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Without functional RecA protein, the exogenous plasmid DNA is left unaltered by the bacteria.
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Without and functional
Without the dongle, Reason 6 will not open saved files, nor will it dump audio to a sound file, but is otherwise fully functional.
Without this vital piece, Mighty Orbots cannot become fully functional ( a flaw that was exploited once by Umbra, under the machinations of the Shadow Agent called Plasmus, in the episode " The Wishworld ").
Without a stable spine, one aided by proper contraction of the TVA, the nervous system fails to recruit the muscles in the extremities efficiently, and functional movements cannot be properly performed.
Without a functioning supraspinatus, the physician must start abducting the patient's arm and eventually the patient will be able to finish abduction if the deltoid is functional, which is common because the supraspinatus is innervated by the suprascapular nerve from the superior / upper trunk of the brachial plexus.
Without and protein
: Without SDS, different proteins with similar molecular weights would migrate differently due to differences in mass-charge ratio, as each protein has an isoelectric point and molecular weight particular to its primary structure.
Useful in all smoking applications and with any kind of animal protein, it is best used with fish where the flesh of, say, Salmon, forms a pellicle, the surface that will attract more smoke to adhere to it than would be the case if you had not used it: Without a pellicle ; the fish would be inedibly dry from enough smoking to produce a tasty finished product.
Without mRNA essential protein synthesis and hence cell metabolism grind to a halt and the cell dies.
Without the protein, the crystal structure would be too brittle to keep its form and the organic matrix is thought to have a role in deposition of calcium during the mineralization process.
Without and DNA
Without histones, the unwound DNA in chromosomes would be very long ( a length to width ratio of more than 10 million to one in human DNA ).
( Without proofreading error rates are a thousandfold higher ; because many viruses rely on DNA and RNA polymerases that lack proofreading ability, they experience higher mutation rates.
Without telomeres at the end of DNA, this genetic sequence at the end of the chromosome would be deleted.
Without a DNA sample, it is not possible to reconstruct the complete genetic makeup ( genome ) of any individual who died very long ago.
Without the virus, a Protector will weaken and die as his DNA is degraded ; the virus supplies replacement DNA.
Without the DNA methyltransferase ( DNMT ), the replication machinery itself would produce daughter strands that are unmethylated and, over time, would lead to passive demethylation.
Without a known family history of LHON the diagnosis usually requires a neuro-ophthalmological evaluation and blood testing for mitochondrial DNA assessment.
Without and is
Without saying or seeming to say that in portraying the Sartoris and the Compson families Faulkner's chief concern is social criticism, we can say nevertheless that through those families he dramatizes his comment on the planter dynasties as they have existed since the decades before the Civil War.
Without a precise knowledge of Germanic philology, however, it is debatable whether their use was not more often a source of confusion and error than anything else.
Without such forward planning, investment funds are wasted because manufacturing facilities are completed before there is power to operate them or before there is transport to service them ; ;
A true university, like most successful marriages, is a unity of diversities Without forcing all components into a single pattern, the preparation of a master plan is an opportunity to consider interrelation of knowledge at its highest level, which a university -- in contrast to a multiversity -- should stand for.
Without losing the distinctive undertow of Brahmsian rhythm, the pacing is firm and the over-all performance has a tightly knit quality that makes for maximum cumulative effect.
`` Without any officious and improper interference on the subject, the price of labor or the wages of mechanics will be regulated by the demand for the manufactured article and the value of that which is paid for it ; ;
Without such a declaration, Paine concluded, “ he custom of all courts is against us, and will be so, until, by an independence, we take rank with other nations .”
Without this money creation benefit, it is actually more expensive to outsource the IT operations as the outsourcing adds a layer of management and increases overhead.
Chapter 17 of William Bates ' 1920 book Perfect Sight Without Glasses, in which the author argues that observation of the sun is beneficial to those with poor vision, includes a figure of somebody " Focussing the Rays of the Sun Upon the Eye of a Patient by Means of a Burning Glass.
Without a freewheel, coasting is impossible, so when the rear wheel is moving, the cranks are moving.
Without diagnosis and treatment, the infection can be fatal in one to six days ; mortality in untreated cases is approximately 100 %.
Without clear documentation, software can be hard to use — especially if it is very specialized and relatively complex like Photoshop or AutoCAD.
