These cells were thawed at 37-degrees-C for 30 min and were deglycerolized by alternately centrifuging and mixing with descending concentrations of glycerol solutions ( 20, 18, 10, 8, 4 and 2% ).

These have been labeled Regions 1, 2, and 4, respectively, in Fig. 1.

These scholars also maintain that Jesus was the only human ever elected and that individuals must be " in Christ " ( Eph 1: 3 – 4 ) through faith to be part of the elect.

These were differently sized in different countries, for instance, the historical French acre was 4, 221 square metres, whereas in Germany as many variants of " acre " existed as there were German states.

These included a speed of 230. 4 kbit / s, 1000 feet maximum distance, and only 32 nodes per LAN.

These appointments continued in force till the end of David's reign ( 1 Kings 4: 4 ).

These are: ( 1 ) the presence of a living being, human or animal ; ( 2 ) the knowledge that the being is a living being ; ( 3 ) the intent to kill ; ( 4 ) the act of killing by some means ; and ( 5 ) the resulting death.

These results represent another decrease on previous years ( 24. 8 % in 2001 and 26. 4 in 1996 ).

These actions were often bizarre actions that violated the cultural norms of the time ( e. g. Ezekiel 4: 4-8 ).

These were the Yaoundé II Declaration of 4 April 1971 and the Maroua Declaration of 1 June 1975, which were devised to outline maritime boundaries between the two countries following their independence.

These shells can be constructed from pentamers and hexamers by minimizing the number T ( triangulation number ) of nonequivalent locations that subunits occupy, with the T-number adopting the particular integer values 1, 3, 4, 7, 12, 13 ,...( T

These tubes didn't need gas in them to work, so they were evacuated to a lower pressure, around 10 < sup >− 9 </ sup > atm ( 10 < sup >− 4 </ sup > P ).

These reforms were largely directed against John Wycliff, mentioned in the opening session, and condemned in the eighth, 4 May 1415 and Jan Hus, and their followers.

These materials feature isolated carbon centers, often described as " C < sup > 4 −</ sup >", in the methanides or methides ; two-atom units, " C < sub > 2 </ sub >< sup > 2 −</ sup >", in the acetylides ; and three-atom units, " C < sub > 3 </ sub >< sup > 4 −</ sup >", in the sesquicarbides.

These versions allowed 25 modules be to inserted into a single 5-1 / 4 inch section of racking, and allowed the high densities needed to build a computer.

These books make up the Apocrypha section of the Clementine Vulgate: 3 Esdras ( 1 Esdras ); 4 Esdras ( 2 Esdras ); and The Prayer of Manasseh, where they are specifically described as " outside of the series of the canon ".

These results show that the composition of the salt, as anhydrous chlorides on a weight percentage basis, was calcium chloride ( CaCl < sub > 2 </ sub >) 14. 4 %, potassium chloride ( KCl ) 4. 4 %, magnesium chloride ( MgCl < sub > 2 </ sub >) 50. 8 % and sodium chloride ( common salt, NaCl ) 30. 4 %.

These plant communities cover approximately 9. 4 percent of the Earth's surface ( or 30 percent of total land area ), though they once covered much more ( about 50 percent of total land area ), in many different regions and function as habitats for organisms, hydrologic flow modulators, and soil conservers, constituting one of the most important aspects of the biosphere.

These are typical of fandangos and sevillanas, suggesting their origin as non-Gypsy styles, since the 3 / 4 and 4 / 4 measures are not common in ethnic Gypsy music.

These entities can be divided into categories.

These three entities, covers the history of Albania between the late 12th century, until the half of the 14th century, when Albanian Principalities were created through all Albania.

These 34 " county-level " entities correspond to the first level of local administrative unit for EU and Eurostat purposes.

These are lists of incumbents ( individuals holding offices or positions ), including heads of states or of subnational entities.

These entities have observer status with FATF, which does not entitle them to vote, but permits full participation in plenary sessions and working groups.

These sources include entities with legal obligations to submit reports to the FinTRACA when a suspicious activity is detected, as well as reports of cash transactions above a threshold amount specified by regulation.

These laws, contained in sections 5311 through 5332 of Title 31 of the United States Code, require financial institutions, which under the current definition include a broad array of entities, including banks, credit card companies, life insurers, money service businesses and broker-dealers in securities, to report certain transactions to the United States Treasury.

These entities, along with Benin and other nations which do not border Niger, were semi independent elements of French West Africa.

These fundamental ontological categories provide the basis for communication in an age: a horizon of unspoken and seemingly unquestionable background meanings, such as human beings understood unquestioningly as subjects and other entities understood unquestioningly as objects.

These entities are referred to as complexes of occasions of experience.

These functions are increasingly being outsourced to other entities that can perform the activities better or more cost effectively.

These two different states of qi, on the other hand, are embodiments of the abstract entities of yin and yang, two complimentary extremes that constantly play against and with each other and can not exist without the other.

These remarks from Arafat indicated a shift away from one of the PLO's primary aims — the destruction of Israel ( as entailed in the Palestinian National Covenant )– and toward the establishment of two separate entities: an Israeli state within the 1949 armistice lines, and an Arab state in the West Bank and the Gaza Strip.

These servers work within the hierarchical computing environment which treat users, computers, applications and files as distinct but related entities on the network and grant access based on user or group credentials.

" These states of being were " altogether explicable and indefinite " ( like brother and sister ), and it was the fruits of the union of these two entities that created "... the rocks and sands of the earth ; then trees, shrubbery, herbs and grass ; then animals ".

These entities employ nearly 40, 000 employees in the City of SeaTac and generate total local sales of approximately $ 3. 7 billion.

These outlying zones are sometimes known as a commuter belt, and may extend well beyond the urban zone, to other political entities.

These beings are significantly powerful entities known as devas.

These entities have been very useful in facilitating Fultondale's rapid growth in the last few years.

These entities are examples of nationally successful, long-standing job opportunities for residents of Twin Falls.

These four important branches, the Shiwei, Khitan, Murong and Tuoba all arose as discernible entities in the middle of the 3rd century just after the Xianbei Empire had fallen in 235.

These two entities form the legislative and executive branches of the government.

These were two significant economic entities that contributed to the growth of the Borough.

These terrifying entities were armed with enormous knives and are illustrated in grotesque forms, typically as human figures with the heads of animals or combinations of different ferocious beasts.