With this purified conjugate the best staining procedure consisted of treating the sections with 1: 10 dilution of Af for 30 minutes, washing with PBS for 15 minutes, staining with Af for 30 minutes, and washing with PBS for 15 minutes.

With a recreational abalone licence, there is a bag limit of 10 per day, and a total possession limit of 20.

With these elements, hydride atomization enhances detection limits by a factor of 10 to 100 compared to alternative methods.

With a reserve ratio of 10 %, the bank can create 400 million USD in additional loans ( there is a time lag, and the bank has to expect to recover the loaned money back into its books ).

With United Nations Security Council Resolution 1244, the bombing campaign ended on June 10, 1999.

With around 10, 000 living species, they are the most speciose class of tetrapod vertebrates.

With the Mirage III withdrawn, the air defence of Brasília and region is done temporarily by a mix of F-5s from 1st GAv and 1st / 14th GAv until 10 Mirage 2000Cs and two Mirage 2000B bought from French Air Force surplus stocks arrive at Anápolis.

With 20, 000 men ( including a rate of feminization of nearly 10 %) the Army remains the most important component in terms of numbers.

With over 10 million foreign tourists annually, tourism generates revenue in excess of € 7 billion.

" With total world emissions from fossil fuels growing rapidly, the proportion of fossil-fuel emissions absorbed by the oceans since 2000 may have declined by as much as 10 %, indicating that over time the ocean will become " a less efficient sink of manmade carbon.

* 1978 – With the crew investigating a problem with the landing gear, United Airlines Flight 173 runs out of fuel and crashes in Portland, Oregon, killing 10.

With one third of the game ( 9 levels ) distributed as shareware, Doom was played by an estimated 10 million people within two years of its release, popularizing the mode of gameplay and spawning a gaming subculture ; as a sign of its effect on the industry, games from the mid-1990s boom of first-person shooters are often known simply as " Doom clones ".

With 2, 000 congregations and a membership of some 1. 8 million ( or 10. 8 % of the Dutch population, 2009 ), it is the second largest church in the Netherlands after the Roman Catholic Church.

With ADR, actors can average 10 – 12 lines per hour, while rythmo band can facilitate the reading of 35-50 lines per hour.

With such business implications in mind, David Liddle ( General Manager, Xerox Office Systems ) and Metcalfe ( 3Com ) strongly supported a proposal of Fritz Röscheisen ( Siemens Private Networks ) for an alliance in the emerging office communication market, including Siemens ' support for the international standardization of Ethernet ( April 10, 1981 ).

With a $ 10 million dollar investment, Paramount built their own chain of first-run movie theaters after a secret plan to merge with First National failed.

Line No. 2 PC ( inner ring 2 ), serves the same areas as the PC one in reverse. With a bus every hour from Monday to Saturday. Amplitude ( 1 bus ): 6 h beginning 10, 20 end 10 h.

With McMahon unexpectedly eliminated from the contest, Senator John Gorton was elected Liberal leader on 9 January 1968, and was sworn in as Prime Minister on 10 January, replacing McEwen.

With an annual drift of 10 or 11 days, the seasonal relation repeats about every 33 Islamic years.

With average growth rates of 10 % in the 1960s, 5 % in the 1970s, and 4 % in the 1980s, Japan was able to establish and maintain itself as the world's second largest economy from 1968 until 2010, when it was supplanted by the People's Republic of China.

" With the help of record producers Herb Alpert and Lou Adler, Jan and Dean scored a No. 10 hit with " Baby Talk " ( 1959 ), their first song to contain a few of the soon to be famous elements that became associated with surf ( close vocal harmonies, selective use of major and minor chords, falsetto doo-wop singing ) and then scored a series of hits over the next couple of years.

With numbers, Finnish does not use plurals but partitive singular forms: " 10 markkaa " and " 10 penniä " ( the nominative is penni ).

With the support of Prince Louis the French Heir and of King Alexander II of the Scots, they entered London in force on 10 June 1215, with the city showing its sympathy with their cause by opening its gates to them.