Without protected memory, it is possible that a bug in one program will alter the memory used by another program.
Without salvation from sin ( see below ), a person's separation from God is permanent, causing such a person to enter Hell in the afterlife.
Guided by Arkyn, Corum, Rhalina and companion Jhary-a-Conel cross the planes and encounter the King Without A Country, the last of his people who in turn is seeking the City in the Pyramid.
According to Tibor R. Machan, " Without a market in which allocations can be made in obedience to the law of supply and demand, it is difficult or impossible to funnel resources with respect to actual human preferences and goals.
: Without doubt this is the most important work on general algebra that the Annalen has ever published.
Without experience of meditation, one's knowledge of the world is too limited to fully understand dukkha, as required by the first noble truth, and proceed to enlightenment.
" Without these questions there is no clear fulcrum on which to balance law, politics, and the practice of arbitration — in fact, no common assumptions of all participants — so the ability to formulate the questions are prior to rights balancing.
Without a price mechanism, socialism lacks the means to relate consumer satisfaction to economic activity, it is argued.
The scene in which Lee states that his style was the style of " Fighting Without Fighting " and then lures Parsons into boarding a dinghy is based upon a famous anecdote involving the 16th century samurai Tsukahara Bokuden.
Without this suffix, a countable noun is understood to be singular.
Without and left
Without such uncertainty we are left with a set of dogmas and myths.
Without apostles or prophets left on the earth with the legitimate Priesthood Authority, the true teachings and practices of Christianity were lost.
Without a director, the series was put on hold and Kawamori left to work on other projects.
In 2007, President Chen proposed a policy of Four Wants and One Without, which in substance states that Taiwan wants independence ; Taiwan wants the rectification of its name ; Taiwan wants a new constitution ; Taiwan wants development ; and Taiwanese politics is without the question of left or right, but only the question of unification or independence.
Without the help of Dors, Seldon never would have left it alive.
" Continuing, Krashen wrote, " Without a serious, dedicated and organized campaign to explain and defend bilingual education at the national level, in a very short time we will have nothing left to defend.
Without a local government, residents were unable to make decisions that were instead left to the Town of Islip and Suffolk County.
Without the Sacred to confer an absolute, objective value upon historical events, modern man is left with " a relativistic or nihilistic view of history " and a resulting " spiritual aridity ".
Without being able to appear publicly, the leadership was left to navigate underground.
Without any agents left, the disgraced Leamas is recalled to the Circus in London by Control, chief of the Circus.
Without Richard guiding the audience through the dramatic action, the audience is left to evaluate for itself what is going on.
Without help from his partner, he left many clues and police apprehended him the next day.
Without funding sources for work in the U. S. or Italy, Luria left his home country for Paris, France in 1938.
Without any knowledge of the whereabouts of Duerst and Streiff, the former residents of Glarus left Baltimore searching for the two men who arrived before them and the land chosen for their new home.
Without many words, Oliver asks her to let him divorce her, but clinging to the only thing she has left in her life — her marriage — she refuses.
Without sufficient support and resources to run for elected office again, White left elective politics, but remained active in Republican affairs.
Without hesitation and ignoring attempts to restrain him, Chaplain Watters left the perimeter three times in the face of small arms, automatic weapons, and mortar fire to carry and to assist the injured troopers to safety.
Without explaining himself, Scott left Madelyne and their son to reunite with his lost love and sweetheart.
1987 would see another personnel change within the band as Kim Stone left the band and the bass was taken over by Roberto Vally for the Stories Without Words album.
His tombstone reads: " A day of memory, Sad to recall, Without farewell, He left us all ", and his grave is regularly visited by fans.
Without cavalry support, the Jacobite left also broke, and the Earl of Mar abandoned the area at nightfall.
< td align =" left "> Tres cuentos sin amor ( Three Stories Without Love )</ td >
Without a qualifier, the term ejection fraction basically refers to the blood output of the left ventricle.
Roter received a bachelor's degree in mechanical engineering from the University of Waterloo and left part-way through his master's degree to focus full-time on Engineers Without Borders.
Without having been consecrated and with only the title of bishop-elect and Protector of the Indians, he, accompanied by Andrés de Olmos, left Spain with the first civil officials, auditors ( oidores ), towards the end of August 1528, and reached Mexico on December 6.
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