In the case of HPLC the mobile phase consists of a non-polar solvent ( s ) such as hexane in normal phase or polar solvents in reverse phase chromotagraphy and the sample being separated.

Hydrophobic molecules tend to be non-polar and, thus, prefer other neutral molecules and non-polar solvents.

On the other hand, non-polar solutes dissolve better in non-polar solvents.

As a rule of thumb, polar solvents dissolve polar compounds best and non-polar solvents dissolve non-polar compounds best: " like dissolves like ".

Strongly polar compounds like sugars ( e. g., sucrose ) or ionic compounds, like inorganic salts ( e. g., table salt ) dissolve only in very polar solvents like water, while strongly non-polar compounds like oils or waxes dissolve only in very non-polar organic solvents like hexane.

The solvents are grouped into non-polar, polar aprotic, and polar protic solvents and ordered by increasing polarity.

They are widely used as cheap, relatively safe, largely unreactive, and easily evaporated non-polar solvents.

NP-HPLC uses a non-polar, non-aqueous mobile phase, and works effectively for separating analytes readily soluble in non-polar solvents.

It is less soluble in non-polar solvents such as benzene, toluene and xylene, and practically insoluble in petroleum ether.

Other common non-polar solvents include benzene, turpentine and other terpenes ( which constitute essential oils of many plants with strong scents ), most ethers, chloroform and dichloromethane, hydrocarbon fuels, and diacetone alcohol, among many others.

Lipophilicity, ( Gr. fat-liking ), refers to the ability of a chemical compound to dissolve in fats, oils, lipids, and non-polar solvents such as hexane or toluene.

Different enzymes have different specificity for their substrate ; trypsin for example cleaves the peptide bond after a positively charge residue ( arginine and lysine ), chymotrypsin cleaves the bond after an aromatic residue ( phenylalanine, tyrosine, and tryptophan ), elastase cleaves the bond after a small non-polar residue such as alanine or glycine.

Raoult's Law is applicable only to non-electrolytes ( uncharged species ); it is most appropriate for non-polar molecules with only weak intermolecular attractions ( such as London forces ).

In general, an analyte with a larger hydrophobic surface area ( C-H, C-C, and generally non-polar atomic bonds, such as S-S and others ) is retained longer because it is non-interacting with the water structure.

* Carbon-based compounds – Are typically hydrophobic and non-polar, including materials such as activated carbon and graphite.

Properties of a good mounting medium include having a refractive index close to that of glass ( 1. 518 ), non-reactivity with the specimen, stability over time without crystallizing, darkening, or changing refractive index, solubility in the medium the specimen was prepared in ( either aqueous or non-polar, such as xylene or toluene ), and not causing the specimen stain to fade or leach.

The non-polar ice caps, such as found on mountain tops, were traditionally ignored as serious places to drill ice cores because it was generally believed the ice would not be more than a few thousand years old, however since the 1970s ice has been found that is older, with clear chronological dating and climate signals going as far back as the beginning of the most recent ice age.

A permanent marker can also be removed by drawing over it with a dry erase marker on non-porous surfaces such as a whiteboard as dry erase markers also contain a non-polar solvent.

β-Carotene is a non-polar compound, so it is separated with a non-polar solvent such as hexane.

First, an organic solvent, such as hexane, is added to the plant material to help extract the non-polar compounds.

A separatory funnel, also known as separation funnel, separating funnel, or colloquially sep funnel, is a piece of laboratory glassware used in liquid-liquid extractions to separate ( partition ) the components of a mixture into two immiscible solvent phases of different densities Typically, one of the phases will be aqueous, and the other a non-polar lipophilic organic solvent such as ether, MTBE, dichloromethane, chloroform, or ethyl acetate.

Commonly used solvents are polar aprotic solvents such as THF, DMSO, Acetonitrile, DMF as well as in non-polar aprotic solvents such as toluene.

Van der Waals gases such as methane, however, have weak cohesion due only to Van der Waals forces that operate by induced polarity in non-polar molecules.

In general, aqueous ( or water-based ) solutions, being polar, are immiscible with non-polar organic solvents ( chloroform, toluene, hexane etc.

A non-polar diluent favours the formation of uncharged non-polar metal complexes.

In the classical branches of continuum mechanics the development of the theory of stresses is based on non-polar materials.

This paper is made of cellulose, a polar substance, and the compounds within the mixture travel farther if they are non-polar.

The bond in a homonuclear diatomic molecule is non-polar.

This ability comes from the fact that bond character between carbon atoms is entirely non-polar, in that the distribution of electrons between the two elements is somewhat even due to the same electronegativity values of the elements (~ 0. 30 ), and does not result in the formation of an electrophile.

Metallic bonding is mostly non-polar, because even in alloys there is little difference among the electronegativities of the atoms participating in the bonding interaction ( and, in pure elemental metals, none at all ).

For the non-polar, but piezoelectric crystals, on the other hand, a polarization P different from zero is only elicited by applying a mechanical load.

The Pamirs in particular are heavily glaciated, and Tajikistan is home to the largest non-polar glacier in the world, the Fedchenko Glacier.

This is used to convert polarised components to non-polar use.

# Dispersion ( usually named after Fritz London ), which is the attractive interaction between any pair of molecules, including non-polar atoms, arising from the interactions of instantaneous multipoles.

The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid.

Heptane ( and its many isomers ) is widely applied in laboratories as a totally non-polar solvent.

The binding of the analyte to the stationary phase is proportional to the contact surface area around the non-polar segment of the analyte molecule upon association with the ligand on the stationary phase.

It is non-polar and cheap.

Activated carbon is used for adsorption of organic substances and non-polar adsorbates and it is also usually used for waste gas ( and waste water ) treatment.

Free in solution, long-range, non-polar interactions can only occur at a high cost in entropy.

When the GroEL non-polar surfaces are removed, the chance that any given non-polar group will encounter a non-polar intramolecular site are much greater than in bulk solution.