" Most agarose gels are made with between 0. 7 % ( good separation or resolution of large 5 – 10kb DNA fragments ) and 2 % ( good resolution for small 0. 2 – 1kb fragments ) agarose dissolved in electrophoresis buffer.

Conversely, S. pombe has large, repetitive centromeres ( 40 – 100 kb ) more similar to mammalian centromeres, and degenerate replication origins of at least 1kb.

The DNA is looped out to search for the nearest d ( GATC ) methylation site to the mismatch, which could be up to 1kb away.

The only hope which good teachers have for being paid their due is to stop dragging the dead weight of poor teachers up the economic ladder with them.

Our pools also have wide, shallow steps -- for the benefit of the littlest swimmers who can thus be introduced to the water with far greater safety than a ladder affords.

He was interrupted by a crash from the deck and sprang toward the ladder, with Gansevoort and Heiser behind him.

They ignored the ladder down to the planet surface and, with only a glance at the seismological gauge to judge surface resistance, dropped to the ground.

Guards watched throughout the night, except at Alexander's bedchamber, which was at the top of a ladder with a ferocious chained dog guarding the door.

Thebe, Alexander's wife and cousin ( or half-sister, as the daughter of Jason of Pherae ), concealed her three brothers in the house during the day, had the dog removed when Alexander had gone to rest, and, having covered the steps of the ladder with wool, brought up the young men to her husband's chamber.

In the 1923 season the Dons topped the ladder with 13 wins from 16 games.

In 1946, Essendon were clearly the VFL's supreme force, topping the ladder after the roster games and surviving a drawn second semi final against Collingwood to win through to the grand final a week later with a 10. 16 ( 76 ) to 8. 9 ( 57 ).

In 1989, they rebounded further to second on the ladder with only five losses and thrashed Geelong in the Qualifying Final.

Essendon would finish 2008 in 12th position on the AFL ladder with 8 wins and 14 losses.

As of Round 14, the Bombers sit 4th on the ladder on percentage with 10 wins and 3 losses.

Movies with budgets a step up the ladder, known as " intermediates " within the industry, might be treated as A or B pictures depending on the circumstance — Monogram created a new unit, Allied Artists, in the late 1940s to focus on this sort of production.

Synagogue of Prague with the rungs of the ladder to the attic on the wall.

In particular, Jahn crafted early models of the horizontal bar, the parallel bars ( from a horizontal ladder with the rungs removed ), and the vaulting horse.

It said the Fathers saw foreshadowings of Mary's " wondrous abundance of divine gifts and original innocence " " in that ark of Noah, which was built by divine command and escaped entirely safe and sound from the common shipwreck of the whole world ; in the ladder which Jacob saw reaching from the earth to heaven, by whose rungs the angels of God ascended and descended, and on whose top the Lord himself leaned ; in that bush which Moses saw in the holy place burning on all sides, which was not consumed or injured in any way but grew green and blossomed beautifully ; in that impregnable tower before the enemy, from which hung a thousand bucklers and all the armor of the strong ; in that garden enclosed on all sides, which cannot be violated or corrupted by any deceitful plots ; in that resplendent city of God, which has its foundations on the holy mountains ; in that most august temple of God, which, radiant with divine splendours, is full of the glory of God ; and in very many other biblical types of this kind.

Near Luz en route to Haran, Jacob experienced a vision of a ladder, or staircase, reaching into heaven with angels going up and down it, commonly referred to as " Jacob's ladder ".

Icon of The Ladder of Divine Ascent showing monks ascending ( and falling from ) the ladder to heaven, with Jesus at the upper right helping them.

Also shown are angels helping the climbers, and demons attempting to shoot with arrows or drag down the climbers, no matter how high up the ladder they may be.

Often, in the lower right corner St. John Climacus himself is shown, gesturing towards the ladder, with rows of monastics behind him.

The Knights are led by a man who is approximately 12 feet tall with disproportionately short arms and reindeer antlers inserted into his helmet ( played by Michael Palin standing on a ladder ; the original screenplay suggested that he be played by " Mike standing on John's shoulders ").

In 1949 North secured the VFL Minor Premiership, finishing top of the ladder at the end of the home-and-away season with 14 wins and 5 losses.

In the end, after killing some bandits in the underground, you would go out to Agropom via the underground's ventilation stairs to a ladder to a ventilation access gate, in the way through the stairs, the two poltergeist would attack the player with fire beams.

The chokes from behind were usually accompanied with a grapevine body lock ( ἄγχειν μετὰ κλιμακισμοῦ — anchein meta klimakismou " choking with the ladder trick "), as the resulting stretch of the body of the opponent accentuated the effect of the choke.

The cleavage and inactivation of ICAD / DFF45 by a caspase allows CAD to enter the nucleus and fragment the DNA, causing the characteristic ' DNA ladder ' in apoptotic cells.

There was no place to sit, but Watson walked slowly from the ladder to the window slits and back, stooping slightly to avoid striking his head on the heavy beams.

There would seem to be some small solace in the prospect that the missile race between nations is at the same time accelerating the study of the space around us, giving us a long-sought ladder from which to peer at alien regions.

Within a season the Lions rose from the bottom of the ladder to 4th, before losing to the eventual Premiers the Kangaroos in the Preliminary Final.

The conspiracy took its name from a torture method, in which blacks were tied to a ladder and whipped until they confessed or died.

A Republican lawyer from Vermont, Coolidge worked his way up the ladder of Massachusetts state politics, eventually becoming governor of that state.

Another decree in 1853 from the sultan solidified the existing territorial division among the communities and set a status quo for arrangements to " remain forever ", caused differences of opinion about upkeep and even minor changes, including disagreement on the removal of an exterior ladder under one of the windows ; this ladder has remained in the same position since then.

A further premiership in 1965 ( won from 4th position on the ladder ), was also unexpected due to periods of poor form during the season.

In 1983, the Bombers moved upwards following a slow start to be second after thirteen rounds, but then suffered a five-match slump before recovering to reach their first grand final for 15 years from fourth place on the ladder.

He heard the voice of God, who repeated many of the blessings upon him, coming from the top of the ladder ..

An icon known by the same title, Ladder of Divine Ascent, depicts a ladder extending from earth to heaven ( cf.

Due to the legitimate role that referees play in wrestling of serving as liaison between the bookers backstage and the wrestlers in the ring ( the role of being a final arbitrator is merely kayfabe ), the referee is present, even in matches that do not at first glance appear to require a referee ( such as a ladder match, as it is no holds barred, and the criteria for victory could theoretically be assessed from afar ).

Modern PLCs can be programmed in a variety of ways, from the relay-derived ladder logic to programming languages such as specially adapted dialects of BASIC and C. Another method is State Logic, a very high-level programming language designed to program PLCs based on state transition diagrams.

Because the Swans and the Saints used to be towards the bottom of the ladder on a regular basis after the Second World War up until St Kilda's move away from the Junction Oval, clashes between both sides used to be dubbed " The Lake Premiership ".

At the very end of the text he borrows an analogy from Arthur Schopenhauer, and compares the book to a ladder that must be thrown away after one has climbed it.

The police pursue THX up an escape ladder, but are ordered by central command to cease pursuit, mere steps away from capturing him, as the expense of his capture exceeds their pre-determined budget.

In some cases, courtesans were from well-to-do backgrounds, and were even married — but to husbands lower on the social ladder than their clients.