A red filter, Zeiss barrier filter with the code ( Schott ) designation BG 23, was also used in the ocular lens assembly as it improved the contrast between specific and nonspecific fluorescence.

Such sections showed bright yellow-green specific fluorescence in the cells of the pseudophloem tissue ( Lee and Black, 1955 ).

In all the above procedures, when the intermediate sera were diluted to 1: 10 or 1: 100 with 0.85% saline, the specific and nonspecific fluorescence were not appreciably reduced, whereas, a dilution of the intermediate sera to 1: 500 or diluting the Af to 1: 5 greatly reduced specific fluorescence.

In all cases a disturbing amount of nonspecific staining was still present although it was still distinguishable from specific fluorescence.

* FRET Fluorescence resonance energy transfer is used to study protein interactions, detect specific nucleic acid sequences and used as biosensors, while fluorescence lifetime ( FLIM ) can give an additional layer of information.

Other advanced techniques, such as nonradioactive in situ hybridization, can be combined with immunochemistry to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification ( especially alkaline phosphatase and tyramide signal amplification ).

Monoclonal antibodies, specific to the virus, are also used for detection, as in fluorescence microscopy.

The term fluorescence is applied to phenomena in which the absorption of radiation of a specific energy results in the re-emission of radiation of a different energy ( generally lower ).

Phosphorescent powder under visible light, ultraviolet light, and total darkness. Phosphorescence is a specific type of photoluminescence related to fluorescence.

Rhodamine is a protein specific fluorescent stain commonly used in fluorescence microscopy.

In 2010 the United States National Institute of Allergy and Infectious Diseases recommended that the RAST test measurements of specific immunoglobulin E for the diagnosis of allergy be abandoned in favor of testing with mores more sensitive fluorescence enzyme-labeled assays.

They range from Plant Science based, which are used extensively to detect, measure and analyse fluorescence from chlorophyll during the photosynthesis process, to general purpose fluorometers tuned to the specific fluorescence porperties of target samples.

X-ray fluorescence spectroscopy can confirm the presence of bromine ( Br ), but it does not indicate the BFR concentration or specific molecule.

In practice, this means that a fluorescence peak is anchored at a specific frequency, whereas a Raman peak maintains a constant separation from the excitation frequency.

Some producers include multi-frequency fluorescence, such that different elements fluoresce under specific frequencies of light.

* Phosphorescence, a specific type of photoluminescence related to fluorescence

In a given oxygen concentration there will be a specific number of O < sub > 2 </ sub > molecules colliding with the film at any given time, and the fluorescence properties will be stable.

In the life sciences fluorescence microscopy is a powerful tool which allows the specific and sensitive staining of a specimen in order to detect the distribution of proteins or other molecules of interest.

Unlike transmitted and reflected light microscopy techniques fluorescence microscopy only allows observation of the specific structures which have been fluorescently labeled.

The second specific comment was the report of Eisenhower's Commission on National Goals, titled Goals For Americans.

No, Kayabashi was bringing his associates here for a specific purpose and Rector would not be able to fathom it until they arrived.

Counsel for the Government invited Du Pont's views on this proposal before recommending a specific program, but stated that if the court desired, or if counsel for Du Pont thought further discussion would not be profitable, the Government was prepared to submit a plan within thirty days.

Assuming thermal equilibrium between the anode surface and the transpiring argon, the gas enthalpy rise through the anode was calculated according to the relation Af whereby the specific heat of argon was taken as Af.

When 1 ml of conjugate was passed through a column ( Af ), the first and second milliliter fractions collected were the most specific and gave no nonspecific staining in some experiments, and very little in others.

After these treatments the conjugate did not stain healthy or crown gall sweet clover tissues or stained them a very faint green which was easily distinguishable from the bright yellow-green specific staining.

These conjugates Af had much less nonspecific staining than the previous conjugate ( with 50 mg FITC per gram of globulin ) while the specific staining was similar in both cases.

After passing Af through DEAE-cellulose, the titer of antibodies to WTV in the specific fraction was 1: 4 of the titer before such passage ( precipitin ring tests by R. F. Whitcomb ) ; ;

In our work the best procedure for removing substances causing nonspecific staining in order to obtain specific conjugates was to pass the conjugates through a DEAE-cellulose column and in some cases to absorb the first and second milliliter fractions with sweet clover tissue powder.

The specific staining by both direct and indirect methods showed that WTV antigen was concentrated in the pseudophloem tissue and in a few thick-walled cells in the xylem region, but was not detectable in any other tissues of the root and stem tumors.

In this view, supported by only three members of the Court, a power denied by the specific provisions of Article 3, was granted by the generality of Article 1.

they had also had to generalize it -- to the point, finally, where the illusion of depth and relief became abstracted from specific three-dimensional entities and was rendered largely as the illusion of depth and relief as such: as a disembodied attribute and expropriated property detached from everything not itself.

