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For the albumin method, equal volumes of 30% bovine albumin, sample and 2% cells suspended in saline were allowed to stand at room temperature for 1 hr and then were centrifuged at 1000 rpm for 1 Aj.
The saline and albumin tests were performed as described for the ABO samples except that the mixture was incubated for 1 hr at 37-degrees-C before centrifugation.
Blood samples were allowed to clot at room temperature for 3 hr, centrifuged and the serum was removed.
The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.
Five milliamperes/cell were applied for 18 hr, after which the strips were stained with bromphenol blue and densitometry was carried out using a Spinco Analytrol.
At the end of the run, the strips in the third and sixth positions in each chamber were dried, stained for 1 hr, washed and dried, while the other strips were maintained in a horizontal position at 1-degree-C.
Fractions from the column which were to be subjected to analytical ultracentrifugation were concentrated by negative pressure dialysis and dialyzed for 16 hr in the cold against at least 500 volumes of phosphate-buffered saline, pH 7.2, ionic strength 0.154.
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606 and .
353 U. S., at 606.
Charlton also held the club record for most league appearances ( 606 ) until this too was overtaken by Giggs on 6 March 2011.
In Forbes national university ranking, Bob Jones was unranked in 2011, # 606 in 2010, and # 279 in 2009.
Military forces in non-Soviet Berlin sectors totaled only 8, 973 Americans, 7, 606 British and 6, 100 French.
The largest failure for the Kampfgeschwader, however, was saddling them with an aircraft meant to be as big and as apparently capable as any Allied four-engined " heavy bomber " – the perpetually-troubled Heinkel He 177 – which had been condemned to being designed to carry out moderate angle dive bombing missions from the day that the RLM accepted it for production in November 1937, making it overweight from the start, and mandating design features that led its " welded-together engines ", a pair of cumbersome Daimler-Benz DB 606 " power systems ", to perpetually catching fire in flight during operational missons over both the Eastern Front and the United Kingdom.
On the death of Pope Sabinian in February 606, Boniface was elected his successor, although his return from Constantinople to Rome was delayed by almost a year.
During the reign of Harsha ( r. 606 – 647 ) in North India, Indian envoys in Tang China taught methods of cultivating sugarcane after Emperor Taizong of Tang ( r. 626 – 649 ) made his interest in sugar known.
Receiver Paul Warfield once again provided the run-based Dolphins with an effective deep threat option, catching 29 passes for 606 yards, an average of 20. 9 yards per catch.
Multi-talented veteran wide receiver Don Beebe was also a constant breakaway threat, catching 39 passes for 606 yards and adding another 403 yards, and a touchdown returning kickoffs.
* 606: Boniface elected papal successor on the death of Pope Sabinian.
* Jianzhi Sengcan, ( d. 606 ), third Chán Buddhist Patriarch
* 606 BC: King Ding of Zhou becomes King of the Zhou Dynasty of China.
Year 606 ( DCVI ) was a common year starting on Saturday ( link will display the full calendar ) of the Julian calendar.
The denomination 606 for this year has been used since the early medieval period, when the Anno Domini calendar era became the prevalent method in Europe for naming years.
am: 606...
lt: 606 m.
The median income for a household in the city was $ 36, 606, and the median income for a family was $ 45, 410.
82, No. 3, May / June 1974, pp. 598 – 606.

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