After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

" After much bargaining, the famine-stricken citizens agreed to pay a ransom of 5, 000 pounds of gold, 30, 000 pounds of silver, 4, 000 silken tunics, 3, 000 hides dyed scarlet, and 3, 000 pounds of pepper.

After his brother's death on 30 September / November 1204, Andrew took over the government of the kingdom as his nephew's tutor and he also seized the money his brother had deposited on behalf of the child Ladislaus.

After three years of searching, on December 30, 1907, Spalding received a letter that ( erroneously ) declared baseball to be the invention of Abner Doubleday.

After a recent increase in profits by 60 %, the company refused to raise worker's pay by more than 30 %.

After Mark Antony's death, Cleopatra eluded the vigilance of her guard Epaphroditus and committed suicide, on 12 August 30 BC.

After premiering on September 30, 1982, it was nearly canceled during its first season when it ranked last in ratings for its premiere ( 74th out of 77 shows ).

After the trade, however, Colavito hit over 30 home runs four times and made three All-Star teams for Detroit and Kansas City before returning to Cleveland in.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After losing each of the first two games by two runs, the Rockies opened up a 5 – 2 lead against Matt Cain in the finale of the series on August 30.

After some reverses, UN and Congolese government forces succeeded in recapturing the breakaway provinces of South Kasai on 30 December 1961, and Katanga on 15 January 1963.

After several script rewrites and changes, including a second title change to Friends Like Us, the series was finally named Friends and premiered on NBC's coveted Thursday 8: 30 pm timeslot.

After dating for nine months, the couple were married on October 30, 1943.

After a heated debate, the President of the Volkskammer, Sabine Bergmann-Pohl, announced the results at 2: 30 am on 23 August:

After almost 30 years of its release, Gaynor continues to ride the success of " I Will Survive ", touring the country and the world over and performing her signature song on dozens of TV shows.

After U. S. President Barack Obama announced the deployment of another 30, 000 soldiers in 2010 for a period of two years, Der Spiegel published images of the US soldiers who killed unarmed Afghan civilians.

After a medical examination three days later, Richard was told to rest his arm and he collapsed during a July 30 workout.

After Napoleon imposed the Convention of Artlenburg ( Convention of the Elbe ) on July 5, 1803, about 30, 000 French soldiers occupied Hanover.

After repeated negotiations between the Basel Council and Bohemia, a Bohemian – Moravian state assembly in Prague accepted the " Compacta " of Prague on 30 November 1433.

After about 30 years, the Japanese developed scale tuning and semitone harmonicas to be able to perform Japanese folk songs.

After the vote of the Partition Plan for Palestine on 30 November 1947, civil war broke out in Palestine.

* 1813 – War of 1812: After learning of American plans for a surprise attack on Beaver Dams in Ontario, Laura Secord sets out on a 30 kilometer journey on foot to warn Lieutenant James FitzGibbon.

After their joint suicide in 30 BCE, Octavian assumed control of the Roman Empire and granted Herod free rein over Jericho, as part of the new Herodian domain.

After being closed for seven months, the Garden of the Gods Club will have its gala summer opening Saturday, June 3.

After the capture of the city in June, 1098, and the subsequent siege led by Kerbogha, Adhemar organized a procession through the streets, and had the gates locked so that the Crusaders, many of whom had begun to panic, would be unable to desert the city.

After thirty days of working with Wilson, Smith drank his last drink on June 10, 1935, the date marked by AA for its anniversaries.

After March it began to pass northwards, a motion quite apparent by the middle of April ; in June it passed at the same distance from the zenith as it did in December ; and in September it passed through its most northerly position, the extreme range from north to south, i. e. the angle between the March and September positions, being 40 ″.

After nearly 15 years together, Bartók divorced Márta in June 1923.

After performing for Queen Elizabeth II at a command performance in 1979, Haley made his final performances in South Africa in May and June 1980.

After the Marshall Plan, the introduction of a new currency to Western Germany to replace the debased Reichsmark and massive electoral losses for communist parties in 1946, in June 1948, the Soviet Union cut off surface road access to Berlin.

After disagreements between Yugoslavian leader Josip Broz Tito and the Soviet Union regarding Greece and the People's Republic of Albania, a Tito-Stalin split occurred, followed by Yugoslavia being expelled from the Cominform in June 1948 and a brief failed Soviet putsch in Belgrade.

After two years of negotiations, the United States and Chile signed an agreement in June 2003 that will lead to completely duty-free bilateral trade within 12 years.

After many buffetings and, finally, after the catastrophic results of the June European Parliament election, 2004 Špidla resigned after a revolt in his own party and the government was reshuffled on the same basis.

After a rough start, which included a brawl between Michael Barrett and Carlos Zambrano, the Cubs overcame the Milwaukee Brewers, who had led the division for most of the season, with winning streaks in June and July, coupled with a pair of dramatic, late-inning wins against the Reds, and ultimately clinched the NL Central with a record of 85 – 77.

After several on-again / off-again talks with Atari in May and June 1984, Tramiel had secured his funding and bought Atari's Consumer Division ( which included the console and home computer departments ) in July.

After the Japanese attack on Pearl Harbor, Eisenhower was assigned to the General Staff in Washington, where he served until June 1942 with responsibility for creating the major war plans to defeat Japan and Germany.

After an urgent call for help from the Legation, Seymour set out on 10 June with 2000 troops to attempt to break through to Peking.

After negotiations involving both Edward and Ottbuono Gloucester relented, and by June a settlement was reached.

After the Bolshevik Revolution of 1917, at the age of 22, Vertov began editing for Kino-Nedelya (, the Moscow Cinema Committee's weekly film series, and the first newsreel series in Russia ), which first came out in June 1918.

After her child was born, she had breast implants and appeared in a June 1989 Playboy pictorial, but her career remained in the doldrums.

After tension between Russia and Britain in Europe ended with the June 1878 Congress of Berlin, Russia turned its attention to Central Asia.

After more light rain in June, the lake finally began to dry enough for an attempt to be made.

After 20 years with Milan, Baresi announced his retirement as a player on 24 June 1997.

After the Allied liberation of Rome on June 4, 1944, Fellini and Enrico De Seta opened the Funny Face Shop where they survived the postwar recession drawing caricatures of American soldiers.

After completing the 10-month course, he was commissioned as an ensign in the U. S. Naval Reserve at Corpus Christi, Texas on June 9, 1943, just three days before his 19th birthday, which made him the youngest naval aviator to that date.

* 1469 3 June – After the death of Alfonso de Castilla and the 1st Duke of Medina Sidonia, his son and heir Enrique de Guzman, 2nd Duke of Medina Sidonia changed side and in reward, saw the status of Gibraltar, as part of the domains of the Duke, confirmed by the Queen Isabella I of Castile.

After Heydrich's death in June 1942, and as the war progressed, Müller's power and the independence grew substantially.

After the murder of Foreign Minister Walther Rathenau on 24 June, Himmler's political views veered towards the radical right, and he took part in demonstrations against the Treaty of Versailles.