After treatment with hot alkali solution ( e. g., 5 – 8 % potassium hydroxide ), the cellulose is removed from the carrageenan by centrifugation and filtration.

After harvesting by liposuction, the breast-filler fat was obtained by low G-force syringe centrifugation of the harvested fat to separate it, by density, from the crystalloid component.

After removing the precipitate by filtration or centrifugation, the desired protein can be precipitated by altering the salt concentration to the level at which the desired protein becomes insoluble.

After centrifugation, the agarose beads will form a very loose fluffy pellet at the bottom of the tube.

After introduction into the vacuum system, the sample is reduced to cryogenic temperatures ( typically 20-100 K ) and manipulated such that the needle's point is aimed towards an ion detector.

After a certain period, typically a month, the columns in each journal are each totaled to give a summary for the period.

After the point is established, a seven is typically called by simply " 7 out " or " 7 out 7 ".

After recovery from fugue, previous memories usually return intact, but there is typically amnesia for the fugue episode.

After they lay their eggs, females will typically incubate them until they hatch.

After that, Postmodernism was applied to a whole host of movements, many in art, music, and literature, that reacted against tendencies in the imperialist phase of capitalism called " modernism ," and are typically marked by revival of historical elements and techniques.

After the active portion of a seizure, there is typically a period referred to as postictal before a normal level of consciousness returns.

After a period of sitting quietly, the members are typically asked to stand and relax by a ' helper ' ( see below ), who then announces the start of the exercise.

# After hybridization, excess probe is washed from the membrane ( typically using SSC buffer ), and the pattern of hybridization is visualized on X-ray film by autoradiography in the case of a radioactive or fluorescent probe, or by development of color on the membrane if a chromogenic detection method is used.

After introductory life science courses are complete, students typically enroll in labs and apply toxicology principles to research and other studies.

After becoming pregnant, a female hippopotamus will typically not begin ovulation again for 17 months.

After entering the grid system via the plasma sheath the ions are accelerated due to the potential difference between the first and second grid ( named screen and accelerator grid ) to the final ion energy of typically 1-2 keV, thereby generating the thrust.

After an individual ascends the throne, he or she typically continues to reign until death, being unable to unilaterally abdicate per the tenets of constitutional monarchy.

After several hours the temperature is reduced in a controlled and gradual manner, resulting in the formation of flawless columnar crystals that are typically about 5 cm long by 2. 5 cm wide, although they may be grown much larger.

After its cage has been cleaned, a guinea pig will typically urinate and drag the lower body across the floor of the cage to mark its territory.

After this early period Chinese art, like Chinese history, is typically classified by the succession of ruling dynasties of Chinese emperors, most of which lasted several hundred years.

After kidnapping the victim, the kidnappers typically make some sort of demand of the government of the hostage's nation and give a time limit for the demand to be carried out, often 72 hours.

After completing their sketches, the artists typically consulted other paintings, of average size, to add further detail.

After the required number of bit periods for the character length ( 5 to 8 bits, typically ) have elapsed, the contents of the shift register is made available ( in parallel fashion ) to the receiving system.

After the bonds are paid off the road typically reverts to the government agency that authorized the road and owns the land it was built on.

After taking caffè, smokers are usually allowed to light their cigarettes ( the service typically includes a porcelain ashtray ).

After one section is complete it is typically pushed forward over rollers on the bridgehead, and another section built behind it.

After a set amount of time ( usually a few hours, though this depends on the voltage applied across the gel ; protein migration occurs more quickly at higher voltages, but these results are typically less accurate than at those at lower voltages ) the proteins will have differentially migrated based on their size ; smaller proteins will have traveled farther down the gel, while larger ones will have remained closer to the point of origin.

After the barn, this is typically historically the second oldest building on the farm.

After the band calls it a night, the younger adults typically find themselves at one of the many late-night house parties hosted throughout town.

After I paid Monsieur Prieur for Dandy, I brought him home, but he was ill at ease and ran away the same night.

After that, it requires several minutes of concentrated work, including six separate and deliberate actions by a minimum of three men sitting at three separate stations in a bomber, each with another man beside him to help, for an armed bomb to be released.

After the collapse of that desperate and ill-fated campaign the character of the king degenerated for a time into a futility that was not merely pitiable but often ridiculous.

After casting about for a way of describing this spirit, we decided that it would be better to use Mr. Lyford's introduction as an illustration.

( After graduation from the University of Chicago, Paula taught for two years in the normal school at Valley City, North Dakota, then two years at Princeton ( Illinois ) Township High School.

After we got a script and the spots for the songs were blocked out, we'd get together for an hour or so every day.

After allowing for group exposures, it is apparent that other factors must be considered if we are to comprehend fanaticism.

After all, Woodruff owned a competent printing plant and was the logical man for the job.

After Quiney was elected bailiff in September, 1601, without Greville's approval, Greene wrote him that Coke had promised to be of counsel for Stratford and had advised `` that the office of bayly may be exercised as it is taken upon you, ( Sr. Edwardes his consent not beinge hadd to the swearinge of you ) ''.

After a dinner party for which she had come down to New York, Mrs. Lewis and Casanova arrived to see them off, and Elinor Wylie made tart observations that indicated that Lewis had been less discreet than he had promised to be about the real nature of their separation.

After that they had sat for five minutes without saying a word.

After the recommendation of the Department is forwarded to the appeal board, that is the appropriate place for a registrant to lodge his denial.

After the Juniors were welcomed and congratulated for qualifying for the Finals of the Junior Class, Mrs. William H. Long, Jr. was introduced as the first speaker.

-- After 52 rainless days, moisture finally came to Del Mar, resulting in but one workout during the week for most of the horses, and leaving us with less than half our total average rainfall during the season.

After notching it for the keelson, chines and battens, the half-inch plywood transom is secured to it with glue and the same type nails.

After selecting a sheet and inspecting it for flaws ( even the best sometimes has foreign ' nubbins ' on its surface ), I sponge it thoroughly on both sides with clean, cold water.

After it has been seen, have the child start on a mat on hands and knees ( a thin, inexpensive mat is quite sufficient for anything that does not require falling ).

After the first month, rates are considerably less, averaging only about $60 a month for most 4- and 5-passenger models.

After Gagarin became the Greatest Man in the World, for a nation that does not believe in the cult of personality or in careerism, Moreland wrote me a letter in which he said: `` I am not interested in how long a bee can live in a vacuum, or how far it can fly.

After it has reached terminal velocity, the time for the tape to travel a known distance is recorded.

After another two weeks, the first young emerge, four to eight small daughters that begin to play the role of worker bees, collecting pollen and nectar in the field and caring for the new young generation while the queen retires to a life of egg laying.

After blotting out most of the saline around the sections, a drop of Af was layered over each of the sections, allowed to react for 30 minutes, and then washed with PBS for 15 - 30 minutes.