With the preparations finished, Young and Duke undocked in the LM Orion from Mattingly in the Command / Service Module Casper 96 hours, 13 minutes, 13 seconds into the mission.

With a scanning speed of 1. 2 m / s, the playing time is 74 minutes, or 650 MB of data on a CD-ROM.

Line No. 1 dessert habitat areas such as: CHATENAY, HORTH, GRANT, COULEE D ’ OR, JARDIN DE ZEPHIR, BOURDA, COLIBRI, et ZEPHIR, and major traffic generators in the Greater Cayenne ( center city ​​ and administrative district schools ). With a bus every 30 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 5 h beginning 45, 20 end 14 h.

Line No. 2 dessert habitat areas such as: RESIDENCE UNIVERSITAIRE, STANILAS, PASTEUR, RESIDENCE DE BADUEL, and major traffic generators in the Greater Cayenne ( downtown and schools ). With a bus every 35 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 h beginning 15, 01 end 20 h.

Line No. 3 serves residential areas such as: MANGUIER, THEMIRE, MANGO, BONHOMME, NOVA PARC, VENDÔME, CHEMIN TARZAN, MONT-LUCAS, and major traffic generators in the Greater Cayenne ( downtown, administrative district and schools ). With a bus every 22 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 4 buses Max ): 5 h beginning 45, end 19 h 56Line 4

dessert habitat areas such as: RENOVATION URBAINE, EAU LISETTE, URANUS, ROSERAIE, MORTIN, ZENITH ( Matoury ), and major traffic generators in the Greater Cayenne ( downtown, industrial park and schools ). With a bus every 40 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 5 h beginning 50, 20 end 13 h.

Line No. 5 serves residential areas such as: LES ALIZES, ANATOLE, BRUTUS, CESAIRE, EAU LISETTE, BONHOMME, CABASSOU, and major traffic generators in the town of Cayenne ( city, district administrative and schools ). With a bus every 30 minutes Monday to Saturday, and a bus every hour on Saturday afternoon. Amplitude ( 2 bus Max ): 6 pm start 00, end 20 h 04.

With increased popularity in the 1970s most soap operas expanded to an hour in length by the end of the decade ( Another World even expanded to ninety minutes for a short time ).

With less than four minutes to play, a frustrated Alan Page was called for a personal foul for a late hit on Griese, and then one play later both Page and Kuechenberg were given offsetting personal fouls after getting in a scuffle with each other.

With less than two minutes remaining in the half, Dallas advanced to the Pittsburgh 32-yard line, after starting from their own 34-yard line.

With just over 4 minutes left in the half, Montana lead the 49ers on another scoring drive.

With about two minutes left in the half, the Dolphins finally managed to get a good drive going on their next possession.

With the score 24-14 in the closing minutes of the first half, Aikman threw 3 straight incompletions, and a short punt by the Cowboys set up Young's 28-yard touchdown completion to Rice with 8 seconds remaining in the first half.

With under 4 minutes left in the game, the Jaguars reached the New England 5-yard line, but Patriots defensive back Willie Clay intercepted a pass in the end zone.

With less than two minutes left in the first half, Warner completed a pass to receiver Ricky Proehl at the Rams 40-yard line, but New England defensive back Antwan Harris forced a fumble while tackling him, which was recovered by Patriots defensive back Terrell Buckley.

With the score tied 5 – 5 and 1: 26 minutes to go in regulation, Lemieux one-timed a pass from Gretzky on a 3 – on – 1 with defenceman Larry Murphy.

With the enemy airfields only 20 minutes away, the bombers would have dropped their bombs and be returning to base before the intercepting fighters could get to altitude.

With a radio operator initiating a call, the process normally takes a few minutes for the ALE to pick an HF frequency that is optimum for both sides of the communication link.

With the opening of the Great Belt Link, the journey is now between 10 and 15 minutes.

With the score at 1 – 1 after 90 minutes, Hiddink and Russia went on to win the match 3 – 1.