# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

# Reverse transcriptase is added, along with deoxynucleotide triphosphates ( A, T, G, C ).

Reverse transcriptase activity outside of retroviruses has been found in almost all eukaryotes, enabling the generation and insertion of new copies of retrotransposons into the host genome.

Reverse transcriptase, a viral enzyme that comes from the virus itself after it is uncoated, converts the viral RNA into a complementary strand of DNA, which is copied to produce a double stranded molecule of viral DNA.

Reverse transcriptase was discovered by Howard Temin at the University of Wisconsin – Madison in RSV virions, and independently isolated by David Baltimore in 1970 at MIT from two RNA tumour viruses: R-MLV and again RSV.

Reverse transcriptase creates single-stranded DNA from an RNA template.

Reverse transcriptases are also found in bacterial Retron msr RNAs, distinct sequences which code for reverse transcriptase, and are used in the synthesis of msDNA.

Reverse transcriptase has a high error rate when transcribing RNA into DNA since, unlike any other DNA polymerases, it has no proofreading ability.

Reverse transcriptase is commonly used in research to apply the polymerase chain reaction technique to RNA in a technique called reverse transcription polymerase chain reaction ( RT-PCR ).

Reverse transcriptase is used also to create cDNA libraries from mRNA.

Reverse transcriptase has also been employed in insulin production.

Reverse transcription is catalyzed by a reverse transcriptase, which is often coded by the TE itself.

Telomeres are extended by telomerases, part of a protein subgroup of specialized reverse transcriptase enzymes known as TERT ( TElomerase Reverse Transcriptases ) that are involved in synthesis of telomeres in humans and many other, but not all, organisms.

Reverse transcriptase is essential for the reproduction of retroviruses such as HIV and was also discovered independently, and at about the same time, by Mizutani and Temin.

* Reverse transcriptase, an enzyme that transcribes RNA to DNA

Reverse transcriptase is also an important component of several important techniques in molecular biology, such as the reverse transcription polymerase chain reaction, and diagnostic medicine.

# Redirect Reverse transcriptase

Reverse transcriptase polymerase chain reaction ( RT-PCR ) methods have been developed for detection of TSV and are very sensitive.

The term Polish notation is sometimes taken ( as the opposite of infix notation ) to also include Polish postfix notation, or Reverse Polish notation, in which the operator is placed after the operands.

Other variations include: Pocky G ( marketed as being " hard and rich "), Giant Pocky ( Strawberry and Chocolate flavored ; each box contains 20 individually wrapped sticks with real dried strawberry ; each stick is about 10 " long, and approximately 3 times the diameter of a normal Pocky stick ), Reverse Pocky ( cracker on the outside with the filling in the middle ), Fortune-Telling Pocky ( each stick contained a " fortune ") and Pocky Cake ( a literal cake shaped to look like a Pocky stick.

The source does not include any of the ' Carmack's Reverse ' code.

His other books include Ray in Reverse and The Watermelon King.

His other books include Ray in Reverse, The Watermelon King, and Mr. Sebastian and the Negro Magician.

* Reverse Transcriptase, an enzyme used by RNA retroviruses like HIV, which is used to create a complementary strand to the preexisting strand of viral RNA before it can be integrated into the DNA of the host cell.

Reverse transcribing viruses replicate their genomes by reverse transcribing DNA copies from their RNA ; these DNA copies are then transcribed to new RNA.

* Reverse transcription-process viruses use to make DNA from RNA

Reverse genetics is an approach to discover the function of a gene by analyzing the phenotypic effects of specific gene sequences obtained by DNA sequencing.

Reverse transcription polymerase chain reaction ( RT-PCR ) is a variant of polymerase chain reaction ( PCR ).

Reverse transcription PCR is not to be confused with real-time polymerase chain reaction ( Q-PCR / qRT-PCR ), which is also sometimes abbreviated as RT-PCR.

# REDIRECT Reverse transcription polymerase chain reaction

The first was a radio receiver, such as the Icom PCR-1000, that could tune into the Reverse Channel, which is the frequency that the phones transmit data to the tower on.

There are six " groups " into which dives are classified: Forward, Back, Inward, Reverse, Twist, and Armstand.

It has been speculated that this template switching activity of Reverse Transcriptase, which can be demonstrated completely in vivo, may have been one of the causes for finding several thousand unannotated transcripts in the genomes of model organisms.

Reverse Polish notation ( RPN ) is a mathematical notation wherein every operator follows all of its operands, in contrast to Polish notation, which puts the operator in the prefix position.

He has pioneered or popularised the use of many techniques in computer graphics, including " adaptive tile refresh " for Commander Keen, raycasting for Hovertank 3-D, Catacomb 3-D, and Wolfenstein 3-D, binary space partitioning which Doom became the first game to use, surface caching which he invented for Quake, Carmack's Reverse ( formally known as z-fail stencil shadows ) which he devised for Doom 3, and MegaTexture technology, first used in Enemy Territory: Quake Wars.

Reverse electron transitions for all these types of excited molecules are also possible to return to their ground states, which can be designated as σ *→ σ, π *→ π, or π *→ n.

Reverse ARP differs from the Inverse Address Resolution Protocol ( InARP ) described in RFC 2390, which is designed to obtain the IP address associated with a local Frame Relay data link connection identifier.

* Reverse auction is a type of auction in which the roles of the buyer and the seller are reversed, with the primary objective to drive purchase prices downward.

* Reverse proxy which discusses origin-side proxies

Reverse speech is a pseudoscience first advocated by David John Oates which gained publicity when it was mentioned on Art Bell's nightly Coast to Coast AM radio talk show.

Reverse osmosis plant membrane systems typically use less energy than thermal distillation, which has led to a reduction in overall desalination costs over the past decade.

* Reverse proxy which discusses origin-side proxies

* Reverse proxy which discusses origin-side proxies

Even though Jerry shows Elaine's " Christmas Card " in " The Pick ", he reveals his feelings in " The Soul Mate " until " The Reverse Peephole " in which Elaine tries to get the fur coat but he is already in love with Svetlana.

The launch show, which was live and an hour long, was notable for a performance of " Flip Reverse " by Blazin ' Squad, featuring hordes of hooded teenagers choreographed to dance around the outside of BBC Television Centre.