairs long.
Since it is a DNA-dependent ATPase, RecA contains an additional site for binding and hydrolyzing ATP.
RecA associates more tightly with DNA when it has ATP bound than when it has ADP bound.
E. coli strains deficient in RecA are useful for cloning procedures in molecular biology laboratories.
Without functional RecA protein, the exogenous plasmid DNA is left unaltered by the bacteria.
binds and LexA
Under normal conditions, LexA binds to a 20-bp consensus sequence ( the SOS box ) in the operator region for those genes.
The LexA homodimer is a transcriptional repressor that binds to operator sequences commonly referred to as SOS boxes.
binds and causing
Deforestation causes increased erosion rates due to exposure of mineral soil by removing the humus and litter layers from the soil surface, removing the vegetative cover that binds soil together, and causing heavy soil compaction from logging equipment.
The phage particle recognizes and binds to its host, E. coli, causing DNA in the head of the phage to be ejected through the tail into the cytoplasm of the bacterial cell.
The p21 ( WAF1 ) protein binds directly to cyclin-CDK complexes that drive forward the cell cycle and inhibits their kinase activity thereby causing cell cycle arrest to allow repair to take place.
When lactose is present, allolactose binds to the lac repressor, causing an allosteric change in its shape.
When lactose is present, it binds to the repressor, causing it to detach from the DNA strand.
It is thought that 2C-E binds to the 5-HT2A receptor, causing its intoxicating effects.
When cells are grown in the presence of lactose, however, a lactose metabolite called allolactose, which is a combination of glucose and galactose, binds to the repressor, causing a change in its shape.
When glutamate binds to AMPARs located on the postsynaptic membrane, they permit a mixed flow of Na < sup >+</ sup > and K < sup >+</ sup > to cross the cells membrane, causing a depolarization of the postsynaptic membrane.
The hapten binds up antibodies toward that molecule without causing the immune response, leaving less antibodies left to bind to the molecule itself.
The cell then releases IL-2, which binds to its own new IL-2 receptors, causing self-stimulation and ultimately a monoclonal population of T cells.
CD134 is predominately found on activated T cells and binds to OX40 ligand causing T-cell stimulation, proliferation, activation, and apoptosis ( 3 ).
Bromazepam binds to the GABA receptor GABA < sub > A </ sub >, causing a conformational change and increasing inhibitory effects of GABA.
However, the great stone snake which binds Loki drips acid onto his gaping chest wound, causing him to scream with rage.
The mechanism for joining glucose units is that glycogen synthase binds to UDPG, causing it to break down into an oxonium ion, also formed in glycogenolysis.
When the person again encounters the allergen, it binds to the IgE that has already attached to basophils and mast cells, causing release of histamine, prostaglandins, and leukotrienes, producing inflammation of the surrounding tissues, and bringing about the familiar allergic symptoms.
Meprobamate binds to GABA < sub > A </ sub > receptors which interrupts neuronal communication in the reticular formation and spinal cord, causing sedation and altered perception of pain.
The egg yolk contains more than enough levels of biotin to compensate for the high levels of avidin in raw egg white, which binds to the B-vitamin biotin, preventing their absorption and potentially causing a deficiency if the yolk is not consumed with the white.
Virus internalisation depends on an Arginine-Glycine-Asparagine ( RGD ) motif at the base of adenovirus coat protein that binds to integrins, causing endocytosis.
When the fleshy part of the fruit is eaten, this molecule binds to the tongue's taste buds, causing sour foods to taste sweet.
* Quinupristin binds to a nearby site on the 50S ribosomal subunit and prevents elongation of the polypeptide, as well as causing incomplete chains to be released.
He is eventually destroyed for good when Metalder binds him with his own arm and then sends him flying with his G-Kick, causing him to collapse and explode.
LH binds to the interstitial cells, causing them to secrete testosterone.
* Antagonism ( pharmacology ), when a substance binds to the same site an agonist would bind to without causing activation of the receptor
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