The serum was measured volumetrically and subsequently dialyzed in the cold for at least 24 hr against three to four changes, approximately 750 ml each, of `` starting buffer ''.

He subsequently wrote three novels merging these overarching themes, The World of Null-A and The Pawns of Null-A in the late 1940s, and Null-A Three in the early 1980s.

During the Fourth Test news broke that prominent England players had agreed to take part in a " rebel tour " of South Africa the following winter ; three of them ( Tim Robinson, Neil Foster and John Emburey ) were playing in the match, and were subsequently dropped from the England side.

He fell to the ground " (), the light was " brighter than the sun " () and he was subsequently blinded for three days ().

" Many scholars argue that this collection originally consisted of three independent and anonymous prophecies, two of which were subsequently appended to the book of Zechariah ( as what scholars refer to as Deutero-Zechariah ) with the third becoming the book of Malachi.

She subsequently gave birth to three daughters and another son, Samuel ( who would eventually succeed their father as rector of Stenbrohult and write a manual on beekeeping ).

However, he subsequently produced three other proofs, the last one in 1849 being generally rigorous.

The identities of three of these were subsequently revealed:

The Soviets withdrew from the ACC in 1948 ; subsequently as the other three zones were increasingly unified and granted self-government, the Soviet administration facilitated the development of a separate socialist government in its zone.

The facility was named Kamakaiwi Field after James Kamakaiwi, a young Hawaiian who had arrived with the first group of four colonists, was subsequently picked as leader and spent a total of over three years on Howland, far longer than the average recruit.

Other folios and quartos were subsequently published — including John Smethwick's Q3, Q4, and Q5 ( 1611 – 37 )— but these are regarded as derivatives of the first three editions.

In AD 867 the Vikings seized Northumbria, forming the Kingdom of York ; three years later they stormed the Britons ’ fortress of Dumbarton and subsequently conquered much of England except for a reduced kingdom of Wessex, leaving the new combined Pictish and Gaelic kingdom almost encircled.

Zara and Fell subsequently divorced, she married Holt in 1946 and he adopted the three boys.

Other electors were added in the 17th century, including the Duke of Bavaria ( referred to as the Elector of Bavaria — replacing the Count Palatine of the Rhine, who was of the same family but had lost his title and vote temporarily during the Thirty Years ' War ), and the Duke of Brunswick-Lüneburg ( the Elector of Hanover-an office subsequently held by three Hanoverian kings of Great Britain, George I, George II, and George III ).

In the subsequent deputies ' elections, four JDA candidates were successful, but three of them subsequently left the party and continued to sit as independents.

He composed several instrumental sonatas with piano, including three for violin, two for cello, and two for clarinet ( which were subsequently arranged for viola by the composer ).

After Danish and Swedish troops in 1389 defeated the Swedish king, Albert of Mecklenburg, and he subsequently failed to pay the required tribute of 60, 000 silver marks within three years after his release, her position in Sweden was secured.

In his article, " Gray Barker: My Friend, the Myth-Maker ," John C. Sherwood claims that at age 18, he cooperated when Gray Barker urged him in the late 1960s to develop a hoax – which Barker subsequently published – about what Barker called " blackmen ", three mysterious UFO inhabitants who silenced Sherwood's pseudonymous identity, " Dr. Richard H. Pratt ".

In Zinder, a 2009 local newspaper report claimed there were no more than " three to five " automobile taxis operating in a diffuse city which subsequently relies upon the only partially regulated motorcycle taxi sector.

It has subsequently been reissued in three separate volumes in some countries, including in French and Spanish translations.

It was originally published in three volumes of two or three books each – Quicksilver ( 2003 ), The Confusion ( 2004 ) and The System of the World ( 2004 ) – but was subsequently republished as eight separate books: Quicksilver, King of the Vagabonds, Odalisque, Bonanza, Juncto, Solomon's Gold, Currency, and System of the World.

This would subsequently be interpreted as a difference in ages of the three clusters.

Smith started Game 1 and subsequently registered three putouts and one assist in the field, but went hitless in four at-bats in the Cardinals ' 4 – 2 loss.

