After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.

After drying the sections under the fan, fixing in acetone, and drying at 37-degrees as in the indirect method, the sections were treated with conjugated Af or Af ( undiluted unless mentioned otherwise ) for 5 - 30 minutes.

" After much bargaining, the famine-stricken citizens agreed to pay a ransom of 5, 000 pounds of gold, 30, 000 pounds of silver, 4, 000 silken tunics, 3, 000 hides dyed scarlet, and 3, 000 pounds of pepper.

After his brother's death on 30 September / November 1204, Andrew took over the government of the kingdom as his nephew's tutor and he also seized the money his brother had deposited on behalf of the child Ladislaus.

After three years of searching, on December 30, 1907, Spalding received a letter that ( erroneously ) declared baseball to be the invention of Abner Doubleday.

After a recent increase in profits by 60 %, the company refused to raise worker's pay by more than 30 %.

After Mark Antony's death, Cleopatra eluded the vigilance of her guard Epaphroditus and committed suicide, on 12 August 30 BC.

After premiering on September 30, 1982, it was nearly canceled during its first season when it ranked last in ratings for its premiere ( 74th out of 77 shows ).

After the trade, however, Colavito hit over 30 home runs four times and made three All-Star teams for Detroit and Kansas City before returning to Cleveland in.

After starting the season 15 – 35, though, the team had some success later in the year, going a respectable 30 – 28 in August and September as the youngsters became more experienced.

After losing each of the first two games by two runs, the Rockies opened up a 5 – 2 lead against Matt Cain in the finale of the series on August 30.

After some reverses, UN and Congolese government forces succeeded in recapturing the breakaway provinces of South Kasai on 30 December 1961, and Katanga on 15 January 1963.

After several script rewrites and changes, including a second title change to Friends Like Us, the series was finally named Friends and premiered on NBC's coveted Thursday 8: 30 pm timeslot.

After dating for nine months, the couple were married on October 30, 1943.

After a heated debate, the President of the Volkskammer, Sabine Bergmann-Pohl, announced the results at 2: 30 am on 23 August:

After almost 30 years of its release, Gaynor continues to ride the success of " I Will Survive ", touring the country and the world over and performing her signature song on dozens of TV shows.

After U. S. President Barack Obama announced the deployment of another 30, 000 soldiers in 2010 for a period of two years, Der Spiegel published images of the US soldiers who killed unarmed Afghan civilians.

After a medical examination three days later, Richard was told to rest his arm and he collapsed during a July 30 workout.

After Napoleon imposed the Convention of Artlenburg ( Convention of the Elbe ) on July 5, 1803, about 30, 000 French soldiers occupied Hanover.

After repeated negotiations between the Basel Council and Bohemia, a Bohemian – Moravian state assembly in Prague accepted the " Compacta " of Prague on 30 November 1433.

After about 30 years, the Japanese developed scale tuning and semitone harmonicas to be able to perform Japanese folk songs.

After the vote of the Partition Plan for Palestine on 30 November 1947, civil war broke out in Palestine.

* 1813 – War of 1812: After learning of American plans for a surprise attack on Beaver Dams in Ontario, Laura Secord sets out on a 30 kilometer journey on foot to warn Lieutenant James FitzGibbon.

After their joint suicide in 30 BCE, Octavian assumed control of the Roman Empire and granted Herod free rein over Jericho, as part of the new Herodian domain.

After the longest reign since Augustus ( surpassing Tiberius by a couple of months ), Antoninus died of fever at Lorium in Etruria, about twelve miles ( 19 km ) from Rome, on 7 March 161, giving the keynote to his life in the last word that he uttered when the tribune of the night-watch came to ask the password —" aequanimitas " ( equanimity ).

After the completion of the Plovdiv-Dimitrovgrad high-speed line on 1 July 2012 the top operating speed was raised to 200 km / h and the national top speed record of 197 km / h set between Iskar and Elin Pelin with a leased Siemens Taurus electric locomotive is soon expected to be broken.

After the war he was ill for many months, recovering at the family's ancestral home in Duke's Denver, a fictional setting — as is the Duchy of Denver — about 15 miles ( 24 km ) beyond the real Denver in Norfolk, on the A10 near Downham Market.

After walking a further 20 miles, the three remaining men made their final camp on 19 March, short of One Ton Depot, but 24 miles ( 38 km ) beyond the original intended location of the depot.

After test failures with the first three flight tests because of guidance and sensor problems, the fourth and final test on June 10, 1984 was successful, intercepting the Minuteman RV with a closing speed of about 6. 1 km / s at an altitude of more than 160 km.

After docking with an Agena target vehicle, the astronauts then set a world altitude record of 474 miles ( 763 km ).

* July 24 – Sack of Cuenca ( Third Carlist War ): After Carlist forces successfully defended Estella, Don Alfonso de Bourbon, brother of the Don Carlos VII, led 14, 000 Catalan Carlists south to attack Cuenca 136 km from Madrid, held by Republicans under Don Hilario Lozano.

After being placed on the list of endangered World Heritage Sites in 2006, the city had its status as world heritage site formally removed in June 2009, for the wilful breach of the UNESCO World Heritage Convention, due to the construction of a highway bridge across the valley within 2 km of the historic centre.

After a 101-day, 4, 300 nautical mile ( 4, 948 miles or 7, 964 km ) journey across the Pacific Ocean, Kon-Tiki smashed into the reef at Raroia in the Tuamotu Islands on August 7, 1947.

After 293 km the Werra joins the river Fulda in the town of Hann.

After boundary changes between 1873 and 1952, the land area of Brighton increased from < span style =" white-space: nowrap "> 1, 640 acres ( 7 km < sup > 2 </ sup >)</ span > in 1854 to < span style =" white-space: nowrap "> 14, 347 acres ( 58 km < sup > 2 </ sup >)</ span > in 1952.

After managing to cover 700 km, predominantly by rowboat, escaping from Singapore, spent 3 years working on the Burma railway.

After the skirmish, President Johnson ordered the Maddox and Turner Joy to stage daylight runs into North Vietnamese waters, testing the twelve-mile ( 19 km ) limit and North Vietnamese resolve.

After descending a narrow spiral stone stairwell of 19 metres to the darkness and silence broken only by the gurgling of a hidden aqueduct channelling local springs away from the area, and after passing through a long ( about 1. 5 km ) and twisting hallway of mortared stone, visitors find themselves before a sculpture that existed from a time before this part of the mines became an ossuary, a model of France's Port-Mahon fortress created by a former Quarry Inspector.

After a few hours at about 300 km altitude, the lander was reoriented for atmospheric entry.

After several hours, Chadwick was forced to give up with stomach pains and vomiting, while 16-year-old Bell completed the swim, the first person ever to swim the thirty-two-mile ( 52 km ) distance when she arrived in Toronto the next day.

After a few hours, at about 300 km attitude, the lander was reoriented for entry.

After about six hours of heavy fighting the attack was stopped about a mile ( 1. 6 km ) short of their objective.

After entering the atmosphere at approximately 6 km / s, the descent system on the module malfunctioned, possibly because the angle of entry was too steep.

After a moderate level, 3. 5 km return walk through an open valley, visitors can enjoy the usually un-crowded plunge pool beneath Tjaynera Falls.

After the 1903 flood damaged and endangered much of the town, it was relocated about one mile ( 1. 6 km ) north to its present location out of “ the bottoms ” ( as they are still known today ) near the river.

After it served for several years as the territorial capital, the territory built a new capital, named Washington, six miles ( 10 km ) to the east and also in Adams County.