3-The mobile plasmid is nicked and a single strand of DNA is then transferred to the recipient cell.

The 5 ′ end of the nicked strand is transferred to a tyrosine residue on the nuclease and the free 3 ′ OH group is then used by the DNA polymerase for new strand synthesis.

3-The mobile plasmid is nicked and a single strand of DNA is then transferred to the recipient cell.

3-The mobile plasmid is nicked and a single strand of DNA is transferred to the recipient cell.

They contain a cosN site where DNA is nicked at each strand, 12bp apart, by terminase.

The DNA is nicked and the 3 ’ end serves as a primer.

This may be interpreted as a kind of wabi-sabi aesthetic, further confirmed by the way the colour of glazed items is known to change over time as hot water is repeatedly poured into them ( sabi ) and the fact that tea bowls are often deliberately chipped or nicked at the bottom ( wabi ), which serves as a kind of signature of the Hagi-yaki style.

In British slang terminology, the term " nicked " is often synonymous with being arrested, and " nick " can also refer to a police station, and the term " pinched " is also common.

* Members of the East Bengal Regiment of the Bangladesh Army are nicked as ' Bengal Tigers ' and the regiment logo is a tiger face.

A Bryde's whale in False Bay, South Africa, showing upright dorsal fin, which is often nicked or frayed on its trailing edge ( shown here )

* The most common dismissal effected by the keeper is for him to catch a ball that has nicked the batsman's bat, called an edge, before it bounces.

The reaction generates a nicked, circular DNA which is relaxed.

* The nonic, a variation on the conical design, where the glass bulges out a couple of inches from the top ; this is partly for improved grip, partly to prevent the glasses from sticking together when stacked, and partly to give strength and stop the rim from becoming chipped or " nicked ".

" This is followed by replacement in nicked sites by DNA polymerase I, which elongates the 3 ' hydroxyl terminus, removing nucleotides by 5 '- 3 ' exonuclease activity, replacing them with dNTPs.

2008: Henry Ford is the first hospital in southeastern Michigan to perform a new, minimally invasive procedure for back pain that spares the nerves from being nicked and back muscles from being cut.

DNA ligase then forms a phosphodiester bond to seal the resulting nicked duplex product, which now includes a new, correct cytosine.

The seed resembles a small, brownish nut, and should be nicked with a file and then soaked overnight before planting.

The following day in a match against Augsburg that finished 1 – 1, Jones was accused of cheating because of rolling on the ground and clutching his face following a high challenge that only nicked his hand ; his play acting was then compared to Rivaldo's infamous effort against Turkey at the World Cup in 2002.

She then nicked her ear with a pair of nail scissors and rejoined the others, playing the part of perplexed host.

I don't think anybody consciously nicked it from anybody else.

It was latter disclosed that they nicked the idea for the name from the BBC.

He stated that there was no return fire, and that Kerry was " nicked " by a fragment from an M-79 grenade launcher he fired himself.

The image of the unstoppable wave of transformation was nicked from Star Trek movie The Wrath of Khan: it's the Genesis device, slowed down, and once I had that, it became a rich source of metaphors: for colonialism, new technology, globalisation, change, death.

In the 2005 VH1 Storytellers program featuring Green Day, Billie Joe Armstrong stated that the title of the song was " nicked " from the famous Gottfried Helnwein painting of the same title, which features James Dean.

Pol II differs from Pol I in that it lacks a 5 '-> 3 ' exonuclease activity, and cannot use a nicked duplex template.

Fly cutters can fool you into putting your hand too close to the tool, so if you want to avoid nicked fingers, keep your hands well out of the way.

However, because of the large, scarred and nicked dorsal fins resembling those of mammal-hunting transients, they may also eat mammals and sharks.

" To this day, you ’ ve remained one of my best angels, and not just mine, but all of ours who, week after week, trust that our nicked and ragged selves, however hard we try to press them, will somehow serve to bring God ’ s truth to life.

Total hCG, C-terminal peptide total hCG, intact hCG, free ß-subunit hCG, ß-core fragment hCG, hyperglycosylated hCG, nicked hCG, alpha hCG, pituitary hCG.

He slew their guards and slipped into the camp, but as he was cutting Túrin's fetters, the sword nicked Túrin, waking him.

' You're fucking nicked, my old beauty ,' he said, and gave me a terrific clout on the ear.

The shot nicked her collar bone but she did not suffer permanent harm.

After one battle where he was nicked by a bullet, Cohen started carrying a second gun.

Lennon initially penned the lyrics, " You probably pinched that bitch anyway ", as a reference to the many times McCartney himself had made claims that he wasn't sure if he " nicked " Yesterday, having asked Lennon, Harrison, Martin and others if they heard that song before.

By convention, the top strand is written from the 5 ' end to the 3 ' end ; thus, the bottom strand is written 3 ' to 5 '.

: The corresponding RNA sequence, in which uracil is substituted for thymine where uracil takes its place in the RNA strand:

The remaining strand is replicated either independent of conjugative action ( vegetative replication beginning at the oriV ) or in concert with conjugation ( conjugative replication similar to the rolling circle replication of lambda phage ).

A beta strand ( also β strand ) is a stretch of polypeptide chain typically 3 to 10 amino acids long with backbone in an almost fully extended conformation.

* Citizenship is not taught as a discrete subject in Scottish schools, but is a cross-curricular strand of the Curriculum for Excellence.

# A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA ( Reverse transcriptase requires this double-stranded segment as a primer to start its operation.

This strand of DNA is complementary DNA.

The backbone of the DNA strand is made from alternating phosphate and sugar residues.

In a double helix the direction of the nucleotides in one strand is opposite to their direction in the other strand: the strands are antiparallel.

As a result of this complementarity, all the information in the double-stranded sequence of a DNA helix is duplicated on each strand, which is vital in DNA replication.

The denaturation of nucleic acids such as DNA due to high temperatures is the separation of a double strand into two single strands, which occurs when the hydrogen bonds between the strands are broken.

Dialectical materialism is a strand of Marxism, synthesizing Hegel's dialectics, which proposes that every economic order grows to a state of maximum efficiency, while simultaneously developing internal contradictions and weaknesses that contribute to its systemic decay.

The double helix is unwound and each strand acts as a template for the next strand.