On 21 July 2004, in the Delivering Security in a Changing World review of defence spending, Defence Secretary Geoff Hoon announced that three frigates of the fleet of sixteen would be paid off as part of a continuous cost-cutting strategy, and these were subsequently sold to Chile.

The song began with the chorus, which all of them sang with each one alternating ( in lip-synch ) a mournful howl after each of the first three lines.

The Sirens lived on three small, rocky islands called Sirenum scopuli and sang beautiful songs that enticed sailors to come to them, which resulted in the crashing of their ship into the islands.

Hell also co-wrote and sang lead vocals on the song " Never Mind " by The Heads, a 1996 collaborative effort between three former members of Talking Heads.

Robertson is credited as writer or co-writer for the majority of The Band's songs, but sang lead vocals on only three of their studio recordings (" To Kingdom Come ", " Knockin ' Lost John " and " Out Of The Blue ").

On the day of his death he woke up very early in the morning, went to chapel and meditated for three hours, sang a song on Kali and then he whispered, " If there were another Vivekananda, then he would have understood what this Vivekananda has done!

The three sang solos and duets, a family friend designed the album cover, and it was released on Veritas Records that same year as Folksingers ' Round Harvard Square.

Nico sang lead vocals on three songs (" Femme Fatale ", " All Tomorrow's Parties ", " I'll Be Your Mirror ") and backing vocal on " Sunday Morning ", on the band's debut album, The Velvet Underground & Nico ( 1967 ).

He wrote " Simple Song of Freedom " in 1969, which was recorded by Tim Hardin, who sang only three of the song's four verses.

He cut three obscure singles on CBS Records and sang with a variety of bands, including The Crawling King Snakes, which brought him into contact with drummer John Bonham.

She sang a total of three other roles at the Met: Ilia in Mozart's Idomeneo ( in its Metropolitan Opera premiere ), Tatiana in Tchaikovsky's Eugene Onegin, and Micaëla in Bizet's Carmen, the role of her final performance with the company on March 26, 1987.

According to Elaine Dundy, Presley sang " Love Me Tender " " straight, subdued and tender ... — a very different Elvis from the one on The Steve Allen Show three months before ".

The three aliens sang a song called " Under the Ink Black Sky ", which Pope wrote and performed.

They sang three songs: " Jive Talkin '", " To Love Somebody " and " You Should Be Dancing ".

He sang on and guest-hosted The Tonight Show, was involved in the production of Bill Cosby's first three albums, appeared in the Macy's Thanksgiving Day Parade, and sang " The Dropouts ' March " on the March 6, 1964, edition of the NBC-TV satirical program That Was The Week That Was.

At his mother's urging, Kravitz joined the California Boys Choir for three years, where he performed a classical repertoire, and sang with the Metropolitan Opera.

At the ceremony in the Waldorf-Astoria Hotel in New York, the three surviving members sang a medley of songs after being presented the awards by Merry Clayton, Marianne Faithfull, and Darlene Love.

Simon and Taylor also sang backup on three songs on John Hall's debut solo album John Hall, " The Fault ", " Good Enough " and " Voyagers ".

No matter the performance venue, plays were performed by, at most, three actors, who wore masks to identify them with the characters they portrayed ; they were accompanied by a chorus who sang and danced.

In Lund, he formed a group with A. E. Christernin and J. Meurling, together with whom he played and sang a type of three part music pieces.

In high school, Zander sang for three years in the Madrigals, the most demanding of the three choral groups at his school, played basketball and football, and had a part-time job at a sandwich shop.

Most of his main characters in all three shows sang songs at one time or another.

During the 2009 NHL Winter Classic at Wrigley Field, as the Chicago Blackhawks hosted the Detroit Red Wings on New Year's Day 2009, former Blackhawks players Bobby Hull, Stan Mikita, and Denis Savard and former Cubs players Ryne Sandberg and Ferguson Jenkins sang a hockey-themed version of the seventh-inning stretch ; " Take Me Out to the Hockey Game " used lines such as " Root, root, root for the Blackhawks " and " One, two, three pucks, you're out.

During the recording, Bowie sang each verse progressively louder than the last and as he increased volume in each verse, Visconti opened up each of the three microphones in turn, from closest to farthest.