# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

# Reverse transcriptase is added, along with deoxynucleotide triphosphates ( A, T, G, C ).

Reverse transcriptase activity outside of retroviruses has been found in almost all eukaryotes, enabling the generation and insertion of new copies of retrotransposons into the host genome.

Reverse transcriptase, a viral enzyme that comes from the virus itself after it is uncoated, converts the viral RNA into a complementary strand of DNA, which is copied to produce a double stranded molecule of viral DNA.

Reverse transcriptase was discovered by Howard Temin at the University of Wisconsin – Madison in RSV virions, and independently isolated by David Baltimore in 1970 at MIT from two RNA tumour viruses: R-MLV and again RSV.

Reverse transcriptase creates single-stranded DNA from an RNA template.

Reverse transcriptases are also found in bacterial Retron msr RNAs, distinct sequences which code for reverse transcriptase, and are used in the synthesis of msDNA.

Reverse transcriptase enzymes include an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase, which work together to perform transcription.

Reverse transcriptase has a high error rate when transcribing RNA into DNA since, unlike any other DNA polymerases, it has no proofreading ability.

Reverse transcriptase is used also to create cDNA libraries from mRNA.

Reverse transcriptase has also been employed in insulin production.

Reverse transcription is catalyzed by a reverse transcriptase, which is often coded by the TE itself.

Telomeres are extended by telomerases, part of a protein subgroup of specialized reverse transcriptase enzymes known as TERT ( TElomerase Reverse Transcriptases ) that are involved in synthesis of telomeres in humans and many other, but not all, organisms.

Reverse transcriptase is essential for the reproduction of retroviruses such as HIV and was also discovered independently, and at about the same time, by Mizutani and Temin.

* Reverse transcriptase, an enzyme that transcribes RNA to DNA

Reverse transcriptase is also an important component of several important techniques in molecular biology, such as the reverse transcription polymerase chain reaction, and diagnostic medicine.

# Redirect Reverse transcriptase

Reverse transcriptase polymerase chain reaction ( RT-PCR ) methods have been developed for detection of TSV and are very sensitive.

The first was a radio receiver, such as the Icom PCR-1000, that could tune into the Reverse Channel, which is the frequency that the phones transmit data to the tower on.

If the meet is a 5 optional meet, then the divers will perform 1 optional from each category ( Front, Back, Inward, Reverse, and Twister ) and then 1 voluntary from the category of their choice.

If the door swings toward you, it is " Reverse swing "; or if the door swings away from you, it is " Normal swing ".

Reverse faults occur in areas where the crust is being shortened such as at a convergent boundary.

* The FREDFET ( Fast Reverse or Fast Recovery Epitaxial Diode FET ) is a specialized FET designed to provide a very fast recovery ( turn-off ) of the body diode.

* Locationless / Reverse: This variation is similar to a scavenger hunt.

The process is known as Reverse painting on glass.

It is known as a Reverse Roundhouse Kick because the kicker turns in the opposite, or " reverse ", direction before the kick is executed.

As is the case with Reverse Polish notation, although R1C1-style formulas are more efficient than A1-style formulae ( for instance, relative and absolute references can be mixed in the same expression, as " R1C " to mean " row 1, this column "), most spreadsheet users prefer the A1 addressing style introduced by VisiCalc.

* Reverse Transcriptase, an enzyme used by RNA retroviruses like HIV, which is used to create a complementary strand to the preexisting strand of viral RNA before it can be integrated into the DNA of the host cell.

The term Polish notation is sometimes taken ( as the opposite of infix notation ) to also include Polish postfix notation, or Reverse Polish notation, in which the operator is placed after the operands.

Reverse Polish notation ( RPN ) is a mathematical notation wherein every operator follows all of its operands, in contrast to Polish notation, which puts the operator in the prefix position.

Reverse logistics is the process of managing the return of goods.

Reverse logistics is also referred to as " Aftermarket Customer Services ".

Reverse Camber is a term that describes skis with something other than the traditional camber shape.

Reverse camber skis have tips and tails that curve up while the length between them is flat.

Reverse domain name hijacking is most commonly perpetrated by larger corporations and famous individuals.

Reverse convertibles securities are most commonly targeted towards the US market.

Based on Hoshino's work, Kaya Kizaki has written two novels, commonly named D. Gray-man Reverse, and Konami has developed two video games and a trading card game.

Reverse video is commonly used in software programs as a visual aid to highlight a selection that has been made as an aid in preventing description errors, where an intended action is performed on an object that is not the one intended.

He has pioneered or popularised the use of many techniques in computer graphics, including " adaptive tile refresh " for Commander Keen, raycasting for Hovertank 3-D, Catacomb 3-D, and Wolfenstein 3-D, binary space partitioning which Doom became the first game to use, surface caching which he invented for Quake, Carmack's Reverse ( formally known as z-fail stencil shadows ) which he devised for Doom 3, and MegaTexture technology, first used in Enemy Territory: Quake Wars.

Sirens are sometimes used as part of an integrated warning system that links sirens with other warning media such as the radio and TV Emergency Alert System, NOAA Weather Radio, telephone alerting systems, Reverse 911, Cable Override and wireless alerting systems in the United States and the Emergency Public Warning System in the Canadian province of Alberta.

* Reverse Circulation, a term used in drilling rig

* Reverse engineer proprietary protocols used over the network

The Reverse Address Resolution Protocol ( RARP ) is an obsolete computer networking protocol used by a host computer to request its Internet Protocol ( IPv4 ) address from an administrative host, when it has available its Link Layer or hardware address, such as a MAC address.

Reverse appliqué techniques are often used in combination with traditional appliqué techniques, to give a variety of visual effects.

Reverse slash is a term used for fanfic without any sexual content, or very little sexual content, compared to the canon.

Reverse discrimination may also be used to highlight the discrimination inherent in affirmative action programs.

Reverse telephone directories are used by law enforcement and other emergency services to determine the origin of any request for assistance.

* Parabolic SAR Stop and Reverse indicator, used in technical analysis stock analysis

Reverse conducting thyristors are often used in frequency changers and inverters.

Reverse folds of a corner are typically used to produce feet or birds heads.

Reverse thrust is used by many jet aircraft to help slow down just after touch-down, redirecting engine exhaust forward instead of back.

#* Reverse Transcription Polymerase Chain Reaction ( RT-PCR ) is a variation of PCR that can be used to quantify viral RNA.

Early automatic transmissions, including GM's Hydra-Matic, Packard's Ultramatic, and Borg Warner's automatic used by a number of independent manufacturers ( Rambler, Studebaker ) used a pattern of " P N D L R " which put Reverse at the bottom of the quadrant, next to Low.

Chevrolet's Powerglide, at least as seen on the Corvair, used a " R N D L " pattern which separated the Reverse from the Drive gears by neutral in the ideal way, but which had no " P " selection, relying instead on a separate hand brake when parking.

Although UDRP panelists currently have no tools by which to punish abuses such as Reverse Domain Name Hijacking, such a finding might be used in a local jurisdiction where such abuses might constitute a tort such as tortious interference with contract or an unfair business practice.

It can also be used to secure access to internal web servers ( Reverse proxy ).

It has been popularised by the video game Doom 3, and a particular variation of the technique used in this game has become known as Carmack's Reverse ( see depth fail below ).