Although the resolution might have been far more specific, it was considerably tougher than the earlier one.

This kind of art goes back to the Minoan period, when its main theme was the representation of motion in a specific moment.

When Austria became a member of the European Union, the Austrian variety of the German language — limited to 23 agricultural terms — was " protected " in Protocol No 10, regarding the use of specific Austrian terms of the German language in the framework of the European Union, which forms part of the Austrian EU accession treaty.

Abbreviations have been used as long as phonetic scripts have existed, in some sense actually being more common in early literacy, where spelling out a whole word was often avoided, initial letters commonly being used to represent words in specific application.

* The Protocol on Environmental Protection to the Antarctic Treaty was signed October 4, 1991 and entered into force January 14, 1998 ; this agreement prevents development and provides for the protection of the Antarctic environment through five specific annexes on marine pollution, fauna and flora, environmental impact assessments, waste management, and protected areas.

The ABC was designed for a specific purpose, the solution of systems of simultaneous linear equations.

Their goal was to regain access to Eden by finding the correct formula for perfect living, following specific rules governing agriculture, diet, and reproduction.

The BATFE has specific design requirements for all firearms sold in the U. S. in order to ensure that they are not readily convertible.

Thus a " Conversation for Action " can be readily tracked and facilitated by a device with little or no ability to model circumstances in the real world other than the ability to register claims by specific agents about a domain.

In other words, there is increasingly a recognition that human capital may be specific to particular jobs or tasks and not general and readily transferable.

Since specific gene therapies cannot readily be used to treat a significant fraction of patients with retinal dystrophy, there is a major interest in developing a more generally applicable survival factor therapy.

Cultivar epithets published before 1 January 1959 were often given a Latin form and can be readily confused with the specific epithets in botanical names ; after that date, newly coined cultivar epithets must be in a modern vernacular language to distinguish them from botanical epithets.

DFA test for the L-type serovar of C trachomatis is the most sensitive and specific test, but is not readily available.

Type 38s in particular were commonly rechambered to " 6. 5 ×. 257 Roberts ", a wildcat cartridge made using the readily available. 257 Roberts cases with the neck expanded to use 6. 5mm bullets specific to the already existing barrel.

The test demonstrated that certain non-weapons specific nuclear technology could be readily turned to weapons development.

As venomous organisms often use their neurotoxins to subdue a predator or prey very rapidly, toxins have evolved to become highly specific to their target channels such that the toxin does not readily bind other targets.

However, most classic car bodies were not designed to be fitted with safety belts and do not possess readily available reinforced mounting points, on the vehicle body, therefore it is very problematic to install such equipment properly: specific studies and calculations should be performed prior to any attempts.

When tested in a simple stimulus matching-to-sample task ( described above ) many animals readily learn specific item combinations, such as " touch red if the sample is red, touch green if the sample is green.

Tayammum is a " dry ablution " using clean soil or dust, to be performed when water is not readily available to perform ablution or when one is defiled ( on janabah ) and could not perform ghusl, and is authorised under specific circumstances.

Attacking this specific enzyme within cancer cells will cause apoptosis to occur more readily due to their high accumulation of DNA damage.

The mineral is readily distinguished from other hydrous manganese oxides ( manganite and wad ) by its greater hardness 5 to 6 ; the specific gravity varies from 3. 7 to 4. 7.

The mineral may be readily recognized by its characteristic twinning, in conjunction with the adamantine lustre and high specific gravity.

:( B ) Reasonably specific enough so that the identity of the signer can be readily recognized.

* An epidural often does not cause as significant neuromuscular block unless specific local anesthetics are used which block motor fibres as readily as sensory nerve fibres, while a spinal more often does.

It is also dependent on a somewhat non-standard character set, with specific characters for assignment ( the right " STO " arrow, not readily available in most character sets ), square and cube roots, and other mathematical symbols, as well as tokenized entry and storage for keywords.

The “ excretory-secretory antigens of larvae … released from their outer epicuticle coat … readily sloughed off when bound by specific antibodies ” incite the host ’ s immune response.

Over the years, Imagineering has conceived a whole range of retail stores, galleries, and hotels that are designed to be experienced and to create and sustain a very specific mood – for example, the mood of Disney's Contemporary Resort could be called “ futuristic optimism ,” and it ’ s readily apparent given the resort ’ s A-frame structure, futuristic building techniques, modern décor, and the monorail gliding quietly through the lobby every few minutes.

The quicksort algorithm is well known and readily available from libraries for software developers writing general-purpose applications in C ++ or Java, but some Javascript implementations do not provide this specific algorithm.

Vertical market software is readily identifiable by the application specific graphical user interface which defines it.

Fog readily assists in creating a specific mood or feeling for a theatrical moment, and is often used in plays, such as Dracula, A Christmas Carol and Macbeth.

These pain patterns in muscles follow specific nerve pathways and have been readily mapped to allow for identification of the causative pain factor.