In NHEJ, DNA Ligase IV, a specialized DNA ligase that forms a complex with the cofactor XRCC4, directly joins the two ends.

Artemis is a nuclease and is thought to be required only for repair of DSBs with damaged ends, whereas DNA Ligase IV and XLF are required for all NHEJ events.

DNA Ligase then acts to join the two ends together.

* Ligase chain reaction, a method for DNA amplification similar to the polymerase chain reaction

Most experiments use T4 DNA Ligase ( isolated from bacteriophage T4 ), which is most active at 25 ° C.

Ligase works to fill these nicks in, thus completing the newly replicated DNA molecule.

DNA Ligase then joins the various fragments together into a continuous strand of DNA.

Further research implicates the supplementary role of Proliferating cell nuclear antigen ( PCNA ) to DNA Ligase I ’ s function of joining Okazaki fragments.

When the PCNA binding site on DNA Ligase I is inactive, DNA Ligase I ’ s ability to connect Okazaki fragments was severely impaired.

Then, DNA Ligase I binds to the PCNA, which is clamped to the nicks of the lagging strand, and catalyzes the formation of phosphodiester bonds.

* Highwire ( protein ), an E3 Ubiquitin Ligase frequently studied in Drosophila.

`` And next year we will do -- also a Ford commission -- a piano concerto by Elliott Carter, with Jacob Lateiner as soloist.

One day the dogs of Ireland will do that too and perhaps also the pigs ''.

Whether any of us remain in it long will depend on what happens as a result of the technological and economic revolutions now going on in the countries of Asia and Africa, and also of course on how long the cold war remains cold.

Piepsam's fatal rage arises not only because he cannot stop the cyclist, but also because God will not stop him ; ;

Today the private detective will also investigate insurance claims or handle divorce cases, but his primary function remains what it has always been, to assist those who have money in their unending struggle with those who have not.

But to go from here to the belief that those more sensitive to metaphor and language will also be more sensitive to personal differences is too great an inferential leap.

Very likely it will also include a recognition that the work we are reading reflects or `` belongs to '' some way of thought labelled as a `` school '' or an `` -ism '', i.e. a complex or `` syndrome '' of ideas occurring together with sufficient prominence to warrant identification.

We may further grant to those of her ( Poetry's ) defenders who are lovers of poetry and yet not poets, the permission to speak in prose on her behalf: let them show not only that she is pleasant but also useful to States and to human life, and we will listen in a kindly spirit ; ;

And we can add that Krutch's interpretation of purgation is also one answer to Plato's fear that poetry will encourage our passions.

Interestingly enough, the order transmitted to Morgan through Alexander Hamilton also informed him that `` A party of Indians will join the party to be sent from your command at Whitemarsh, and act with them ''.

A more complete list would also include Bradbury's `` The Pedestrian '' ( 1951 ), Philip K. Dick's Solar Lottery ( 1955 ), David Karp's One ( 1953 ), Wilson Tucker's The Long Loud Silence ( 1952 ), Jack Vance's To Live Forever ( 1956 ), Gore Vidal's Messiah ( 1954 ), and Bernard Wolfe's Limbo ( 1952 ), as well as the three perhaps most outstanding dystopias, Frederik Pohl and C. M. Kornbluth's The Space Merchants ( 1953 ), Kurt Vonnegut's Player Piano ( 1952 ), and John Wyndham's Re-Birth ( 1953 ), works which we will later examine in detail.

But I will also remind them that I have always been inclined to skepticism, to a kind of Laodicean lack of commitment so far as public affairs are concerned ; ;

And it is also a fact of life that there will always ( be youngish half-educated people around, who will be dazzled by the glitter of what looks like a literary movement.

Remembering the step-by-step fate of Danzig and the West German misgivings about `` salami '' tactics, it is to be hoped that the dispatch of General Clay to West Berlin as President Kennedy's representative will mark a stiffening of response not only to future indignities and aggressions but also to some that have passed.

it will also correct some injustices.

`` For if ye forgive men their trespasses, your heavenly Father will also forgive you: but if ye forgive not men their trespasses, neither will your Father forgive -- your trespasses ''.

And the pitching will also have trouble doing better.

It should also make him desire to participate actively in civic, school and religious life of the community so that that phase of Newark will live up to the challenge presented by this exhibit.

We now have to think not only of our national security but also of the future generations who will suffer from any tests we might undertake.

I will also be underpaid.

And so, let us remember on this day not only to thank the Almighty Who gave hope and courage to the Pilgrims, but also to place our trust in Him that He will continue to protect us in the future as He has in the past.

This apparatus will also be used to measure transition probabilities of a large number of other elements.

The basement or belowground shelters also will serve for tornado or hurricane protection.

How will we work it out ''??

`` You will stay here thirty minutes after the others go home this afternoon and work your problems ''.

Those familiar with his work will remember that he placed the incipience of the democratic faith at around 1850.

Malraux pretends, perhaps with a trifle too self-conscious a modesty, that his fragmentary work will accordingly `` appeal only to the curiosity of bibliophiles '' and `` to connoisseurs of what might have been ''.

Perhaps tracing some of these more important symbols through the body of his work will show that Patchen's new poetry is well thought out, and remains within the mainstream of his work, while being suited to a new form.

Then a full-time planning office will be established in Rome to work with a five-member Georgia Tech research staff for development of an area planning and industrial development program.

The Jackson report will provide some of the political support Mr. Rusk will need if he is to get rid of department personnel engaged, as Sen. Jackson puts it, `` in work that does not really need doing ''.

A driver of a dairy truck, who begins work at 1 a.m. finishes before breakfast, then goes out and grows a garden, and who has used the cannery to save and feed a family of five, asked, `` What in the world will we do ''??

No one will even suspect that it is your work ''.

The Government will contract with an eligible applicant to pay up to one-half of the cost of approved exploration work as it progresses.

Perhaps the most important incentive for them will be clear evidence that where other countries have done this kind of home work we have responded with long-term commitments.

The Armed Forces Epidemiological Board agreed to submit each month a report for one of its 12 commissions, so that each commission will report once a year on some phase of its work calculated to be of particular interest and value to medical officers of the Armed Forces.

With thousands of young Americans going to work in developing areas, millions of Americans will become more directly involved in the world than ever before.

To meet this urgent need for skilled manpower we are proposing the establishment of a Peace Corps -- an organization which will recruit and train American volunteers, sending them abroad to work with the people of other nations.

This organization will differ from existing assistance programs in that its members will supplement technical advisers by offering the specific skills needed by developing nations if they are to put technical advice to work.

The work will consist of: 1.

This work will cover the most serious one-fourth of all land needing such treatment, and will consist of burning 250,000 acres of highly hazardous debris concentration, felling snags on 350,000 acres of high lightning-occurrence areas, prescribed burning on 3.5 million acres, removing roadside fuel on 39,000 acres, and clearing and maintaining 11,000 miles of firebreaks.

Rodent control work for the 10-year period will be aimed at control of the most serious infestations of harmful rodents, such as porcupines and mice, on high-value areas of forage and commercial timberlands.

Approximately 40 percent of the value of the work on roads for access to timber which are planned for this period will be constructed by purchasers of National Forest timber, but paid for by the Government through adjustment of stumpage prices.

one B-52 can do the work of several B-47's which it will replace.