The method developed by Mullis, however, combined the use of a heat-stable polymerase and thermal cycling, which allowed the rapid and exponential amplification of large quantities of any desired DNA sequence from an extremely complex template.

This method is easier and quicker than the dye primer approach, but may produce more uneven data peaks ( different heights ), due to a template dependent difference in the incorporation of the large dye chain-terminators.

An alternative method of vapor-phase oxidation of SiH4 on the template surface was found to preserve delicate structural features of the microstructure.

A few remnants of Cfront's translation method are still found in today's C ++ compilers ; name mangling was originated by Cfront, as the relatively primitive linkers at the time did not support type information in symbols, and some template instantiation models are derived from Cfront's early efforts.

In software engineering, the template method pattern is a behavioral design pattern that defines the program skeleton of an algorithm in a method, called template method, which defers some steps to subclasses.

In the template method, one or more of the algorithm steps can be overridden by subclasses to allow differing behaviors while ensuring that the overarching algorithm is still followed.

The template method thus manages the larger picture of task semantics, and more refined implementation details of selection and sequence of methods.

The non-abstract methods are completely controlled by the template method, but the abstract methods, implemented in subclasses, provide the pattern's expressive power and degree of freedom.

The template method occurs frequently, at least in its simplest case, where a method calls only one abstract method, with object oriented languages.

The template method may be used to add immediate present value to the software or with a vision to enhancements in the future.

The difference is that the adapter gives the same interface for several operations, while template method does so only for one.

#* The Polymerase Chain Reaction (( PCR ) method of in vitro DNA synthesis uses a DNA template, polymerase, buffers, primers, and nucleotides to multiply the HIV in the blood sample.

If indeed some cancer stem cells use an alternative method of telomere maintenance, it should be noted that they are still killed when the RNA template of telomerase is blocked.

Its innovative method of construction provided the template for the construction in 1890 of the City & South London Railway, the first of London's " Tube " railways.

A reproducible method for creating larger quantities of consistent, high-quality quantum dots involves producing nanoparticles from chemical precursors in the presence of a molecular cluster compound under conditions whereby the integrity of the molecular cluster is maintained and acts as a prefabricated seed template.

One example is that the template method pattern can be reinterpreted for compile time ; so that your main class has a skeleton algorithm, which — at customization points — calls the appropriate functions of some of the policies.

Skeleton programs are utilized in the template method design pattern used in object-oriented programming.

A special template is furnished with the apparatus to enable marking a specimen for a central measuring area and the fabric extensions to the clamps ( see Fig. 2 ).

The Vulcan Presenting Venus with Arms for Aeneas is a template for a tapestry made by this factory.

The order in which the amino acids are added is read through the genetic code from an mRNA template, which is a RNA copy of one of the organism's genes.

In fact, as their synthesis is controlled by a template directed process in most in vivo systems all biopolymers of a type ( say one specific protein ) are all alike: they all contain the similar sequences and numbers of monomers and thus all have the same mass.

He is approached by a woman who explains she is Sarah Tyrell, niece of Eldon Tyrell, heiress to the entire Tyrell Corporation and the human template ( templant ) for the Rachael replicant.

At the same time, the human template for Roy Batty hires Dave Holden, the blade runner attacked by Leon, to help him hunt down the man he believes is the sixth replicant-Deckard.

* Roy Batty: The man which Tyrell used as the template for his combat replicants is in fact a man of considerable instability, suffering from a brain disorder that prevents him from experiencing fear.

There is no set template for what such a vehicle will look like, yet likely features include a large dozer blade or mine ploughs, a large calibre demolition cannon, augers, winches, excavator arms and cranes or lifting booms.

In genetics, complementary DNA ( cDNA ) is DNA synthesized from a messenger RNA ( mRNA ) template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase.

This ' intron-free ' DNA is constructed using ' intron-free ' mRNA as a template.

# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

The double helix is unwound and each strand acts as a template for the next strand.

The notation 5 ' and 3 ' refer to the direction of the DNA template in the chromosome and is used to distinguish between the two untranslated regions ( grey ).

* Harry Beck, a London Underground employee who, in 1931, devised the famous diagrammatic map, which is the template for most rapid transit system maps across the world.

* 1986 – Dragon Quest, the game credited as setting the template for role-playing video games, is released in Japan.

mRNA is transcribed from a DNA template, and carries coding information to the sites of protein synthesis, the ribosomes.

A reaction consisting of the histone octamers and a naked DNA template can be incubated together at a salt concentration of 2 M. By steadily decreasing the salt concentration, the DNA will equilibrate to a position where it is wrapped around the histone octamers, forming nucleosomes.

A Class is a template for creating objects of a particular type, having their own implementations.

Most organisms then process the pre-mRNA ( also known as a primary transcript ) using various forms of Post-transcriptional modification to form the mature mRNA, which is then used as a template for protein synthesis by the ribosome.

The process of synthesizing a protein from an mRNA template is known as translation.

The most successful type of structure prediction, known as homology modeling, relies on the existence of a " template " structure with sequence similarity to the protein being modeled ; structural genomics ' goal is to provide sufficient representation in solved structures to model most of those that remain.

Although producing accurate models remains a challenge when only distantly related template structures are available, it has been suggested that sequence alignment is the bottleneck in this process, as quite accurate models can be produced if a " perfect " sequence alignment is known.

Lee returned the favour by going on to direct their 1999 Edinburgh show, Arctic Boosh, which remains the template of all their live work.

He continues with the fourth form also containing elements of the ideal template / archetype which in this way remains an ever-present and invisible ideal version with which the craftsman compares his work.

While crime has increased to an extent in the area, New Brighton remains a vibrant neighborhood that contains many historic attractions, as well as a place of community that is dedicated to improving and reviving the area .< ref > NYPL Timeline of Staten Island < http :// www. nypl. org / branch / staten / index2. cfm? Trg = 1 & d1 = 962 & template = timeline5 ></ ref >

Wonder Man remains in suspended animation for years, and it is during this period that Ultron, the evil robot creation of Henry Pym, steals the brain patterns recorded by the Avengers for use as a template for the synthezoid Vision.