# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

# Reverse transcriptase is added, along with deoxynucleotide triphosphates ( A, T, G, C ).

Reverse transcriptase activity outside of retroviruses has been found in almost all eukaryotes, enabling the generation and insertion of new copies of retrotransposons into the host genome.

Reverse transcriptase was discovered by Howard Temin at the University of Wisconsin – Madison in RSV virions, and independently isolated by David Baltimore in 1970 at MIT from two RNA tumour viruses: R-MLV and again RSV.

Reverse transcriptase creates single-stranded DNA from an RNA template.

Reverse transcriptases are also found in bacterial Retron msr RNAs, distinct sequences which code for reverse transcriptase, and are used in the synthesis of msDNA.

Reverse transcriptase enzymes include an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase, which work together to perform transcription.

Reverse transcriptase has a high error rate when transcribing RNA into DNA since, unlike any other DNA polymerases, it has no proofreading ability.

Reverse transcriptase is commonly used in research to apply the polymerase chain reaction technique to RNA in a technique called reverse transcription polymerase chain reaction ( RT-PCR ).

Reverse transcriptase is used also to create cDNA libraries from mRNA.

Reverse transcriptase has also been employed in insulin production.

Reverse transcription is catalyzed by a reverse transcriptase, which is often coded by the TE itself.

Telomeres are extended by telomerases, part of a protein subgroup of specialized reverse transcriptase enzymes known as TERT ( TElomerase Reverse Transcriptases ) that are involved in synthesis of telomeres in humans and many other, but not all, organisms.

Reverse transcriptase is essential for the reproduction of retroviruses such as HIV and was also discovered independently, and at about the same time, by Mizutani and Temin.

* Reverse transcriptase, an enzyme that transcribes RNA to DNA

Reverse transcriptase is also an important component of several important techniques in molecular biology, such as the reverse transcription polymerase chain reaction, and diagnostic medicine.

# Redirect Reverse transcriptase

Reverse transcriptase polymerase chain reaction ( RT-PCR ) methods have been developed for detection of TSV and are very sensitive.

* Reverse Transcriptase, an enzyme used by RNA retroviruses like HIV, which is used to create a complementary strand to the preexisting strand of viral RNA before it can be integrated into the DNA of the host cell.

#* Reverse Transcription Polymerase Chain Reaction ( RT-PCR ) is a variation of PCR that can be used to quantify viral RNA.

About 300 samples were seeded from 34 protein types including HIV Reverse Transcriptase Complex ( an enzyme that is a chemical key to the replication of AIDS ) and Factor D ( an important enzyme in human immune systems ).

Reverse zymography copolymerizes both the substrate and the enzyme with the acrylamide, and is useful for the demonstration of enzyme inhibitor activity.

RPF is often incorrectly defined as Reverse Path Filtering, particularly when it comes to unicast routing.

If the meet is a 5 optional meet, then the divers will perform 1 optional from each category ( Front, Back, Inward, Reverse, and Twister ) and then 1 voluntary from the category of their choice.

If the door swings toward you, it is " Reverse swing "; or if the door swings away from you, it is " Normal swing ".

Reverse transcribing viruses replicate their genomes by reverse transcribing DNA copies from their RNA ; these DNA copies are then transcribed to new RNA.

Reverse osmosis desalinates fresh water from ocean salt water.

The group that wrote DeCSS, including Johansen, came to call themselves Masters of Reverse Engineering and may have obtained information from Drink or Die.

* 3rd mission ( Reverse Engineering ): The dam-busting climax is similar to " Force 10 from Navarone ".

The activity of retrodiction ( or postdiction ) involves moving backwards in time, step-by-step, in as many stages as are considered necessary, from the present into the speculated past, in order to establish the ultimate cause of a specific event ( e. g., Reverse engineering and Forensics ).

Reverse Lend-Lease comprised services such as rent on air bases that went to the U. S., and totaled $ 7. 8 billion ; of this, $ 6. 8 billion came from the British and the Commonwealth.

The Reverse Address Resolution Protocol ( RARP ) is an obsolete computer networking protocol used by a host computer to request its Internet Protocol ( IPv4 ) address from an administrative host, when it has available its Link Layer or hardware address, such as a MAC address.

Reverse ARP differs from the Inverse Address Resolution Protocol ( InARP ) described in RFC 2390, which is designed to obtain the IP address associated with a local Frame Relay data link connection identifier.

In 2004 — 2005, through the success of a group of BBC employees ' restoration work on similar NTSC-only episodes of Doctor Who, the BBC decided to convert all their NTSC-only productions ( as reclaimed from various international stations ) back to their original PAL format using a new computer-controlled process, Reverse Standards Conversion.

Reverse of a silver stater from Aspendos, Pamphylia.

Reverse engineering has also revealed the secrets of some synthesizer components, such as those from ARP Instruments, Inc.

Reverse of Svyatopolk's coin contains a fork image, interpreted as a sign of his descendance from two fathers.

It has been asserted that the stability of the economic recovery that took place in the United Kingdom under John Major's government from late 1992 onwards was a result of Reverse Goodhart's Law: that, if a government's economic credibility is sufficiently damaged, then its targets are seen as irrelevant and the economic indicators regain their reliability as a guide to policy.

* Reverse transcription-process viruses use to make DNA from RNA

* " Split Horizon " and " Split Horizon with Poison Reverse " in Convergence in RIP Internetworks, from Microsoft TechNet.

The curse had been such a part of Boston culture that when a road sign on the city's busy Storrow Drive was vandalized from " Reverse Curve " to " Reverse The Curse ," officials left it in place until after the Red Sox won the 2004 Series.