* Protein tag, describing addition of peptide sequences to enable protein isolation

Most such methods do rely on comparisons between the sequence to be predicted and sequences of known structure in the Protein Data Bank and thus account for environment indirectly, assuming the target and template sequences share similar cellular contexts.

* Protein – a class of biochemicals made from amino acids in specific sequences.

Protein function depends on 3-D structure and these 3-D structures are more highly-conserved than sequences.

* Protein, the NIH protein database, a collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB

Protein structural motifs often include loops of variable length and unspecified structure, which in effect create the " slack " necessary to bring together in space two elements that are not encoded by immediately adjacent DNA sequences in a gene.

Protein sequences can provide data about the biological function and evolution of proteins and protein domains.

Protein design involves identifying novel sequences within this subset, in particular those with a physiologically active native state.

Protein design can be accomplished using computer models, which, while simplifying the problem, are able to generate sequences that fold to the desired structure.

She initiated the collection of protein sequences in the Atlas of Protein Sequence and Structure, a book collecting all known protein sequences that she published in 1965.

This led to the Protein Information Resource database of protein sequences, which was developed by her group.

Prior to that, the NBRF compiled the first comprehensive collection of macromolecular sequences in the Atlas of Protein Sequence and Structure, published from 1965-1978 under the editorship of Margaret Dayhoff.

Protein tags are peptide sequences genetically grafted onto a recombinant protein.

Protein A, protein G, and protein L are examples of proteins that strongly bind to various antibody isotypes.

Protein sequence can be determined by Edman degradation, in which the N-terminal residues are hydrolyzed from the chain one at a time, derivatized, and then identified.

Low carbohydrate diets such as Atkins and Protein Power are relatively high in protein and fats.

Protein crystals are formed in salt solutions.

Solved structures are usually deposited in the Protein Data Bank ( PDB ), a freely available resource from which structural data about thousands of proteins can be obtained in the form of Cartesian coordinates for each atom in the protein.

Protein kinases are also found in bacteria and plants.

The images below are of Lipscomb's structures from the Protein Data Bank displayed in simplified form with atomic detail suppressed.

C: Microdialysis Protein crystals are almost always grown in solution.

Three-dimensional protein structures of the peroxidated catalase intermediates are available at the Protein Data Bank.

Some of the more recognized diets are Bourbon's Modified Leadbeaters ( BML ), High Protein Wombaroo ( HPW ), various calcium rich diets and Leadbeaters Mixture ( LBM ).

Protein formation and destruction are both accelerated in hyperthyroidism.

Protein kinases are a group of enzymes that possess a catalytic subunit that transfers the gamma ( terminal ) phosphate from nucleotide triphosphates ( often ATP ) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function.

There are over 100 3D structures of tyrosine kinases available at the Protein Data Bank.

Approximately 2000 kinases are known, and more than 90 Protein Tyrosine Kinases ( PTKs ) have been found in the human genome.

Protein tyrosine kinases that are encoded by the Rous sarcoma virus cause cellular transformation, and are termed oncoproteins.

* Protein splicing, a natural process where inteins are removed

Transpeptidases, such as the sortases responsible for anchoring factors like Protein A to the staphylococcal peptidoglycan, are being studied in hopes of developing new antibiotics to target MRSA infections.

In addition, structures of many water-soluble domains of IMPs are available in the Protein Data Bank.

Protein expression systems are very widely used in the life sciences, biotechnology and medicine.

Protein expression systems are used to produce certain proteins in biotechnology and industry, and more recently to produce sets ( combinatorial series ) of protein that are screened for drug discovery purpose.

Protein levels are evaluated through densitometry ( how intense the stain is ) or spectrophotometry.

Protein molecules — either of the host's own phenotype or of other biologic entities — are continually synthesized and degraded in a cell.

Protein are frequently described as consisting from several structural units.

Protein folding is routinely studied using NMR spectroscopy, for example by monitoring hydrogen-deuterium exchange of partially folded intermediates.

The Protein Crystal Growth experiment made its fourteenth shuttle flight, but USML-1 represented the first time crew members were able to optimize growth conditions using the Glovebox facility.

Protein analysis using microPIXE allow for the determination of the elemental composition of liquid and crystalline proteins.

The Protein Structure Initiative ( PSI ) is a multifaceted effort funded by the National Institutes of Health with various academic and industrial partners that aims to increase knowledge of protein structure using a structural genomics approach and to improve structure-determination methodology.

They also conducted a variety of mid-deck experiments, including the Microgravity Disturbance Experiment ( MDE ) using the Fluids Experiment Apparatus ( FEA ), Protein Crystal Growth ( PCG ), American Flight Echocardiograph ( AFE ), Latitude / Longitude Locator ( L3 ), Mesoscale Lightning Experiment ( MLE ), Characterization of Neurospora Circadian Rhythms ( CNCR ) and the IMAX camera.

They also operated a variety of middeck experiments including the Microgravity Disturbance Experiment ( MDE ) using the Fluids Experiment Apparatus ( FEA ), Protein Crystal Growth ( PCG ), American Flight Echocardiograph ( AFE ), Latitude / Longitude Locator ( L3 ), Mesoscale Lightning Experiment ( MLE ), Characterization of Neurospora Circadian Rhythms ( CNCR ), and the IMAX Camera.

During Mir-21 Onufriyenko performed numerous research experiments including Protein crystal growth experiments experiments in materials science using high temperature melting oven " Optizon ".

Native chemical ligation of this kind using a recombinant polypeptide segment is known as Expressed Protein Ligation.

Protein skimming removes certain organic compounds, including proteins and amino acids, by using the polarity of the protein itself.

At the end of culturing, the IgG contained in the medium is purified by an affinity-column using Protein A as the adsorbent, followed by chromatography steps, and finally concentrated by UF / DF ( paired ultra filtration / depth filtration ).

These fragments can often be purified or immobilized using Protein L, since Protein L interacts with the variable region of kappa light chains.

Protein microarrays were developed due to the limitations of using DNA microarrays for determining gene expression levels in proteomics.

Protein misfolding has previously been studied using both computational approaches as well as in vivo biological assays in model organisms such as Drosophila melanogaster and C. elegans.

Image made by me using the RalMol software and Protein Data Bank coordinates.